Biosensors,
Год журнала:
2024,
Номер
14(12), С. 633 - 633
Опубликована: Дек. 20, 2024
Antibiotics,
celebrated
as
some
of
the
most
significant
pharmaceutical
breakthroughs
in
medical
history,
are
capable
eliminating
or
inhibiting
bacterial
growth,
offering
a
primary
defense
against
wide
array
infections.
However,
rise
antimicrobial
resistance
(AMR),
driven
by
widespread
use
antibiotics,
has
evolved
into
and
ominous
threat
to
global
public
health.
Thus,
creation
efficient
methods
for
detecting
genes
antibiotics
is
imperative
ensuring
food
safety
safeguarding
human
The
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
CRISPR-associated
proteins
(Cas)
systems,
initially
recognized
an
adaptive
immune
mechanism
bacteria
archaea,
have
unveiled
their
profound
potential
sensor
detection,
transcending
notable
gene-editing
applications.
CRISPR/Cas
technology
employs
Cas
enzymes
guides
RNA
selectively
target
cleave
specific
DNA
sequences.
This
review
offers
extensive
examination
highlighting
unique
attributes
applications
antibiotic
detection.
It
outlines
current
utilization
progress
toolkit
identifying
both
nucleic
acid
(resistance
genes)
non-nucleic
(antibiotic
micromolecules)
targets
within
field
In
addition,
it
examines
challenges,
such
sensitivity
specificity,
future
opportunities,
including
development
point-of-care
diagnostics,
providing
strategic
insights
facilitate
curbing
oversight
antibiotic-resistance
proliferation.
Abstract
DNA
programming,
which
is
based
on
the
principle
of
base
complementary
pairing
and
Boolean
operations,
exhibits
organizational
structures
algorithms
similar
to
those
observed
in
machine
language.
Consequently,
practical
implementation
logic
programming
can
be
achieved
through
utilization
techniques,
enabling
discrimination
output
generation.
In
recent
years,
has
witnessed
a
convergence
with
disciplines,
such
as
life
sciences,
medicine,
other
interdisciplinary
areas,
thereby
giving
rise
an
advanced
research
system
that
yields
valuable
insights.
This
development
paved
way
for
multidisciplinary
cutting‐edge
research.
Furthermore,
successful
transition
from
conceptualization
been
accomplished.
review
summarizes
advances
within
biomedical
fields,
specifically
emphasizing
execution
constructs.
The
benefits
obstacles
associated
adoption
areas
are
also
highlighted.
The FASEB Journal,
Год журнала:
2024,
Номер
38(24)
Опубликована: Дек. 13, 2024
Abstract
Infectious
diseases
are
a
serious
threat
to
the
existence
of
animals
and
humans'
life.
In
21st
century,
emergence
re‐emergence
several
zoonotic
non‐zoonotic
global
pandemic
socio‐economic
importance
has
affected
billions
humans
animals.
The
need
for
expensive
equipment
laboratories,
non‐availability
on‐site
testing
abilities,
with
time‐consuming
low
sensitivity
specificity
issues
currently
available
diagnostic
techniques
identify
these
pathogenic
micro‐organisms
on
large
scale
highlighted
developing
cheap,
portable
environment
friendly
methods.
recent
years,
have
been
addressed
by
clustered
regularly
interspaced
palindromic
repeats
(CRISPR)‐based
platforms
that
transformed
molecular
field
due
their
outstanding
ultra‐sensitive
nucleic
acid
detecting
capabilities.
this
study,
we
highlight
types,
potential
different
Cas
proteins,
amplification
systems.
We
also
illuminate
application
CRISPR
integrated
setups
diagnosis
infectious
diseases,
majorly
in
food‐producing
(pigs,
ruminants,
poultry,
aquaculture),
domestic
pets
(dogs
cats),
importance.
conclude
challenges
future
perspectives
using
systems
rapidly
diagnose
treat
other
develop
control
strategies
prevent
spread
organisms.
Biosensors,
Год журнала:
2024,
Номер
14(12), С. 633 - 633
Опубликована: Дек. 20, 2024
Antibiotics,
celebrated
as
some
of
the
most
significant
pharmaceutical
breakthroughs
in
medical
history,
are
capable
eliminating
or
inhibiting
bacterial
growth,
offering
a
primary
defense
against
wide
array
infections.
However,
rise
antimicrobial
resistance
(AMR),
driven
by
widespread
use
antibiotics,
has
evolved
into
and
ominous
threat
to
global
public
health.
Thus,
creation
efficient
methods
for
detecting
genes
antibiotics
is
imperative
ensuring
food
safety
safeguarding
human
The
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
CRISPR-associated
proteins
(Cas)
systems,
initially
recognized
an
adaptive
immune
mechanism
bacteria
archaea,
have
unveiled
their
profound
potential
sensor
detection,
transcending
notable
gene-editing
applications.
CRISPR/Cas
technology
employs
Cas
enzymes
guides
RNA
selectively
target
cleave
specific
DNA
sequences.
This
review
offers
extensive
examination
highlighting
unique
attributes
applications
antibiotic
detection.
It
outlines
current
utilization
progress
toolkit
identifying
both
nucleic
acid
(resistance
genes)
non-nucleic
(antibiotic
micromolecules)
targets
within
field
In
addition,
it
examines
challenges,
such
sensitivity
specificity,
future
opportunities,
including
development
point-of-care
diagnostics,
providing
strategic
insights
facilitate
curbing
oversight
antibiotic-resistance
proliferation.
