A validated single-step saliva and serum sample extraction LC-MS/MS method for the analysis of nicotine, cotinine and 3’-hydroxycotinine for clinical vaping studies

Journal of Pharmaceutical and Biomedical Analysis, Год журнала: 2025, Номер unknown, С. 116703 - 116703

Опубликована: Янв. 1, 2025

Язык: Английский

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Green metrics for analytical methodologies: Uncovering sustainable chromatography approaches for detecting emerging contaminants in food and environmental water

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 172, С. 117598 - 117598

Опубликована: Фев. 16, 2024

Язык: Английский

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Computer-assisted multifactorial method development for the streamlined separation and analysis of multicomponent mixtures in (Bio)pharmaceutical settings

Analytica Chimica Acta, Год журнала: 2023, Номер 1293, С. 342178 - 342178

Опубликована: Дек. 27, 2023

Язык: Английский

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Applications of Hydrophilic Interaction and Mixed-mode Liquid Chromatography in Pharmaceutical Analysis

Journal of Chromatography A, Год журнала: 2024, Номер 1739, С. 465524 - 465524

Опубликована: Ноя. 14, 2024

Язык: Английский

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Retention mechanisms of amitriptyline and its impurities on amide, amino, diol, and silica columns in hydrophilic interaction liquid chromatography

Journal of Separation Science, Год журнала: 2024, Номер 47(9-10)

Опубликована: Май 1, 2024

Hydrophilic interaction liquid chromatography (HILIC) has been widely applied to challenging analysis in biomedical and pharmaceutical fields, bridging the gap between normal-phase high-performance reversed-phase (RP-HPLC). This paper comprehensively explores retention mechanisms of amitriptyline its impurities A, B, C, D, F, G on amide, amino, diol, silica columns. Dual HILIC/RP-HPLC were developed, transitional points HILIC RP-HPLC calculated Adsorption partition contributions overall evaluated using Python software regions. The cation exchange mechanism dominates for ionized analytes column (R

Язык: Английский

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Simplified Synthesis of Poly(ethyleneimine)-Modified Silica Particles and Their Application in Oligosaccharide Isolation Methods

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9465 - 9465

Опубликована: Авг. 30, 2024

There are great challenges in the field of natural product isolation and purification pharmacological study oligosaccharide monomers. And these processes still universal problems products (NPs), traditional Chinese medicine (TCM), omics, etc. The same polymer-modified materials designed for special separation oligosaccharides, named Sil-epoxy-PEI Sil-chloropropyl-PEI, were synthesized via two different methods characterized by scanning electron microscopy combined with energy spectrum analysis, Fourier transform infrared spectroscopy, thermogravimetric zeta potential as well surface area Several nucleotide/nucleoside molecules polarities selectivities successfully isolated our laboratory using stainless-steel columns filled material. In addition, saccharide probes oligosaccharides mixtures water extracts Morinda officinalis compared HILIC mode. results showed that resolution separations representative analytes column was higher than Sil-chloropropyl-PEI column, developed stationary phase exhibited improved performance to hydrothermal carbon, amide other previously reported.

Язык: Английский

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Getting Insights Into Chromatographic Properties of HILIС and Mixed‐Mode Homemade Stationary Phases Using Principal Component and Cluster Analyses

Journal of Chemometrics, Год журнала: 2025, Номер 39(3)

Опубликована: Март 1, 2025

ABSTRACT In this work, we compared the chromatographic properties of 27 homemade monomer‐ and polymer‐modified stationary phases synthesized via Ugi reaction for hydrophilic interaction liquid chromatography (HILIC). These along with unmodified substrate were characterized by retention factors 33 polar biologically active compounds belonging to various classes (nucleobases/nucleosides, sugars, carboxylic acids, water‐soluble vitamins). Additionally, widely used Tanaka HILIC test was performed. The experimental data from both characterization approaches processed using several chemometric techniques, including principal component analysis (PCA), hierarchical cluster (HCA), K‐means algorithm. It initially expected that would differ significantly monomer‐modified ones due their mixed‐mode properties. confirmed clear separation these two types on PCA score plot obtained binary logarithms selectivities (calculated all factors). Dissimilarities observed among some resulted in insights into conditions suitable obtaining adsorbents distinct Each class required specific mobile phase composition achieve reasonable characteristics, such as times peak shapes. To exclude long‐lasting re‐equilibration stage associated changes, a smaller set only three proposed, yielding nearly same clustering results complete dataset. This simplified procedure can facilitate rapid newly allow comparison previously studied phases.

Язык: Английский

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Impact of Mobile Phase Composition on Separation Selectivity of Labeled Dextran Ladder in Hydrophilic Interaction Liquid Chromatography

Molecules, Год журнала: 2025, Номер 30(6), С. 1327 - 1327

Опубликована: Март 15, 2025

The glycosylation process plays a crucial role in the structural integrity and biological activity of glycoproteins, where glycans are attached to protein backbone. There many kinds glycans, most common being N-glycans, which can be arranged into three classes, that is, complex, hybrid, high mannoses, forming structurally very diverse set polar compounds difficult detect separate. Most commonly, N-glycans labeled before separation by charged or fluorescence tags for better MS detection, respectively. This study examines influence ionic strength organic modifier selection on fluorescently dextran ladders Hydrophilic Interaction Liquid Chromatography (HILIC). Using Glycan BEH Amide column varying ammonium formate buffer concentration along with acetonitrile methanol ratios, we investigated analyte retention, efficiency, post-column conductivity changes. Our findings reveal changes mobile phase do not contribute selectivity, neither when nor were used as modifiers phase. However, addition significantly mechanism two different prevailing separations mechanisms identified. It was assumed influences folding pattern dextrans around permanent positive charge added tag, selectivity. work presents systematic approach altering composition (buffer concentration, type) control retention selectivity complex glycan analysis. discovery alters behavior provides potential new method refining HILIC compounds.

Язык: Английский

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Preparation of a 1,4-Diazabicyclo[2.2.2]octane Sulfonate Betaine Zwitterionic Stationary Phase and Comparative Evaluation of Its Separation Performance in Hydrophilic Interaction Chromatography

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 344028 - 344028

Опубликована: Апрель 1, 2025

Язык: Английский

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Bioinspired hydrophilic material with parallel ordered channels for simultaneously selective enrichment and rapid separation of glycopeptides and phosphopeptides

Talanta, Год журнала: 2025, Номер 295, С. 128199 - 128199

Опубликована: Апрель 25, 2025

Язык: Английский

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