Biosensors,
Год журнала:
2023,
Номер
13(8), С. 826 - 826
Опубликована: Авг. 17, 2023
Overweight
and
obesity
promote
diabetes
heart
disease
onset.
Triglycerides
are
key
biomarkers
for
cardiovascular
disease,
strokes,
other
health
issues.
Scientists
have
devised
methods
instruments
the
detection
of
these
molecules
in
liquid
samples.
In
this
study,
an
enzymatic
biosensor
was
developed
using
Arduino-based
microfluidic
platform,
wherein
a
lipolytic
enzyme
immobilized
on
ethylene-vinyl
acetate
polymer
through
physical
adsorption.
This
low-cost
optical
employed
spectrophotometric
transducer
assessed
samples
to
indirectly
detect
triglycerides
fatty
acids
p-nitrophenol
as
indicator.
The
average
triglyceride
level
detected
conducted
experiments
47.727
mg/dL.
exhibited
percentage
recovery
81.12%
variation
coefficient
0.791%.
Furthermore,
demonstrated
ability
levels
without
need
sample
dilution,
ranging
from
7.6741
mg/dL
58.835
study
successfully
efficient
affordable
prototype
acid
detection.
immobilization
substrate
provided
stable
reproducible
system,
rendering
exciting
option
molecules.
Molecules,
Год журнала:
2024,
Номер
29(9), С. 2021 - 2021
Опубликована: Апрель 27, 2024
Bioremediation
uses
the
degradation
abilities
of
microorganisms
and
other
organisms
to
remove
harmful
pollutants
that
pollute
natural
environment,
helping
return
it
a
state
is
free
substances.
Organism-derived
enzymes
can
degrade
eliminate
variety
transform
them
into
non-toxic
forms;
as
such,
they
are
expected
be
used
in
bioremediation.
However,
since
proteins,
low
operational
stability
catalytic
efficiency
enzyme-based
systems
need
improvement.
Enzyme
immobilization
methods
often
overcome
these
challenges.
Several
enzyme
have
been
applied
improve
reduce
remediation
costs.
Herein,
we
review
recent
advancements
immobilized
for
bioremediation
summarize
preparing
use
catalysts
pollutant
systems.
Additionally,
advantages,
limitations,
future
perspectives
discussed.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(30)
Опубликована: Апрель 15, 2024
Abstract
Microfluidic
systems
have
become
integral
for
lab‐on‐a‐chip
and
organ‐on‐a‐chip
applications
across
numerous
disciplines.
These
systems,
typically
fabricated
using
polydimethylsiloxane
(PDMS)
chips
on
glass
substrates,
lack
the
bioactivity
required
such
applications.
To
overcome
this,
biomolecules
are
immobilized
either
oxygen
(O
2
)
plasma
treatment
or
chemical
reagents
like
amino
silanes.
However,
O
treatments
unstable
cannot
covalently
immobilize
biomolecules,
while
wet‐chemistry
approaches
toxic,
time‐consuming,
expensive.
A
novel
microfluidic
platform
that
combines
two
surface
treatments:
Plasma‐activated
coating
(PAC)
atmospheric
pressure
jet
(APPJ),
to
enable
reagent‐free
covalent
immobilization
of
is
described
here.
treatments,
unlike
plasma,
fibronectin
PDMS
glass,
significantly
improved
endothelial
cell
attachment
proliferation.
By
combining
PAC
APPJ,
a
hybrid
with
equivalent
bond
strength
standard
devices,
but
enhanced
growth
in
artery‐on‐a‐chip
model,
developed.
This
also
amenable
high‐shear
as
coronary
shear,
cells
aligning
flow,
seen
human
arteries.
providing
within
system,
this
technology
has
potential
radically
improve
development
well
point‐of‐care
diagnostics,
sensors.
Journal of Biotechnology,
Год журнала:
2024,
Номер
382, С. 88 - 96
Опубликована: Янв. 26, 2024
L-Lactate
oxidase
has
important
applications
in
biosensing
and
finds
increased
use
biocatalysis.
The
enzyme
been
characterized
well,
yet
its
immobilization
not
explored
depth.
Here,
we
studied
of
Aerococcus
viridans
L-lactate
on
porous
carriers
variable
matrix
material
(polymethacrylate,
polyurethane,
agarose)
surface
functional
group
(amine,
Ni2+-loaded
nitrilotriacetic
acid
(NiNTA),
epoxide).
Carrier
activity
(Ac)
immobilized
effectiveness
(η)
were
evaluated
dependence
protein
loading.
Results
show
that
efficient
(Ac:
up
to
1,450
U/g
carrier;
η:
65%)
requires
a
hydrophilic
carrier
(agarose)
equipped
with
amine
groups.
value
η
declines
sharply
as
Ac
increases,
probably
due
transition
into
diffusional
regime.
Untagged
binds
NiNTA
similarly
N-terminally
His-tagged
enzyme.
Lixiviation
studies
reveal
quasi-irreversible
adsorption
while
partial
release
(≤
25%)
is
shown
from
carrier.
desorbed
exhibits
the
same
specific
original
oxidase.
Collectively,
our
study
identifies
basic
requirements
solid
highlights
role
ionic
interactions
enzyme-surface
adsorption.
Biosensors,
Год журнала:
2025,
Номер
15(2), С. 81 - 81
Опубликована: Фев. 1, 2025
Glycine
is
an
essential
amino
acid
involved
in
synthesizing
a
variety
of
important
biomolecules,
and
its
concentration
can
influence
numerous
biochemical
processes,
including
the
severity
symptoms
wide
range
conditions
humans,
such
as
cancer,
schizophrenia,
major
depression,
diabetes.
While
few
costly
or
labour-intensive
methods
are
currently
available,
we
have
developed
new
enzymatic
biosensor
that
accurately
measure
glycine
levels
with
remarkable
simplicity.
By
employing
immobilized
laccase
enzymes
combination
surface
plasmon
resonance
(SPR)
device,
our
system
achieved
limit
detection
(LOD)
9.95
mM
quantification
(LOQ)
33.19
mM.
In
addition,
it
demonstrated
recovery
rate
97.64
±
7.71%.
Moreover,
maintained
consistent
signal
intensity
over
21
days
supported
total
60
analyses
using
same
enzyme
setup,
demonstrating
excellent
reusability.
Notably,
this
study
marks
first
time
has
been
determined
SPR-based
platform.
