CRISPR-Cas-based colorimetric strategies for nucleic acids detection DOI

Y Yang,

Xingyu Li, Xiaobo Wang

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 118058 - 118058

Опубликована: Ноя. 1, 2024

Язык: Английский

A Review on Optical Biosensors for Monitoring of Uric Acid and Blood Glucose Using Portable POCT Devices: Status, Challenges, and Future Horizons DOI Creative Commons
Kermue Vasco Jarnda, Heng Dai, Anwar Ali

и другие.

Biosensors, Год журнала: 2025, Номер 15(4), С. 222 - 222

Опубликована: Март 31, 2025

The growing demand for real-time, non-invasive, and cost-effective health monitoring has driven significant advancements in portable point-of-care testing (POCT) devices. Among these, optical biosensors have emerged as promising tools the detection of critical biomarkers such uric acid (UA) blood glucose. Different transduction methods, like fluorescence, surface plasmon resonance (SPR), colorimetric approaches, are talked about, with a focus on how sensitive, specific, they are. Despite considerable advancements, several challenges persist, including sensor stability, miniaturization, interference effects, need calibration-free operation. This review also explores issues related to cost-effectiveness, data integration, wireless connectivity remote monitoring. further examines regulatory considerations commercialization aspects biosensors, addressing gap between research developments clinical implementation. Future perspectives emphasize integration artificial intelligence (AI) healthcare improved diagnostics, alongside development wearable implantable continuous Innovative potential change way people manage their by quickly accurately measuring glucose levels. is especially true decentralized solutions grows. By critically evaluating existing work exploring limitations opportunities field, this will help guide more efficient, accessible, reliable POCT devices that can improve patient outcomes quality life.

Язык: Английский

Процитировано

1

CRISPR/Cas-powered “sweet” DNA nano-sponge for highly sensitive detection of pathogen nucleic acid with portable personal glucometer DOI

Huiling You,

Jingwen Shan,

Yang Bai

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160618 - 160618

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

A smartphone-based colorimetric sensing platform for detection of blood viscosity and hemoglobin DOI

Dawei Feng,

Shan Gao,

J. Grace Liu

и другие.

Sensors and Actuators B Chemical, Год журнала: 2025, Номер 432, С. 137495 - 137495

Опубликована: Фев. 24, 2025

Язык: Английский

Процитировано

0

MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands DOI Creative Commons
Francesca Miceli,

Sara Bracaglia,

Daniela Sorrentino

и другие.

Nucleic Acids Research, Год журнала: 2025, Номер 53(6)

Опубликована: Март 20, 2025

Abstract Here we report on the development of a CRISPR-based assay for sensitive and specific detection antibodies antigens directly in complex sample matrices. The assay, called Molecular Assay based antibody-Induced Guide-RNA Enzymatic Transcription (MAIGRET), is use responsive synthetic DNA template that triggers cell-free vitro transcription guide RNA strand upon recognition target antibody. Such transcribed activates collateral activity Cas12a enzyme, leading to downstream cleavage fluorophore/quencher-labeled reporter thus resulting an increase measured fluorescence signal. We have used MAIGRET six different with high sensitivity (detection limit picomolar range) specificity (no signal presence non-target antibodies). can also be adapted competitive approach antigens. With MAIGRET, significantly expand scope applicability sensing approaches potentially enable measurement any molecular which antibody available.

Язык: Английский

Процитировано

0

Construction of Biomimetic Nanochannel, Property Regulation, and Biomarker Detection DOI
Peizhi Li, Jian Zhao, Dan Liang

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Abstract The significance of biomimetic nanochannel in the field biosensors is gaining increasing recognition. controllable construction nanochannels and their performance modulation have demonstrated great importance obtained wide interest. offer high sensitivity, enabling sensors to swiftly identify target biomarkers complex biological samples, with detection limits reaching picomolar level. Furthermore, they demonstrate exceptional selectivity reproducibility, making them ideal tools for biomarker detection. In recent years, utilizing shown remarkable detecting a range biomarkers. This review aims explore opportunities challenges associated technology biosensor applications, focusing on these nanochannels, as well applications nucleic acids, proteins, organisms, small molecules. Providing forward‐looking insights into this cutting‐edge aspired, particular emphasis technological advancements, addressing current challenges, discussing future trends.

Язык: Английский

Процитировано

0

CRISPR Digital Sensing: From Micronano-Collaborative Chip to Biomolecular Detection DOI
Xinyi Wang, Longjie Zhong, Wenbin Zhang

и другие.

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Май 24, 2025

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) sensing technology proved to be valuable during the COVID-19 pandemic through its sensitivity, specificity, robustness, and versatility. However, issues such as overreliance on amplification, susceptibility false positives, lack of quantification strategies, complex operation procedures have hindered broader application in bioanalysis clinical diagnostics. collision between micronano-collaborative chips CRISPR has effectively addressed these bottlenecks, offering innovative solutions for diagnosis treatment. Unlike conventional micronano chips, digital enhance CRISPR's response trace amounts target molecules by leveraging highly controllable local environments compartmentalized microreactors. This advancement improves detection efficiency revolutionizes traditional vitro bioanalytical processes. First, working principles, fabrication techniques, performance metrics CRISPR-based droplet microfluidics microarray are examined. Then, applications bioassays reviewed, emphasizing their importance advancing systems gene editing. Finally, prospects explored, particularly potential body surface biomonitoring development opportunities biomedical field.

Язык: Английский

Процитировано

0

Commercial Strip‐Inspired One‐Pot CRISPR‐Based Chip for Multiplexed Detection of Respiratory Viruses DOI
Hong Zhang, Xiaolin Hu,

Xudong Bao

и другие.

Small Methods, Год журнала: 2024, Номер 9(1)

Опубликована: Сен. 19, 2024

The absence of sensitive, multiplexed, and point-of-care assays poses a critical obstacle in promptly responding to emerging human respiratory virus (HRV) pandemics. Herein, RECOGNIZER (re-building commercial pregnancy strips via large-size nanoflowers), an innovative one-pot CRISPR assay, is presented that employs commercially available identify several types HRVs. superiority the assay mainly relies on two aspects: (i) DNA nanoflowers possessing high surface-to-volume ratio well-defined surface allow for considerable probe loading density minimized non-specific interaction, achieving impressive signal-to-noise proportion exceeding tenfold at 1 nM target. (ii) design reaction, multi-channel chip, custom-made app enables rapid, sample-to-answer, multiplexed analysis four HRVs 25 min. This demonstrates sensitivity 5.42 pM synthetic SARS-CoV-2 RNA 10 copies µL-1 plasmids after pre-amplification. Finally, proposed approach indicated 100% accuracy 50 clinical swab samples, demonstrating robust performance distinguishing from other versatility scalability renders it user-friendly platform infection monitoring, offering significant potential improving pandemic response efforts.

Язык: Английский

Процитировано

2

Advancements of Prokaryotic Argonautes in Molecular Diagnostics and Further Perspectives DOI
Yuting Shang, Xiang Gao,

Hao-Zhao Wei

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 118122 - 118122

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

2

CRISPR-Cas-based colorimetric strategies for nucleic acids detection DOI

Y Yang,

Xingyu Li, Xiaobo Wang

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 118058 - 118058

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

1