Light-/pH-Regulated Spiropyran Smart-Responsive Hydrophilic Separation Platform for the Identification of Serum Glycopeptides from Hepatocellular Carcinoma Patients

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

Smart-responsive materials have attracted much attention in the enrichment of post-translational modifications proteins. In this work, for first time, we developed a smart strategy (MNPs-l-DOPA/PEI-SP) based on change hydrophilic properties spiropyran under regulation light and pH to realize controllable release intact glycopeptides. The mechanism possible binding were verified by theoretical calculations. platform MNPs-l-DOPA/PEI-SP was used screen glycoprotein biomarkers hepatocellular carcinoma (HCC) evaluate its cancer diagnostic monitoring performance. A total 3,864 N-glycopeptides containing 166 N-glycoproteins successfully identified serum samples early-stage HCC patients, while 3,266 193 glycoproteins normal control (NC) samples. This work not only provides new ideas efficient glycopeptides with smart-responsive material, but also broadens research possibilities biomarker discovery liquid biopsies.

Язык: Английский

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Nitrogen-modified reduced graphene oxide for serum enrichment of N-glycans and MALDI-TOF MS-based identification of HCC biomarkers

The Analyst, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

N-rGO enriches N -glycans/glycopeptides via HILIC and directly identifies them using MALDI-TOF MS.

Язык: Английский

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Rapid, efficient and highly selective separation and enrichment of glycoprotein by surface-imprinted MOF nanoparticles loaded with high-density boric acid

Separation and Purification Technology, Год журнала: 2024, Номер 354, С. 128911 - 128911

Опубликована: Июль 24, 2024

Язык: Английский

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Fabrication of a polymer brush-functionalized porphyrin-based covalent organic framework for enrichment of N-glycopeptides

Microchimica Acta, Год журнала: 2023, Номер 191(1)

Опубликована: Дек. 13, 2023

Язык: Английский

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Hydrogen peroxide induces the transition of amorphous to crystalline structure of AlOx and enhanced fluoride removal efficiency in coal mine wastewater

Journal of Solid State Chemistry, Год журнала: 2025, Номер 348, С. 125378 - 125378

Опубликована: Апрель 11, 2025

Язык: Английский

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Synthesis of multi-template imprinted mesoporous silica nanoparticles via micelle-based interface imprinting strategy for specific and efficient extraction of five cinnamic acid derivatives from traditional Chinese medicines

Talanta, Год журнала: 2025, Номер 294, С. 128180 - 128180

Опубликована: Апрель 18, 2025

Язык: Английский

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Effectiveness, Surface Treatments, and Modern Trends in the Preparation of Multifunctional Magnetic Nanoparticles for Analytical and Environmental Applications

Royal Society of Chemistry eBooks, Год журнала: 2025, Номер unknown, С. 1 - 29

Опубликована: Май 7, 2025

Applications of multifunctional magnetic nanoparticles (MMNPs) are numerous and include environmental analytical domains. Because their distinct characteristics surface functions, MMNPs useful instruments for separation, biosensing, imaging methods. Their efficacy, treatments, current trends examined in this chapter. With great sensitivity selectivity, excellent methods like resonance (MRI). help solve urgent issues by facilitating pollution identification, water purification, soil remediation applications. Innovative synthesis techniques, including green microfluidic-assisted fabrication, the focus offer scalable sustainable MMNP manufacturing. Furthermore, several related to cost-effectiveness, repeatability, scalability still exist will require further study, as has been mentioned. This chapter emphasizes how important advancement sciences calls more research development area.

Язык: Английский

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Facile preparation of nitrogen/titanium-rich porous organic polymers for specific enrichment ofN-glycopeptides and phosphopeptides

Analytical Methods, Год журнала: 2024, Номер 16(5), С. 695 - 703

Опубликована: Янв. 1, 2024

The comprehensive investigation of protein phosphorylation and glycosylation aids in the discovery novel biomarkers as well understanding pathophysiology illness. In this work, a nitrogen/titanium-rich porous organic polymer was developed by copolymerizing carbohydrazide (CH) 2,3-dihydroxyterephthalaldehyde (2,3-Dha) modifying with Ti4+ (CH-Dha-Ti4+). adequate nitrogen contributes to enrichment glycopeptides via HILIC, while titanium benefits from capturing phosphopeptides through IMAC. proposed method exhibits excellent selectivity (1 : 1000, both for phosphopeptides), LOD (for glycopeptides: 0.05 fmol μL-1, phosphopeptides: 0.2 fmol), loading capacity 100 mg g-1, 125 g-1) size-exclusion effect 10 000, phosphopeptides). Furthermore, CH-Dha-Ti4+ applied capture human serum; 205 45 were detected serum normal controls; 294 63 found uremia patients after being analyzed nano LC-MS/MS. discovered involved several molecular biological processes activities, according gene ontology study.

Язык: Английский

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Mass spectrometry-based structure-specific <?A3B2 pi6?><italic>N-</italic>glycoproteomics and biomedical applications

Acta Biochimica et Biophysica Sinica, Год журнала: 2024, Номер 56(8), С. 1172 - 1183

Опубликована: Июль 26, 2024

linked glycosylation is a common posttranslational modification of proteins that results in macroheterogeneity the site. However, unlike simpler modifications,

Язык: Английский

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Dual-functionalized two-dimensional metal–organic framework composite with highly hydrophilicity for effective enrichment of glycopeptides

Journal of Chromatography B, Год журнала: 2023, Номер 1232, С. 123920 - 123920

Опубликована: Ноя. 15, 2023

Язык: Английский

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