High-Sensitivity Enzyme-Free Fluorescence Probe Based on CRISPR/Cas13 and the Isothermal Amplification Strategy for Axl Sensing

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Сен. 30, 2024

Axl is an important receptor tyrosine protein kinase that plays a key role in the development and progression of various diseases, such as cancer inflammation. Developing highly sensitive detection method can help improve accuracy, better address-specific clinical needs, guide personalized treatment. In this study, CHA-CRISPR/Cas13 fluorescence probe was established using Axl-specific aptamers mediator to displace polynucleotide chain (TA). Through TA construction, entropy-driven nucleotide catalytic hairpin assembly system created cyclically release RNA activates clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13 activity, triggering its cleavage activity. The activated CRISPR/Cas13 cleaves reporter labeled with BHQ1 FAM at both ends, leading recovery fluorescence. Based on optimization design free energy (△

Язык: Английский

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Target-promoted catalytic DNA molecular circuit network coupled with endonuclease amplification for sensitive and label-free electrochemical aptamer antibiotic assay

Food Control, Год журнала: 2024, Номер 169, С. 111007 - 111007

Опубликована: Ноя. 7, 2024

Язык: Английский

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Target-triggered catalytic hairpin assembly of miR-21/155/1 coupled with dsDNA-reporter amplified detection for prediction of clinically significant coronary artery disease

Sensors and Actuators B Chemical, Год журнала: 2024, Номер unknown, С. 137053 - 137053

Опубликована: Ноя. 1, 2024

Язык: Английский

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DNA Nanotechnology for Application in Targeted Protein Degradation

ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(11), С. 6814 - 6827

Опубликована: Окт. 5, 2024

DNA is a kind of flexible and versatile biomaterial for constructing nanostructures nanodevices. Due to high biocompatibility programmability easy modification fabrication, nanotechnology has emerged as powerful tool application in intracellular targeted protein degradation. In this review, we summarize the recent advances design mechanism degradation technologies such hydrolysis chimeras, lysosomal autophagy based Subsequently, introduce nanotechnologies cascade circuits, nanostructures, dynamic machines. Moreover, present latest developments Finally, vision challenges are discussed.

Язык: Английский

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Combining isolated G-quadruplexes with CHA-3WJ/DNA: A triple signal amplification colorimetric biosensor for detecting Escherichia coli O157: H7

Food Bioscience, Год журнала: 2024, Номер unknown, С. 105303 - 105303

Опубликована: Окт. 1, 2024

Язык: Английский

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Improving Dynamic Control of Toehold-Mediated Strand Displacement Reactions through Phosphorothioate Modifications

Nanoscale, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

The discovery for the first time that phosphorothioate modifications can enhance rate of TMSD in short toehold region. Furthermore, we applied our findings to CHA circuits and DNA probabilistic circuits.

Язык: Английский

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Integrated self-powered sensors based on cubic nanostructure and cascade amplification strategies

Analytica Chimica Acta, Год журнала: 2024, Номер 1335, С. 343446 - 343446

Опубликована: Ноя. 20, 2024

Язык: Английский

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Recent advances in label-free fluorescent DNA sensors

Microchemical Journal, Год журнала: 2024, Номер unknown, С. 112351 - 112351

Опубликована: Дек. 1, 2024

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Cyclometalated Ruthenium (II) Complex-Based Nanoparticles for Enhanced microRNAs Detection and Imaging in Living Cells

Biosensors and Bioelectronics, Год журнала: 2024, Номер unknown, С. 117090 - 117090

Опубликована: Дек. 1, 2024

Язык: Английский

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