Tuberculosis
(TB)
was,
until
SARS-CoV-2
pandemic,
the
leading
cause
of
death
by
a
single
infectious
agent
contaminating
over
10.6
million
people
with
1.6
deaths
in
2021
worldwide.
Herein,
we
present
proof-of-principle
strategy
for
detecting
recombinant
protein
CFP10:ESAT6
using
an
impedimetric
immunosensor,
which
could
aid
diagnosis
tuberculosis.
The
immunosensor
was
developed
indium
tin
oxide
electrodes
modified
3-aminopropyltrimethoxysilane
monolayer
to
covalently
immobilize
anti-CFP10
antibodies.
interaction
antibody
recognition
platform
directly
monitored
and
measured
cyclic
voltammetry
electrochemical
impedance
spectroscopy,
respectively.
After
analytical
features
optimization,
Langmuir
isotherm
response
from
0.5
ng
mL
-1
50
pCFP10:ESAT6,
limit
detection
4.80
quantification
15.97
were
achieved,
4-hour
assay
time.
Selectivity
tests
conducted
presence
DENV
NS1
Spike
proteins
at
concentration
20
,
is
one-tenth
used
optimize
pCFP10,
indicate
that
selective
pCFP10:ESAT6.
Additionally,
repeatability
reproducibility
confirm
suitable,
accurate,
protein.
small
sample
volume
required,
short
testing
time
underscore
remarkable
capabilities
this
its
potential
point-of-care
screening
diagnostic
applications.
Digital Discovery,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
AI-integrated
electrochemical
sensors
boost
peak
resolution
and
sensitivity,
enabling
precise
detection
of
electroactive
species
in
complex
matrices.
This
method
enhances
analytical
capabilities,
providing
an
analytically
robust
solution.
Biosensors,
Год журнала:
2025,
Номер
15(4), С. 231 - 231
Опубликована: Апрель 5, 2025
Disease
diagnosis
is
not
only
related
to
individual
health
but
also
a
crucial
part
of
public
prevention.
Electrochemical
biosensors
combine
the
high
sensitivity
electrochemical
methods
with
inherent
selectivity
biological
components,
offering
advantages
such
as
excellent
sensitivity,
fast
response
time,
and
low
cost.
The
generated
electrical
signals
have
linear
relationship
target
analyte,
allowing
for
identification
concentration
detection.
This
has
become
very
attractive
technology.
review
offers
summary
recent
advancements
in
biosensor
research
disease
China.
It
systematically
categorizes
summarizes
developed
China
detecting
cancer,
infectious
diseases,
inflammation,
neurodegenerative
disorders.
Additionally,
delves
into
fundamental
working
principles,
classifications,
materials,
preparation
techniques,
other
critical
aspects
biosensors.
Finally,
it
addresses
key
challenges
impeding
advancement
examines
promising
future
directions
their
development.
Biosensors,
Год журнала:
2024,
Номер
14(10), С. 496 - 496
Опубликована: Окт. 12, 2024
This
review
examines
recent
advancements
in
electrochemical
immunosensors
for
the
detection
of
organophosphate
pesticides,
focusing
on
strategies
to
enhance
sensitivity
and
selectivity.
The
widespread
use
these
pesticides
has
necessitated
development
rapid,
accurate,
field-deployable
methods.
We
discuss
fundamental
principles
explore
innovative
approaches
improve
their
performance.
These
include
utilization
nanomaterials
such
as
metal
nanoparticles,
carbon
nanotubes,
graphene
signal
amplification;
enzyme-based
amplification
strategies;
design
three-dimensional
electrode
architectures.
integration
sensors
into
microfluidic
lab-on-a-chip
devices
enabled
miniaturization
automation,
while
screen-printed
disposable
electrodes
have
facilitated
on-site
testing.
analyze
challenges
faced
real
sample
analysis,
including
matrix
effects
stability
biological
recognition
elements.
Emerging
trends
application
artificial
intelligence
data
interpretation
aptamer-based
are
highlighted.
also
considers
potential
commercialization
hurdles
that
must
be
overcome
adoption.
Future
research
directions
identified,
multi-analyte
platforms
with
emerging
technologies
like
Internet
Things.
comprehensive
overview
provides
insights
current
state
field
outlines
promising
avenues
future
pesticide
detection.
Electrochemical
sensors
exhibit
distinct
advantages
over
alternative
sensor
modalities
in
the
swift
detection
of
microorganisms.
In
this
study,
we
devised
a
label-free
electrochemical
immunosensor
utilizing
CoFe-MOFs@MWCNTs
composite-modified
electrodes
for
specific
Salmonella
milk.
The
integration
composites
onto
electrode
surface
effectively
enhanced
current
response
and
furnished
an
abundance
active
sites,
subsequently
augmented
by
electrodeposited
AuNPs
signal
amplification
antibody
conjugation.
microstructural
characteristics
composite
were
meticulously
examined
via
scanning
electron
microscopy,
while
its
elemental
composition
chemical
structure
thoroughly
analyzed
using
energy-dispersive
X-ray
spectroscopy
Fourier
transform
infrared
spectroscopy,
respectively.
Upon
optimization
experimental
parameters,
demonstrated
exceptional
sensitivity,
specificity,
stability,
exhibiting
linear
range
spanning
from
1.04×104
to
1.04×108
CFU
mL-1
Salmonella,
with
limit
(LOD)
2.89
×
103
mL-1.
This
innovative
holds
promise
cow's
milk
presents
novel
approach
monitoring
foodborne
pathogens.
Foods,
Год журнала:
2025,
Номер
14(2), С. 182 - 182
Опубликована: Янв. 9, 2025
The
widespread
use
of
thiamethoxam
has
led
to
pesticide
residues
that
have
sparked
global
concerns
regarding
ecological
and
human
health
risks.
A
pressing
requirement
exists
for
a
detection
method
is
both
swift
sensitive.
Herein,
we
introduced
an
innovative
fluorescence
biosensor
constructed
from
alendronic
acid
(ADA)-modified
upconversion
nanoparticles
(UCNPs)
linked
with
magnetic
(MNPs)
via
aptamer
recognition
the
thiamethoxam.
Through
base
pairing,
thiamethoxam-specific
aptamer-functionalized
MNPs
(apt-MNPs)
were
integrated
complementary
DNA-functionalized
UCNPs
(cDNA-UCNPs)
create
MNPs@UCNPs
biosensor.
Thiamethoxam
specifically
attached
apt-MNPs,
leading
their
separation
cDNA-UCNPs,
which
in
turn
reduction
intensity
at
544
nm
following
by
external
field.
change
(ΔI)
was
directly
correlated
concentration
thiamethoxam,
enabling
quantitative
analysis
pesticide.
With
optimized
parameters,
capable
quantifying
within
span
0.4-102.4
ng·mL-1,
it
achieved
limit
as
minute
0.08
ng·mL-1.
Moreover,
leveraging
properties
MNPs,
assay
duration
could
be
abbreviated
25
min.
research
exhibited
precise
sensing
platform
yielded
promising
results
samples
cucumber,
cabbage,
apple.
Heliyon,
Год журнала:
2025,
Номер
11(4), С. e42782 - e42782
Опубликована: Фев. 1, 2025
Per-
and
Poly-fluoroalkyl
substances
(PFASs)
are
among
the
that
have
been
widely
employed
across
world
due
to
their
distinct
features.
These
chemicals'
great
stability
in
environment
capacity
be
released
from
consumer
goods
demonstrated
existence
all
matrices.
Additionally,
world's
attention
has
drawn
these
substances'
direct
relation
human
health
recent
years.
This
research
aimed
unravel
nexus
of
PFASs
with
obesity
endocrine
disruption
as
a
comprehensive
review.
Studies
shown
drinking
water
is
primary
way
which
humans
exposed
PFASs.
As
result,
it
become
difficult
determine
how
contaminated
compounds
impacts
affect
health.
Through
various
routes,
metabolic
processes,
possible
effects
on
hypothalamus-pituitary-thyroid
axis,
chemicals
increase
incidence
obesity,
particularly
during
crucial
growth
phases.
Another
negative
impact
glands'
function,
for
controlling
body's
physiological
functions.
leads
alterations
hormone
production.
The
exposure
include
secondary
hyperparathyroidism,
non-alcoholic
fatty
liver,
diabetes,
cardiovascular
illnesses,
reproductive
abnormalities,
infertility.
Because
characteristics,
including
propensity
propagate
through
food
chain,
accumulate
biomagnify,
ultimately
pose
threat
life,
replace
remove
chemicals.
