Advanced Materials,
Год журнала:
2023,
Номер
36(22)
Опубликована: Ноя. 28, 2023
Abstract
Extracellular
vesicles
(EVs)
are
cell‐derived
nanovesicles
comprising
a
myriad
of
molecular
cargo
such
as
proteins
and
nucleic
acids,
playing
essential
roles
in
intercellular
communication
physiological
pathological
processes.
EVs
have
received
substantial
attention
noninvasive
biomarkers
for
disease
diagnosis
prognosis.
Owing
to
their
ability
recognize
protein
acid
targets,
DNA‐based
nanomaterials
with
excellent
programmability
modifiability
provide
promising
tool
the
sensitive
accurate
detection
carried
by
EVs.
In
this
perspective,
recent
advancements
EV
analysis
using
variety
summarized,
which
can
be
broadly
classified
into
three
categories:
linear
DNA
probes,
nanostructures,
hybrid
nanomaterials.
The
design,
construction,
advantages,
disadvantages
different
types
nanomaterials,
well
performance
detecting
reviewed.
challenges
opportunities
field
also
discussed.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 21, 2025
MicroRNAs
(miRNAs)
are
small
noncoding
RNAs
that
regulate
gene
expression
and
implicated
in
various
diseases,
including
cancer.
Due
to
their
critical
role
diagnostics,
there
is
a
growing
need
for
sensitive,
specific,
rapid
detection
methods
miRNAs.
In
this
study,
we
present
dual-accelerated
signal
amplification
platform
miRNA
biosensing,
which
integrates
spatial
confining
catalytic
hairpin
assembly
(SC-CHA)
with
spherical
CRISPR/Cas12a
(S-CRISPR/Cas12a)
system
(SC-CHA@S-CRISPR/Cas12a)
trans-cleavage
of
DNA
reporters.
The
method
employs
biotinylated
palindrome-rich
sequence
(PAS)
form
nanoballs,
serve
as
scaffold
the
operation
SC-CHA
upon
binding.
products
bind
crRNA
Cas
12a
protein,
activating
S-CRISPR/Cas12a
cleave
reporter
generate
detectable
fluorescence
signal.
uniqueness
lies
combined
use
nanoballs
reporters,
both
significantly
accelerate
reaction
kinetics,
resulting
generation.
Additionally,
nanostructure,
integrated
system,
greatly
enhances
biostability
accelerating
kinetics.
These
features
enable
exhibit
high
sensitivity,
limit
(LOD)
low
13.75
fM,
excellent
specificity,
successfully
distinguishing
miRNA-21
from
other
assay
also
biostable,
demonstrating
reliable
performance
complex
biological
samples
such
human
serum.
This
dual-acceleration
approach
offers
promising
solution
rapid,
specific
potential
applications
early
cancer
diagnosis
clinical
monitoring.
Analytical Chemistry,
Год журнала:
2022,
Номер
94(43), С. 14947 - 14955
Опубликована: Окт. 21, 2022
Proximity-localized
catalytic
hairpin
assembly
(plCHA)
is
intriguing
for
rapid
and
sensitive
assay
of
an
HIV-specific
DNA
segment
(T*).
Using
template-integrated
green
Ag
nanoclusters
(igAgNCs)
as
emitters,
herein,
we
report
the
first
design
a
T*-activated
plCHA
circuit
that
confined
in
three-way-junction
architecture
(3WJA)
fluorescence
sensing
T*.
To
this
end,
T*-recognizable
complement
programmed
stem-loop
(H1),
two
split
template
sequences
igAgNCs
are
separately
overhung
contiguous
to
paired
stems
H1
another
(H2).
The
hybridization
among
H1,
H2,
single-stranded
linkers
(L1
L2)
allows
stable
construction
3WJA.
Upon
presenting
input
T*,
3WJA-localized
operated
through
toehold-mediated
strand
displacements
H2
reactants,
T*
rationally
displaced
repeatably
recycled,
analogous
specific
catalyst,
inducing
more
events.
Resultantly,
hybridized
products
enable
collective
combination
splits
parent
scaffold
hosting
igAgNCs,
outputting
T*-dependent
response.
Because
3WJA
structural
confinement,
spatial
proximity
reactive
hairpins
yielded
high
local
concentrations
manipulate
operation,
achieving
rapider
reaction
kinetics
via
T*-catalyzed
recycling
than
typical
(CHA).
This
simple
strategy
would
open
arena
develop
various
plCHA-based
circuits
capable
modulating
emission
applicable
biosensing
bioanalysis.
