Biosensors,
Год журнала:
2023,
Номер
13(8), С. 826 - 826
Опубликована: Авг. 17, 2023
Overweight
and
obesity
promote
diabetes
heart
disease
onset.
Triglycerides
are
key
biomarkers
for
cardiovascular
disease,
strokes,
other
health
issues.
Scientists
have
devised
methods
instruments
the
detection
of
these
molecules
in
liquid
samples.
In
this
study,
an
enzymatic
biosensor
was
developed
using
Arduino-based
microfluidic
platform,
wherein
a
lipolytic
enzyme
immobilized
on
ethylene-vinyl
acetate
polymer
through
physical
adsorption.
This
low-cost
optical
employed
spectrophotometric
transducer
assessed
samples
to
indirectly
detect
triglycerides
fatty
acids
p-nitrophenol
as
indicator.
The
average
triglyceride
level
detected
conducted
experiments
47.727
mg/dL.
exhibited
percentage
recovery
81.12%
variation
coefficient
0.791%.
Furthermore,
demonstrated
ability
levels
without
need
sample
dilution,
ranging
from
7.6741
mg/dL
58.835
study
successfully
efficient
affordable
prototype
acid
detection.
immobilization
substrate
provided
stable
reproducible
system,
rendering
exciting
option
molecules.
Chemosensors,
Год журнала:
2024,
Номер
12(6), С. 98 - 98
Опубликована: Июнь 4, 2024
Simple
and
reliable
electrochemical
sensors
are
highly
demanded
in
medicine
pharmacy
for
the
fast
determination
of
metabolites
biomarkers
diseases.
In
this
work,
a
flow-through
biosensor
system
was
developed
on
base
screen-printed
carbon
electrode
modified
with
pillar[3]arene[2]quinone
ferrocene
implemented
black.
The
modification
performed
single
step
resulted
formation
stable
layer
good
operation
characteristics.
Uricase
immobilized
inner
walls
replaceable
reactor
by
carbodiimide
binding.
A
cell
manufactured
3D
printing
from
poly(lactic
acid).
first
optimized
hydrogen
peroxide
assay
then
used
1
nM–0.1
mM
uric
acid
(limit
detection
0.3
nM,
20
measurements
per
hour).
Implementation
synergetic
increase
cathodic
current
H2O2
reduction
measured
flow
switching
chronoamperometric
mode.
tested
artificial
urine
Ringer–Locke
solution
showed
recovery
rate
96–112%.
addition,
possibility
commercial
disinfectants
shown.
Easy
assembly,
signal
low
consumption
reagents
make
attractive
routine
clinical
analysis
metabolites.
Biosensors,
Год журнала:
2023,
Номер
13(8), С. 826 - 826
Опубликована: Авг. 17, 2023
Overweight
and
obesity
promote
diabetes
heart
disease
onset.
Triglycerides
are
key
biomarkers
for
cardiovascular
disease,
strokes,
other
health
issues.
Scientists
have
devised
methods
instruments
the
detection
of
these
molecules
in
liquid
samples.
In
this
study,
an
enzymatic
biosensor
was
developed
using
Arduino-based
microfluidic
platform,
wherein
a
lipolytic
enzyme
immobilized
on
ethylene-vinyl
acetate
polymer
through
physical
adsorption.
This
low-cost
optical
employed
spectrophotometric
transducer
assessed
samples
to
indirectly
detect
triglycerides
fatty
acids
p-nitrophenol
as
indicator.
The
average
triglyceride
level
detected
conducted
experiments
47.727
mg/dL.
exhibited
percentage
recovery
81.12%
variation
coefficient
0.791%.
Furthermore,
demonstrated
ability
levels
without
need
sample
dilution,
ranging
from
7.6741
mg/dL
58.835
study
successfully
efficient
affordable
prototype
acid
detection.
immobilization
substrate
provided
stable
reproducible
system,
rendering
exciting
option
molecules.
