ACS Sensors,
Год журнала:
2023,
Номер
8(10), С. 3623 - 3642
Опубликована: Окт. 11, 2023
Over
the
past
few
decades,
pathogens
have
posed
a
threat
to
human
security,
and
rapid
identification
of
should
be
one
ideal
methods
prevent
major
public
health
security
outbreaks.
Therefore,
there
is
an
urgent
need
for
highly
sensitive
specific
approaches
identify
quantify
pathogens.
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
CRISPR/Cas
systems
Argonaute
(Ago)
belong
Microbial
Defense
Systems
(MDS).
The
guided,
programmable,
targeted
activation
nucleases
by
both
them
leading
way
new
generation
detection.
We
compare
these
two
in
terms
similarities
differences.
In
addition,
we
discuss
future
challenges
prospects
development
biosensors,
especially
electrochemical
biosensors.
This
review
expected
afford
researchers
entering
this
multidisciplinary
field
useful
guidance
provide
inspiration
more
innovative
biosensors
Analytical Chemistry,
Год журнала:
2023,
Номер
95(14), С. 5927 - 5936
Опубликована: Март 30, 2023
The
aberrant
growth
of
cervical
cells
caused
by
the
infection
human
papillomavirus
(HPV)
may
cause
cancer.
In
order
to
effectively
prevent
occurrence
cancer
and
for
better
follow-up
treatment
after
surgery,
a
rapid
reliable
detection
method
HPV
DNA
is
essential.
Here,
surface-enhanced
Raman
scattering
(SERS)
was
developed
based
on
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/dCas9
technique
enzyme
catalysis
amplification
reaction,
which
achieved
simple
low-content
genes.
CRISPR/dCas9/sgRNA
complex
anchored
above
magnetic
bead,
can
precisely
capture
target
sequences,
exhibiting
high
selectivity
When
biotinylated
DNAs
exist,
they
bridge
streptavidin-modified
horse
radish
peroxidase
(HRP)
producing
an
HRP-decorated
conjugate.
This
conjugate
allows
HRP-catalyzed
reaction
its
substrate
(3,3′,5,5′-tetramethylbenzidine,
TMB).
Gold
nanostars
with
silica
shell
lightning
rod
effect
SERS
were
employed
measure
spectra
oxidative
product
TMB.
Enzyme
co-contribute
signal
output,
ensuring
sensitivity.
proof
concept
detecting
in
system.
current
be
applied
other
simply
changing
sgRNA
sequence.
Many
superiors
portend
that
CRISPR/dCas9-based
promising
further
clinical
application.
ACS Sensors,
Год журнала:
2023,
Номер
8(3), С. 1054 - 1063
Опубликована: Фев. 21, 2023
Detecting
short
genetically
modified
(GM)
nucleic
acid
fragments
in
GM
crops
and
associated
products
is
critically
important
for
the
global
agriculture
industry.
Although
amplification-based
technologies
have
been
widely
used
organism
(GMO)
detection,
they
still
struggle
to
amplify
detect
these
ultra-short
highly
processed
products.
Here,
we
a
multiple-CRISPR-derived
RNA
(crRNA)
strategy
fragments.
By
combining
confinement
effects
on
local
concentrations,
an
amplification-free
CRISPR-based
(CRISPRsna)
system
was
established
cauliflower
mosaic
virus
35S
promoter
samples.
Moreover,
demonstrated
assay
sensitivity,
specificity,
reliability
by
directly
detecting
samples
from
with
wide
genomic
range.
The
CRISPRsna
avoided
possible
aerosol
contamination
amplification
saved
time
due
approach.
Given
that
our
displayed
distinct
advantages
over
other
fragments,
it
may
applications
ACS Sensors,
Год журнала:
2023,
Номер
8(10), С. 3623 - 3642
Опубликована: Окт. 11, 2023
Over
the
past
few
decades,
pathogens
have
posed
a
threat
to
human
security,
and
rapid
identification
of
should
be
one
ideal
methods
prevent
major
public
health
security
outbreaks.
Therefore,
there
is
an
urgent
need
for
highly
sensitive
specific
approaches
identify
quantify
pathogens.
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
CRISPR/Cas
systems
Argonaute
(Ago)
belong
Microbial
Defense
Systems
(MDS).
The
guided,
programmable,
targeted
activation
nucleases
by
both
them
leading
way
new
generation
detection.
We
compare
these
two
in
terms
similarities
differences.
In
addition,
we
discuss
future
challenges
prospects
development
biosensors,
especially
electrochemical
biosensors.
This
review
expected
afford
researchers
entering
this
multidisciplinary
field
useful
guidance
provide
inspiration
more
innovative
biosensors
