Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 1, 2024
Herbal
medicines
(HMs)
have
long
played
a
pivotal
role
in
preventing
and
treating
various
human
diseases
been
studied
widely.
However,
the
complexities
present
HM
metabolites
their
unclear
mechanisms
of
action
posed
significant
challenges
modernization
traditional
Chinese
medicine
(TCM).
Over
past
two
decades,
mass
spectrometry
imaging
(MSI)
has
garnered
increasing
attention
as
robust
analytical
technique
that
enables
simultaneous
execution
qualitative,
quantitative,
localization
analyses
without
complex
sample
pretreatment.
With
advances
technical
solutions,
MSI
extensively
applied
field
HMs.
MSI,
label-free
ion
can
comprehensively
map
spatial
distribution
plant
native
tissues,
thereby
facilitating
effective
quality
control
Furthermore,
dimension
information
small
molecule
endogenous
within
animal
tissues
provided
by
also
serve
supplement
to
uncover
pharmacological
toxicological
In
review,
we
provide
an
overview
three
most
common
techniques.
addition,
representative
applications
are
highlighted.
Finally,
discuss
current
propose
several
potential
solutions.
We
hope
summary
recent
findings
will
contribute
application
exploring
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 9880 - 9880
Опубликована: Сен. 12, 2024
Mass
spectrometry
(MS)
has
revolutionized
clinical
chemistry,
offering
unparalleled
capabilities
for
biomolecule
analysis.
This
review
explores
the
growing
significance
of
mass
(MS),
particularly
when
coupled
with
liquid
chromatography
(LC),
in
identifying
disease
biomarkers
and
quantifying
biomolecules
diagnostic
prognostic
purposes.
The
unique
advantages
MS
accurately
diverse
molecules
have
positioned
it
as
a
cornerstone
personalized-medicine
advancement.
MS-based
technologies
transformed
precision
medicine,
enabling
comprehensive
understanding
mechanisms
patient-specific
treatment
responses.
LC-MS
shown
exceptional
utility
analyzing
complex
biological
matrices,
while
high-resolution
expanded
analytical
capabilities,
allowing
detection
low-abundance
elucidation
pathways.
integration
other
techniques,
such
ion
mobility
spectrometry,
opened
new
avenues
biomarker
discovery
validation.
As
we
progress
toward
will
be
crucial
addressing
challenges
individualized
patient
care,
driving
innovations
diagnosis,
prognosis,
strategies.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 17, 2025
Alterations
in
amine
metabolite
levels
are
closely
associated
with
the
poor
progression
of
pancreatic
disease,
including
acute
pancreatitis
(AP)
and
cancer
(PC).
However,
effectively
quantifying
visualizing
these
metabolites
through
mass
spectrometry
(MS)
has
proven
to
be
challenging.
Here,
we
have
designed
a
novel
rapid
strategy
for
analyzing
submetabolome
within
liquid
chromatography-mass
(LC-MS)
air-flow-assisted
desorption
electrospray
ionization
imaging
(AFADESI-MSI)
platforms
by
inducing
pair
isotope-labeling-based
photochemical
derivatization
reagents.
The
simultaneous
introduction
4-amino-1-methylpyridinium
moiety
renders
160-
1037-fold
higher
response
MS.
Coupled
full
MS-ddMS2
precursor
ion
scan
modes,
this
labeling
allows
straightforward
detection
423
peaks
indazolone
derivatives
identification
82
biological
samples.
semiquantitation
amines
plasma
from
AP
patients
healthy
controls
resulted
discovery
unreported
aromatic
aminoaldehydes
significant
changes
employing
ethanolamine
distinguishing
severities
early
stage.
In
MSI
platform,
reagent
can
efficiently
derivatize
primary
avoiding
spatial
deviation
significantly
enhancing
sensitivity
rat
brain
kidney.
Further
joint
analysis
pancreas
PC
use
two
allowed
identifying
metabolite,
methylamine.
These
results
together
enhance
role
amine-driven
biomarker
diagnosis
disease
accelerate
application
on-tissue
derivation
MSI.
Revista Brasileira de Criminalística,
Год журнала:
2025,
Номер
14(1), С. 46 - 69
Опубликована: Март 31, 2025
As
análises
forenses
consistem
em
um
conjunto
de
processos
científicos
e
tecnológicos
destinados
a
elucidar
crimes,
fornecendo
suporte
essencial
para
as
investigações
criminais.
evidências
dos
crimes
são
investigadas
laboratórios
criminalística
partir
da
atuação
do
perito
criminal,
por
meio
técnicas
voltadas
áreas
como
toxicologia
genética
forense.
Diante
disso,
esse
trabalho
teve
objetivo
realizar
uma
análise
das
mundialmente
utilizadas
elucidação
bem
analisar
os
relatos
criminais
entre
2004
2024
literatura.
Para
elaboração
pesquisa,
foram
realizadas
buscas
artigos
disponíveis
nas
bases
dados
Scielo,
Science
Direct,
Google
acadêmico,
Europe
PMC
PubMed,
além
sites
órgãos
oficiais
livros,
anos
2024.
Os
mais
frequentes
facilitados
drogas,
sendo
benzodiazepínicos
classe
farmacológica
usada
nestes
crimes.
A
técnica
utilizada
na
grande
maioria
foi
cromatografia
líquida
acoplada
ao
espectrômetro
massas
(CLAE-EM).
Em
geral,
países
desenvolvidos
relatam
forma
completa,
especificando
não
só
aconteceram,
métodos
analíticos
utilizados.
Foi
possível
perceber
falta
detalhamento
casos
ocorridos
no
Brasil,
diferentemente
outros
países.
Por
fim,
concluir
importância
peritos
analíticas
aplicadas
à
área
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 4, 2025
Single-cell
analysis
uncovers
cellular
heterogeneity
and
dynamic
metabolism
at
the
individual
cell
level,
providing
fundamental
insights
into
physiological
pathological
mechanisms
of
life.
Spatial
molecular
mapping
single
cells
requires
advanced
techniques
that
achieve
high
spatial
resolution
comprehensive
coverage
while
preserving
cells'
native
state
with
minimal
preparation.
Here,
we
report
an
ultralow-flow-rate
desorption
electrospray
ionization
mass
spectrometry
imaging
(u-DESI-MSI)
platform
for
in
situ
biomolecule
subcellular
resolution.
The
u-DESI-MSI
single-cell
utilizes
a
stable
ultralow-solvent-flow-rate
(150
nL/min)
system
optimized
geometrical
settings,
avoiding
complicated
hardware
modifications
on
ESI
emitter.
capability
was
demonstrated
by
human
pancreatic
from
different
lineages.
An
unprecedented
achieved
under
rastering
step
size
5
μm,
even
revealing
lipid
distribution
regions.
Using
this
high-spatial-resolution
platform,
effectively
visualized
both
intercellular
intracellular
cells.
Notably,
provides
versatile
tool
direct
their
states
ambient
conditions,
eliminating
need
extensive
sample
We
anticipate
will
facilitate
exploration
heterogeneity,
offering
valuable
variability
metabolism.
Physical Sciences Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 7, 2025
Abstract
Computational
techniques
encompass
all
informatic
tools
that
extract
information
from
large
databases.
Some
remarkable
applications
include
chemoinformatics,
geoinformatics,
and
bioinformatics,
involving
advanced
computing
to
explore
analyse
chemical,
geological
biological
data.
Mass
spectrometry
has
provided
tremendous
input
regarding
useful
instrumentations
enhance
the
relevance
of
computational
methods.
Here,
we
reviewed
methods
for
mass
particularly
OMIC
which
comprises
technologies
measure
some
characteristics
a
family
cellular
molecules,
such
as
genes,
proteins
small
metabolites.
