Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Июнь 24, 2024
Nucleic
acid
amplification
testing
has
great
potential
for
point-of-need
diagnostic
with
high
detection
sensitivity
and
specificity.
Current
sample
preparation
is
limited
by
a
tedious
workflow
requiring
multiple
steps,
reagents
instrumentation,
hampering
nucleic
at
point
of
need.
In
this
study,
we
present
the
use
mixed
cellulose
ester
(MCE)
paper
DNA
binding
ionic
interaction
under
molecular
crowding
conditions
fluid
transport
wicking.
The
poly(ethylene)
glycol-based
(PEG)
reagent
simultaneously
provides
pH
alkaline
lysis
effects
salt
conditions.
introduce
Paper-based
Abridged
Solid-Phase
Extraction
Alkaline
Poly(ethylene)
Glycol
Lysis
(PASAP).
anionic
used
as
solid
phase
allows
wicking,
eliminating
need
pipetting
skills
magnet
to
retain
beads.
Following
release
from
cells
due
lytic
activity
PASAP
solution,
binds
surface
MCE
paper,
concentrating
bottom
while
matrix
transported
towards
top
was
washed
dipping
it
in
40%
isopropanol
10
s.
After
air-drying
30
s,
section
(3
mm
×
4
mm)
snapped
off
using
cap
PCR
tube
immersed
colourimetric
loop-mediated
isothermal
(cLAMP)
solution
direct
detection.
total
processing
completed
15
min
ready
amplification.
cLAMP
enabled
102
CFU/mL
Escherichia
coli
(E.
coli)
culture
media
E.
milk
<
103
(10
CFU)
after
incubation
68
°C
60
min,
demonstrating
applicability
method
complex
biological
samples.
Molecules,
Год журнала:
2024,
Номер
29(11), С. 2439 - 2439
Опубликована: Май 22, 2024
The
measurement
of
glucose
concentration
is
a
fundamental
daily
care
for
diabetes
patients,
and
therefore,
its
detection
with
accuracy
prime
importance
in
the
field
health
care.
In
this
study,
fabrication
an
electrochemical
sensor
sensing
was
successfully
designed.
electrode
material
fabricated
using
polyaniline
systematically
characterized
scanning
electron
microscopy,
high-resolution
transmission
X-ray
diffraction,
Fourier
transform
infrared
spectroscopy,
UV-visible
spectroscopy.
nanofiber-modified
showed
excellent
ability
linear
range
10
μM
to
1
mM
limit
10.6
μM.
stability
same
tested
7
days.
shows
high
sensitivity
presence
interferences.
polyaniline-modified
does
not
affect
interferences
has
low
limit.
It
also
cost-effective
require
complex
sample
preparation
steps.
This
makes
it
potential
tool
pharmacy
medical
diagnostics.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Июнь 24, 2024
Nucleic
acid
amplification
testing
has
great
potential
for
point-of-need
diagnostic
with
high
detection
sensitivity
and
specificity.
Current
sample
preparation
is
limited
by
a
tedious
workflow
requiring
multiple
steps,
reagents
instrumentation,
hampering
nucleic
at
point
of
need.
In
this
study,
we
present
the
use
mixed
cellulose
ester
(MCE)
paper
DNA
binding
ionic
interaction
under
molecular
crowding
conditions
fluid
transport
wicking.
The
poly(ethylene)
glycol-based
(PEG)
reagent
simultaneously
provides
pH
alkaline
lysis
effects
salt
conditions.
introduce
Paper-based
Abridged
Solid-Phase
Extraction
Alkaline
Poly(ethylene)
Glycol
Lysis
(PASAP).
anionic
used
as
solid
phase
allows
wicking,
eliminating
need
pipetting
skills
magnet
to
retain
beads.
Following
release
from
cells
due
lytic
activity
PASAP
solution,
binds
surface
MCE
paper,
concentrating
bottom
while
matrix
transported
towards
top
was
washed
dipping
it
in
40%
isopropanol
10
s.
After
air-drying
30
s,
section
(3
mm
×
4
mm)
snapped
off
using
cap
PCR
tube
immersed
colourimetric
loop-mediated
isothermal
(cLAMP)
solution
direct
detection.
total
processing
completed
15
min
ready
amplification.
cLAMP
enabled
102
CFU/mL
Escherichia
coli
(E.
coli)
culture
media
E.
milk
<
103
(10
CFU)
after
incubation
68
°C
60
min,
demonstrating
applicability
method
complex
biological
samples.
