Biosensors and Bioelectronics, Год журнала: 2024, Номер 271, С. 117084 - 117084
Опубликована: Дек. 24, 2024
Язык: Английский
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 10, 2025
Diagnosing antimicrobial resistance (AMR) remains critical for improving patient survival rates and treatment outcomes. Current antibiotic susceptibility tests (AST) suffer prolonged turnaround times, necessitating a minimum of 24 h results. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy emerges as promising phenotypic testing method in bacteriology due to its rapid chemical characterization capability. Here, we present an innovative approach utilizing ATR-FTIR AMR assessment, distinguishing between methicillin-resistant Staphylococcus aureus (MRSA) methicillin-susceptible S. (MSSA). Our focuses on detecting early markers effective action using these predict profiles. To identify the earliest time detection, five MSSA MRSA strains were subjected oxacillin exposure up 2 h. We observed discernible molecular changes arising 1 after oxacillin, which absent strains. Bands at 1624 1515 cm-1 identified positive drug response principal component analysis (PCA) associated with peptidoglycan precursor accumulation upon transpeptidation inhibition. develop predictive models determining profiles, implemented ML-based modeling spectral data, reflective oxacillin-induced composition MRSA. Partial least squares discriminant (PLS-DA) support vector machines classification (SVM-C) algorithms produced best results, achieving 100% consistency inhibitory concentration (MIC) classification. independently validated by blind 35 clinical demonstrated agreement profiling determined MIC. study underscores potential accurate capacity revolutionize diagnostics combating resistance.
Язык: Английский
Процитировано
1
Pakistan Journal of Medicine and Dentistry, Год журнала: 2025, Номер 14(1), С. 102 - 108
Опубликована: Янв. 10, 2025
Background: Urinary tract infections (UTIs) are the most common in humans, primarily caused by bacteria, with Escherichia coli (E. coli) responsible for 75-90% of cases. The rise bacterial resistance is largely due to inappropriate antibiotic use, posing significant public health concerns. This study investigated UTI prevalence and inform optimized treatment guidelines. aimed identify pathogens causing UTIs assess their antimicrobial sensitivity commonly used antibiotics. Method: A descriptive cross-sectional was conducted over six months at Khyber Teaching Hospital's Microbiology Department antibiogram profile uropathogens from urine cultures. Urine samples were cultured on CLED media, biochemical tests uropathogens. Antimicrobial susceptibility testing performed isolates using Kirby-Bauer disk diffusion method Mueller Hinton Agar (MHA), following CLSI total 384 patients' collected through a convenient technique processed as per standard data analyzed SPSS-22 shown tables. Results: uropathogen, found 48.17% (n=185) cases, higher females about 48.4% (n=125). Fosfomycin frequently prescribed (31.5%) showed highest (50.2%). Cefotaxime exhibited (38.45%), followed Amoxicillin-clavulanic acid (34.8%). Conclusion: remains dominant pathogen, proving generally effective, while cefotaxime notable. These reports show importance use responsibly. By selecting appropriate antibiotics, we can minimize rate
Язык: Английский
Процитировано
0
Indus journal of bioscience research., Год журнала: 2025, Номер 3(1), С. 559 - 563
Опубликована: Янв. 29, 2025
Background: This medical review examines whether Lady Reading Hospital Peshawar follows IDSA recommendations when obtaining blood and urine cultures before starting antibiotics for new patients coming from ED. The accuracy of culture tests depends on proper specimen collection procedures yet healthcare teams only stick to these methods inconsistently which leads inappropriate antibiotic use raises patient risks. Methodology: Staff reviewed 200 past records through HMIS perform this analysis. Our sample consisted taken 100 alongside tests. examined what extent facilities followed aimed 95% implementation accuracy. research team gathered information about such as age, testing schedule, treatment details. audit uncovered system challenges affecting care doctors' decision structures. Results Discussion: examination revealed poor practices with in 10% cases 2% fell far below the desired rate. busy ED environment made clinicians rush processing samples led reduced adherence protocols increased risk developing drug-resistant bacteria. Conclusion: shows does not follow guidelines by taking diagnostic treatment. Clinicians need education workflow changes plus real-time monitoring meet rules help get better results.
Язык: Английский
Процитировано
0
Journal of Xenobiotics, Год журнала: 2025, Номер 15(2), С. 43 - 43
Опубликована: Март 11, 2025
Over the years, abuse of antibiotics has increased, leading to their presence in environment. Therefore, a sustainable method for detecting these substances is crucial. Researchers have explored biomass-based carbon dots (CDs) detect various contaminants, due low cost, environmental friendliness, and support circular economy. In our study, we reported synthesis CDs using pinecones (PCs) pinebark (PB) through microwave method. We characterized PCCDs PBCDs X-ray diffraction, Raman spectroscopy, Transmission Electron Microscope, Fourier transform infrared, Ultraviolet-visible, photoluminescence (PL) spectroscopy. The were tested detection amoxicillin (AMX) tetracycline (TC). results indicated that sizes 19.2 nm 18.39 nm, respectively, confirmed 002 plane graphitic structure. They exhibited excitation wavelength dependence, good stability, quantum yields ranging from 6% 11%. demonstrated “turn-off” TC AMX. limits (LOD) across broader concentration range found be 0.062 µM 0.2237 PBCDs. For AMX detection, presented an LOD 0.49 µM.
Язык: Английский
Процитировано
0
Sustainable Environment, Год журнала: 2025, Номер 11(1)
Опубликована: Апрель 16, 2025
Язык: Английский
Процитировано
0
Talanta, Год журнала: 2025, Номер 295, С. 128388 - 128388
Опубликована: Май 25, 2025
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9893 - 9893
Опубликована: Сен. 13, 2024
The rapid resistance developed by pathogenic microorganisms against the current antimicrobial pool represents a serious global public health problem, leading to search for new antibiotic agents. scorpion
Язык: Английский
Процитировано
2
Cureus, Год журнала: 2024, Номер unknown
Опубликована: Июнь 1, 2024
Microbial detection and antimicrobial resistance (AMR) surveillance are critical components of public health efforts to combat infectious diseases preserve the efficacy agents. While foundational in microbial identification, traditional cultural methods often laborious, time-consuming, limited their ability detect AMR markers. In response these challenges, innovative paradigms have emerged, leveraging advances molecular biology, genomics, proteomics, nanotechnology, bioinformatics. This comprehensive review provides an overview approaches beyond for surveillance. Molecular-based techniques such as polymerase chain reaction (PCR) next-generation sequencing (NGS) offer enhanced sensitivity specificity, enabling rapid identification pathogens determinants. Mass spectrometry-based provide accurate biomarkers, including matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) biosensor technologies. Nanotechnology approaches, nanoparticle-based assays nanopore sequencing, novel platforms sensitive label-free Embracing holds immense promise improving disease diagnosis, antibiotic stewardship, containment efforts. However, challenges cost, standardization, integration with existing healthcare systems must be addressed realize full potential By fostering interdisciplinary collaboration innovation, we can strengthen our detect, monitor, AMR, safeguarding generations.
Язык: Английский
Процитировано
1
Sensors and Actuators B Chemical, Год журнала: 2024, Номер 424, С. 136866 - 136866
Опубликована: Окт. 30, 2024
Язык: Английский
Процитировано
1
Microorganisms, Год журнала: 2024, Номер 12(11), С. 2121 - 2121
Опубликована: Окт. 23, 2024
Antimicrobial resistance (AMR) has long been framed as an epidemiological and public health concern. Its impacts on the environment are unclear. Yet, basis for AMR is altered cell physiology. Just this affects how microbes interact with antimicrobials, it can also affect they their own species, other non-living environment. Moreover, if globally notorious causing landscape-level environmental issues, then these effects could alter biodiversity ecosystem function a grand scale. To investigate possibilities, we compiled peer-reviewed literature from past 20 years regarding in toxic freshwater cyanobacterial harmful algal blooms (HABs). We examined evidence of affecting HAB frequency, severity, or persistence. Although no study within our scope was explicitly designed to address question, multiple studies reported AMR-associated changes HAB-forming cyanobacteria (and co-occurring microbes) that pertained directly timing, toxicity, phase, well dynamics HAB-afflicted aquatic food webs. These findings highlight potential have far-reaching (including loss function) bring into focus importance confronting complex interrelated issues such HABs concert, interdisciplinary tools perspectives.
Язык: Английский
Процитировано
0