Foods,
Год журнала:
2024,
Номер
13(21), С. 3416 - 3416
Опубликована: Окт. 26, 2024
Foodborne
illnesses
pose
a
serious
threat
to
public
health,
with
increasing
global
incidence
rates
driven
by
factors
such
as
rising
meat
consumption.
Rapid
detection
of
foodborne
pathogens
in
is
critical
for
preventing
outbreaks.
This
study
investigates
the
potential
gas
chromatography-mass
spectrometry
(GC-MS)
and
Fourier-transform
infrared
spectroscopy
(FTIR)
identifying
biomarkers
spectral
fingerprints
indicative
raw
chicken
meat.
Raw
broiler
samples
were
surface-sterilized
inoculated
pathogens.
The
challenge
specific
pathogen
physical
quality
parameters
like
pH,
color,
texture,
drip
loss,
water
activity
assessed.
GC-MS
analysis
identified
113
metabolites,
including
ureidopropionic
acid,
5-sulfosalicylic
11,14-eicosadienoic
methyl
ester
E.
coli
O157:H7;
11-bromoundecanoic
neocurdione,
glafenin,
eicosanoic
acid
Salmonella;
azepan-1-yl-acetic
ester,
tramadol,
cytarabine,
dipipanone
Staphylococcus
cyclopentaneundecanoic
phosphonofluoridic
î-n-formyl-l-lysine
Pseudomonas.
Pathway
revealed
involvement
fatty
metabolism
amino
degradation
pathways.
FTIR
data
showed
significant
variances
between
control
spiked
samples,
particularly
region.
metabolites
patterns
could
serve
developing
rapid
methods,
contributing
enhanced
food
safety
protocols.
Fluid
manipulation
is
an
important
foundation
of
microfluidic
technology.
Various
methods
and
devices
have
been
developed
for
fluid
control,
such
as
electrowetting-on-dielectric-based
digital
platforms,
pumps,
pneumatic
valves.
These
enable
precise
small
volumes
fluids.
However,
their
complexity
high
cost
limit
the
commercialization
widespread
adoption
Shape
memory
polymers
smart
materials
can
adjust
shape
in
response
to
external
stimuli.
By
integrating
into
chips,
new
possibilities
expanding
application
areas
technology
emerge.
serve
actuators
or
regulators
drive
control
flow
systems,
offering
innovative
approaches
manipulation.
Due
unique
properties,
provide
a
solution
construction
intelligent
automated
systems.
chips
are
expected
be
one
future
directions
development
This
article
offers
summary
recent
research
achievements
field
droplet
provides
insights
direction
devices.
In
this
work,
efficient
microfluidic
flow
rate
tuning
based
on
the
asymmetric
of
nematic
liquid
crystal
5CB
under
a
horizontal
temperature
gradient
is
studied.
Rectangular
microchannels
with
width
100
μm
are
fabricated
through
soft
lithography
and
treated
homeotropic
surface
anchoring
conditions.
Polarized
optical
microscopy
applied
to
explore
unique
anisotropic
characteristics
crystal.
The
velocity
profiles
in
microchannel
obtained
by
particle
tracking
velocimetry.
effects
temperature,
rate,
aspect
ratio
profile
split
quantitatively
studied
for
first
time,
while
mechanism
asymmetry
discussed.
results
show
that
occurs
after
applied,
heated
region
markedly
higher
than
its
counterpart.
increases
increase
decrease
rate.
influences
approaches
average
strength,
former
more
distinct.
impacts
gradient,
crystals
caused
coupling
between
physical
properties,
field,
director
field.
Microchannels
can
act
as
novel
kind
temperature-controlled
microvalve
achieve
tuning.
Abstract
While
fluorescence‐based
high‐throughput
screening
(HTS)
has
been
valuable
for
identifying
enzymes
and
microorganisms
polyethylene
terephthalate
(PET)
depolymerization,
the
traditional
approach
relies
on
cleaving
ester
bonds
in
small‐molecule
probes,
limiting
its
ability
to
directly
detect
activity
towards
polymeric
PET
substrates.
This
study
proposes
a
novel
strategy
detecting
release
of
sulfhydryl
groups
during
depolymerization
pseudo‐PET
polythioesters
by
polyester
hydrolases.
The
successfully
identifies
polyester‐depolymerizing
leaf‐branch
compost
cutinase
(LCC
ICCG
),
whereas
porcine
liver
esterase
(PLE)
only
hydrolyses
small
molecular
Furthermore,
can
be
integrated
with
droplet
microfluidic
system
LCC
.
work
further
demonstrates
applicability
microplate
reader
platform
bacteria.
offers
an
efficient
identify
microbial
resources
depolymerizing
polyester‐like
plastics.
Biosensors,
Год журнала:
2024,
Номер
14(9), С. 408 - 408
Опубликована: Авг. 23, 2024
Due
to
the
extremely
low
Reynolds
number,
mixing
of
substances
in
laminar
flow
within
microfluidic
channels
primarily
relies
on
slow
intermolecular
diffusion,
whereas
various
rapid
reaction
and
detection
requirements
lab-on-a-chip
applications
often
necessitate
efficient
fluids
short
distances.
This
paper
presents
a
magnetic
pillar-shaped
particle
fabrication
device
capable
producing
particles
with
planar
shapes,
which
are
then
utilized
achieve
multiple
microchannels.
During
process,
degassed
PDMS
chip
provides
self-priming
capabilities,
drawing
UV-curable
adhesive-containing
powder
distributing
it
into
distinct
microwell
structures.
Subsequently,
an
external
field
is
applied,
exposed
UV
light,
enabling
mass
production
specific
properties
through
photo-curing.
Without
need
for
pumping,
this
chip-based
can
fabricate
hundreds
less
than
10
min.
In
contrast
most
methods,
approach
enables
precise
dispensing,
allowing
control
over
shape
size.
The
fabricated
dual-layer
particles,
featuring
fan-shaped
blades
disk-like
structures,
placed
micromixing
channels.
By
manipulating
field,
driven
motion,
altering
patterns
fluid
mixing.
Under
conditions
where
number
ranges
from
0.1
0.9,
index
aqueous
solutions
exceeds
0.9.
addition,
experimental
analyses
efficiency
different
viscosities,
including
25
wt%
50
glycerol,
reveal
indices
exceeding
0.85,
demonstrating
broad
applicability
micromixers
based
rotation
particles.
Foods,
Год журнала:
2024,
Номер
13(21), С. 3416 - 3416
Опубликована: Окт. 26, 2024
Foodborne
illnesses
pose
a
serious
threat
to
public
health,
with
increasing
global
incidence
rates
driven
by
factors
such
as
rising
meat
consumption.
Rapid
detection
of
foodborne
pathogens
in
is
critical
for
preventing
outbreaks.
This
study
investigates
the
potential
gas
chromatography-mass
spectrometry
(GC-MS)
and
Fourier-transform
infrared
spectroscopy
(FTIR)
identifying
biomarkers
spectral
fingerprints
indicative
raw
chicken
meat.
Raw
broiler
samples
were
surface-sterilized
inoculated
pathogens.
The
challenge
specific
pathogen
physical
quality
parameters
like
pH,
color,
texture,
drip
loss,
water
activity
assessed.
GC-MS
analysis
identified
113
metabolites,
including
ureidopropionic
acid,
5-sulfosalicylic
11,14-eicosadienoic
methyl
ester
E.
coli
O157:H7;
11-bromoundecanoic
neocurdione,
glafenin,
eicosanoic
acid
Salmonella;
azepan-1-yl-acetic
ester,
tramadol,
cytarabine,
dipipanone
Staphylococcus
cyclopentaneundecanoic
phosphonofluoridic
î-n-formyl-l-lysine
Pseudomonas.
Pathway
revealed
involvement
fatty
metabolism
amino
degradation
pathways.
FTIR
data
showed
significant
variances
between
control
spiked
samples,
particularly
region.
metabolites
patterns
could
serve
developing
rapid
methods,
contributing
enhanced
food
safety
protocols.
