Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Electrophoresis, Год журнала: 2023, Номер 45(1-2), С. 165 - 198

Опубликована: Сен. 5, 2023

Abstract This review article brings a comprehensive survey of developments and applications high‐performance capillary microchip electromigration methods (zone electrophoresis in free solution or sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, electrochromatography) for analysis, micropreparation, physicochemical characterization peptides the period from 2021 up to ca. middle 2023. Progress study properties various aspects their such as sample preparation, adsorption suppression, electroosmotic flow regulation, detection, are presented. New particular demonstrated, several types reported. They cover qualitative quantitative analysis synthetic isolated determination complex biomatrices, peptide profiling biofluids tissues, monitoring chemical enzymatic reactions changes peptides. include also amino acid sequence peptides, mapping proteins, separation stereoisomers chiral analyses. In addition, micropreparative separations interactions with other (bio)molecules by above CE described.

Язык: Английский

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Ionic liquids in capillary electrophoresis analysis of proteins and carbohydrates

Journal of Chromatography A, Год журнала: 2024, Номер 1716, С. 464642 - 464642

Опубликована: Янв. 11, 2024

Язык: Английский

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Greening Separation and Purification of Proteins and Peptides

Journal of Separation Science, Год журнала: 2024, Номер 47(19)

Опубликована: Окт. 1, 2024

The increasing awareness of environmental issues and the transition to green analytical chemistry (GAC) have gained popularity among academia industry in recent years. One principles GAC is reduction replacement toxic solvents with more sustainable environmentally friendly ones. This review gives an overview advances applying as alternative traditional organic for peptide protein purification analysis by liquid chromatography (LC) capillary electrophoresis (CE) methods. feasibility using greener LC CE methods demonstrated through several application examples; however, there still plenty room new developments fully realize their potential address existing challenges. Thanks tunable properties designer solvents, such ionic liquids deep eutectic almost infinite possible mixtures components production, it that some could potentially surpass harmful future. Therefore, future research should focus mainly on developing solvent combinations enhancing instruments be able effectively work solvents.

Язык: Английский

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Determination of amino acids and peptides without their pre‐column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview

Journal of Separation Science, Год журнала: 2024, Номер 47(16)

Опубликована: Авг. 1, 2024

This review provides an overview of recent works focusing on the determination amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection ultraviolet (UV) detection, which is most widespread in electromigration techniques, without pre-capillary derivatization. Available options for UV these analytes, such as indirect complexation transition metal ions, in-capillary derivatization are described. Developments field direct UV-absorbing AAs well progress chiral separation A separate section dedicated to on-line sample preconcentration methods combined electrophoresis-UV.

Язык: Английский

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Surfactant-based coatings for protein separation by capillary electrophoresis - A review

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 343945 - 343945

Опубликована: Март 1, 2025

Язык: Английский

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Separation of Virus Like Particles and Nano-Emulsions for Vaccine Development by Capillary Zone Electrophoresis

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 344011 - 344011

Опубликована: Апрель 1, 2025

Язык: Английский

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Chiral Separation Enhancement in Capillary Electrophoresis by Electrophoretic Mobility Differences without Electroosmosis

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 13, 2025

To improve separation efficiency and address the adverse effects of electro-osmosis, a coated capillary was used to shield electroosmosis. A nanoflow pump control flow electrophoretic medium, thereby extending time action. Furthermore, by using that is only 10 cm long as column, electric field strength could reach high 250 V/mm. These strategies strengthened interaction between enantiomers chiral selector, thus enhancing resolution enantiomers. As result, complete amlodipine be achieved halting medium. Further duration stoppage can increase several-fold. The method established in this study exhibits excellent accuracy precision. Despite experiencing period stoppage, relative standard deviations both migration remain less than 2%. This not remarkably enhanced but also demonstrated broad applicability separating other enantiomers, particularly for those substances are difficult separate conventional electrophoresis.

Язык: Английский

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Development of a capillary zone electrophoresis method to monitor magnesium ion consumption during in vitro transcription for mRNA production

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер 416(12), С. 2941 - 2949

Опубликована: Апрель 10, 2024

Язык: Английский

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Progress of highly reproducible capillary electrophoresis

Chinese Journal of Chromatography, Год журнала: 2024, Номер 42(6), С. 544 - 554

Опубликована: Июнь 1, 2024

Following rapid developments in capillary electrophoresis (CE), this technology has become an established analytical technique owing to its microscale characteristics, high speed, efficiency, and versatility. However, the challenges of poor peak stability and/or reproducibility have consistently hindered wider applications. CE long been used as a measurement tool for plotting signal intensities versus migration time; however, time is not independent variable CE, but affected by many direct indirect parameters, including (length, diameter, inner surface properties), electric field (or voltage, current, power), temperature, running buffer (electrolytes, additives, solvents, their concentration, buffering pH, etc.). These intricacies render acquisition reproducible electropherograms difficult. Various studies ranging from those on early stages development exploration three important strategies conducted address issue. In first strategy, conditions, especially parameters that can maintain stable electro-osmotic flow, are strictly controlled stabilized significantly improve repeatability. second either position corrected using internal standards or converted into other variables, such electrophoretic mobility, offset eliminate some unstable factors, thereby improving repeatability even peaks; strategy useful when signals ratio, correlated time, effective temperature-correlated mobility. third new methodology called highly (HRCE) theoretical explore better principles real-time with aim complete removal challenge root. This includes novel methods plot based weighted migrated charge, charge density, partial differential molar density. Similar ordinary approaches, also draw ratios these properties. As theoretically predicted, resist changes critical conditions (mainly strength, length concentration). Our experimental results demonstrate given certain prerequisites, set produce electropherograms. review focuses basis advancements HRCE, elucidates link between migration/peak expression theories impact reproducibility. Studies transformation time-scale literature summarized analyzed general. does directly discuss research progress through instrument upgrades, parameter optimization, practical method refinements.

Язык: Английский

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Capillary Electrophoresis in Food Analysis

Encyclopedia of Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 1 - 27

Опубликована: Июнь 24, 2024

Abstract The development and application of faster more powerful analytical methodologies in Food Science is essential to face the demands from regulatory agencies, official laboratories, consumers. In this field, capillary electrophoresis (CE) nowadays a well‐established separation technique for food analysis that possesses exceptional properties related high speed efficiencies combined with small samples reagent requirements. Moreover, it very versatile since different CE modes can be employed broad range applications challenges. fact, should considered as first option when dealing highly polar, charged, or chiral analytes. past two decades, amount research work has been published, demonstrating potential analyze components variety matrices. aim article provide an overview use including summary characteristics main detectors mostly field. Besides, wide view latest (2021–2023) also provided, possibilities technique.

Язык: Английский

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