Chemosensors,
Год журнала:
2025,
Номер
13(2), С. 60 - 60
Опубликована: Фев. 8, 2025
Pathogens
and
their
metabolites
in
food
present
significant
risks
to
both
human
health
economic
development.
Rising
living
standards
increasing
awareness
of
safety
have
driven
the
demand
for
sensitive
rapid
detection
methods.
Lanthanide-doped
upconversion
nanoparticles
(UCNPs),
with
exceptional
optical
properties,
emerged
as
a
promising
platform
developing
biosensors
detect
pathogenic
bacteria
metabolites.
The
integration
UCNPs
point-of-care
testing
(POCT)
has
garnered
considerable
attention
its
portability
immediacy,
highlighting
future
biosensing,
particularly
applications
requiring
quick
accurate
diagnostics.
This
review
explores
recognition
elements
design
principles
commonly
used
UCNP-based
examines
various
applications,
including
lateral
flow
assays,
microfluidic
systems,
photoelectrochemical
devices,
smartphone-integrated
platforms.
Despite
advancements,
challenges
remain
applicability
commercialization
biosensing
technology.
Future
research
should
focus
on
enhancing
sensitivity
specificity,
scalable
cost-effective
production
methods,
integrating
advanced
digital
technologies
enable
broader
adoption.
Addressing
these
challenges,
establishing
regulatory
frameworks,
considering
sustainability
will
be
crucial
fully
realizing
potential
biosensors.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(35), С. 13368 - 13375
Опубликована: Авг. 23, 2023
A
multifunctional
platform
that
meets
the
demands
of
both
bacterial
detection
and
elimination
is
urgently
needed
because
their
harm
to
human
health.
Herein,
a
"sense-and-treat"
biosensor
was
developed
by
using
immunomagnetic
beads
(IMBs)
AgPt
nanoparticle-decorated
PCN-223-Fe
(AgPt/PCN-223-Fe,
PCN
stands
for
porous
coordination
network)
metal-organic
frameworks
(MOFs).
The
synthesized
AgPt/PCN-223-Fe
not
only
exhibited
excellent
peroxidase-like
activity
but
also
could
efficiently
kill
bacteria
under
near
infrared
(NIR)
irradiation.
This
enabled
colorimetric
E.
coli
O157:H7
in
range
103-108
CFU/mL
with
limit
276
CFU/mL,
accompanied
high
selectivity,
good
reproducibility,
wide
applicability
diverse
real
samples.
Furthermore,
possessed
highly
effective
antibacterial
rate
99.94%
against
808
nm
light
irradiation
20
min.
strategy
can
provide
reference
design
novel
versatile
biosensors
discrimination
applications.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(30), С. 11316 - 11325
Опубликована: Июль 4, 2023
Although
the
traditional
enzyme-linked
immunosorbent
assay
(ELISA)
has
been
widely
applied
in
pathogen
detection
and
clinical
diagnostics,
it
always
suffers
from
complex
procedures,
a
long
incubation
time,
unsatisfying
sensitivity,
single
signal
readout.
Here,
we
developed
simple,
rapid,
ultrasensitive
platform
for
dual-mode
based
on
multifunctional
nanoprobe
integrated
with
capillary
ELISA
(CLISA)
platform.
The
novel
capture
antibodies-modified
capillaries
can
act
as
swab
to
combine
situ
trace
sampling
eliminating
dissociation
between
assays.
With
excellent
photothermal
peroxidase-like
activity,
Fe3O4@MoS2
unique
p–n
heterojunction
was
chosen
an
enzyme
substitute
amplified
tag
label
antibody
further
sandwich
immune
sensing.
As
analyte
concentration
increased,
probe
could
generate
signals,
including
remarkable
color
changes
chromogenic
substrate
oxidation
well
enhancement.
Moreover,
avoid
false
negative
results,
magnetic
capability
of
be
used
pre-enrich
analytes,
amplifying
enhancing
immunoassay's
sensitivity.
Under
optimal
conditions,
specific
rapid
SARS-CoV-2
realized
successfully
this
nanoprobe-enhanced
CLISA
limits
were
5.41
pg·mL–1
150
visual
colorimetric
assay.
More
importantly,
affordable,
portable
also
expanded
rapidly
detect
other
targets
such
Staphylococcus
aureus
Salmonella
typhimurium
practical
samples,
making
universal
attractive
tool
multiple
analysis
testing
post
COVID-19
era.
