Analytical Chemistry,
Год журнала:
2023,
Номер
95(37), С. 13855 - 13863
Опубликована: Сен. 6, 2023
Bacterial
infections
seriously
harm
human
health
and
cause
many
severe
diseases,
which
triggered
urgent
demands
to
exploit
specific
sensitive
biosensor
strategies
for
Staphylococcus
aureus
detection.
Here,
a
colorimetric
photoelectrochemical
dual-mode
S.
assay
based
on
FePor-TPA
was
constructed.
2D
thin
film
its
bulk
powder
(FePor-TPA)
were
synthesized
by
in
situ
growth
ITO
solvothermal
condition,
respectively,
both
of
exhibited
excellent
peroxidase-like
catalase-like
activity,
originating
from
their
metalloporphyrin
linkers.
Benefiting
the
electrodes,
also
possessed
more
ordered
stacking
mode
turn
good
electrical
conductivity,
stable
initial
photocurrent,
high
sensitivity
O2.
As
FePor-TPA,
porous
structure
surface
area
make
it
possible
scaffold
load
an
amount
AuNPs,
rabbit
anti-Staphylococcus
Rosenbach
tropina
antibody
(Ab2),
GOx
constructing
signal
probe
(GOx/Ab2@Au@FePor-TPA)
realizing
catalytic
amplification.
With
these
satisfactory
features
mind,
utilized
construct
dual
signal-on
bioplatform
sensitively
selectively
detecting
aureus,
which,
as
far
we
know,
has
not
been
reported.
Advanced Functional Materials,
Год журнала:
2023,
Номер
33(46)
Опубликована: Июль 12, 2023
Abstract
Non‐fullerene
acceptors
(NFAs)
are
a
crucial
component
of
organic
photovoltaics,
and
they
have
gained
significant
attention
due
to
their
outstanding
photoelectric
conversion
efficiency.
However,
the
recognition
reactions
specific
building
blocks
in
NFAs
largely
overlooked
construction
photoelectrochemical
(PEC)
biosensing
platforms.
In
this
study,
potential
Y6,
prototype
NFA,
is
explored
construct
sensitive
PEC
biosensor
for
monitoring
urease
activity
selective
chemical
reactivity
its
blocks.
The
resultant
relies
on
urease‐mediated
enzymatic
reaction,
which
produces
OH
−
anions
that
act
as
nucleophilic
reagent
linkage
C═C
Y6
moiety.
This
results
formation
Y6‐OH,
exhibits
depressive
response
destroyed
conjugated
structure
intramolecular
charge
transfer.
As
expected,
linear
relationship
observed
between
recession
performance
concentration
urease,
with
good
sensitivity
selectivity.
Furthermore,
detection
also
successfully
realized
human
saliva
samples,
suggesting
promising
NFA‐based
biosensors
clinical
applications
even
absence
common
biological
units.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(37), С. 13855 - 13863
Опубликована: Сен. 6, 2023
Bacterial
infections
seriously
harm
human
health
and
cause
many
severe
diseases,
which
triggered
urgent
demands
to
exploit
specific
sensitive
biosensor
strategies
for
Staphylococcus
aureus
detection.
Here,
a
colorimetric
photoelectrochemical
dual-mode
S.
assay
based
on
FePor-TPA
was
constructed.
2D
thin
film
its
bulk
powder
(FePor-TPA)
were
synthesized
by
in
situ
growth
ITO
solvothermal
condition,
respectively,
both
of
exhibited
excellent
peroxidase-like
catalase-like
activity,
originating
from
their
metalloporphyrin
linkers.
Benefiting
the
electrodes,
also
possessed
more
ordered
stacking
mode
turn
good
electrical
conductivity,
stable
initial
photocurrent,
high
sensitivity
O2.
As
FePor-TPA,
porous
structure
surface
area
make
it
possible
scaffold
load
an
amount
AuNPs,
rabbit
anti-Staphylococcus
Rosenbach
tropina
antibody
(Ab2),
GOx
constructing
signal
probe
(GOx/Ab2@Au@FePor-TPA)
realizing
catalytic
amplification.
With
these
satisfactory
features
mind,
utilized
construct
dual
signal-on
bioplatform
sensitively
selectively
detecting
aureus,
which,
as
far
we
know,
has
not
been
reported.
