Опубликована: Янв. 1, 2024
Язык: Английский
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
The Single-probe single-cell mass spectrometry (SCMS) is an innovative analytical technique designed for metabolomic profiling, offering a miniaturized, multifunctional device capable of direct coupling to spectrometers. It ambient leveraging microscale sampling and nanoelectrospray ionization (nanoESI), enabling the analysis cells in their native environments without need extensive sample preparation. Due its miniaturized design versatility, this allows applications diverse research areas, including metabolomics, quantification target molecules single cell, MS imaging (MSI) tissue sections, investigation extracellular live spheroids. This review explores recent advancements Single-probe-based techniques applications, emphasizing potential utility advancing methodologies bioanalysis.
Язык: Английский
Процитировано
1
Food Chemistry, Год журнала: 2025, Номер 473, С. 143069 - 143069
Опубликована: Янв. 27, 2025
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Chinese Medicine, Год журнала: 2025, Номер 20(1)
Опубликована: Май 12, 2025
Abstract Mass spectrometry (MS)-based metabolomics has emerged as a transformative tool to unraveling components and their mechanisms in traditional Chinese medicine (TCM). The integration of advanced analytical platforms, such LC–MS GC–MS, coupled with metabolomics, propelled the qualitative quantitative characterization TCM’s complex components. This review comprehensively examines applications MS-based elucidating TCM efficacy, spanning chemical composition analysis, molecular target identification, mechanism-of-action studies, syndrome differentiation. Recent innovations functional spatial single-cell metabolic flux analysis have further expanded research horizons. Artificial intelligence (AI) bioinformatics offer promising avenues for overcoming bottlenecks, enhancing database standardization, driving interdisciplinary breakthroughs. However, challenges remain, including need improved data processing expansion, understanding metabolite-gene-protein interactions. By addressing these gaps, can bridge practices modern biomedical research, fostering global acceptance TCM. highlights synergy MS techniques, computational tools, holistic philosophy, presenting forward-looking perspective on its clinical translation internationalization.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0