BBA Advances, Год журнала: 2025, Номер 7, С. 100151 - 100151
Опубликована: Янв. 1, 2025
Язык: Английский
Baghdad Journal of Biochemistry and Applied Biological Sciences, Год журнала: 2025, Номер 5(4)
Опубликована: Янв. 20, 2025
Polymerase Chain Reaction (PCR) techniques have revolutionized forensic DNA analysis, enabling the precise amplification of trace samples. This abstract provides a concise overview pivotal role PCR in science. It delves into principles PCR, emphasizing its ability to amplify specific sequences with remarkable sensitivity and specificity. Multiplex variant technique, allows for simultaneous multiple genetic loci, enhancing efficiency investigations. further highlights broad applications criminal investigations, paternity testing, disaster victim identification. Recent advancements, such as MiniSTRs Next-Generation Sequencing (NGS), are elucidated their crucial contributions addressing challenging scenarios. Despite instrumental role, PCR-based analysis is not without challenges, considerations including contamination low-template The concludes aforward-looking perspective on emerging field epigenetics metagenomic offering glimpse promising future analysis. stands an indispensable tool modern science, unraveling identities from most minute traces.
Язык: Английский
Процитировано
0
Molecular Biotechnology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 22, 2025
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140079 - 140079
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Фев. 3, 2025
Transplant rejection remains a significant barrier to the long-term success of organ transplantation. Biopsy, although considered gold standard, is invasive, costly, and unsuitable for routine monitoring. Traditional biomarkers, such as creatinine troponin, offer limited predictive value owing their low specificity, conventional imaging techniques often fail detect early damage, increasing risk undiagnosed episodes. Considering these limitations, emerging noninvasive biomarkers molecular hold promise accurate detection transplant rejection, enabling personalized management strategies. This review highlights that predict, diagnose, assess prognosis by reflecting graft injury, inflammation, immune responses. For example, donor-derived cell-free DNA (dd-cfDNA) highly sensitive in detecting whereas gene expression profiling effectively excludes moderate-to-severe acute (AR). Additionally, microRNA (miRNA) enhances diagnostic specificity precise AR detection. Advanced further augment monitoring rejection. Fluorescence provides high spatiotemporal resolution grading, ultrasound offers real-time portable monitoring, magnetic resonance delivers tissue contrast anatomical assessments. Nuclear modalities single photon emission computed tomography positron tomography, enable dynamic visualization responses within transplanted organs. Notably, dd-cfDNA nuclear medicine have already been integrated into clinical practice, thereby demonstrating translational potential techniques. Unlike previous reviews, this work uniquely synthesizes advancements both imaging, emphasizing complementary strengths. Biomarkers deliver molecular-level insights, spatial temporal resolution. Together, they create synergistic framework comprehensive By bridging domains, underscores individual contributions collective enhance accuracy, improve patient outcomes, guide future research applications medicine.
Язык: Английский
Процитировано
0
BBA Advances, Год журнала: 2025, Номер 7, С. 100151 - 100151
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0