Tuberculosis
(TB)
was,
until
SARS-CoV-2
pandemic,
the
leading
cause
of
death
by
a
single
infectious
agent
contaminating
over
10.6
million
people
with
1.6
deaths
in
2021
worldwide.
Herein,
we
present
proof-of-principle
strategy
for
detecting
recombinant
protein
CFP10:ESAT6
using
an
impedimetric
immunosensor,
which
could
aid
diagnosis
tuberculosis.
The
immunosensor
was
developed
indium
tin
oxide
electrodes
modified
3-aminopropyltrimethoxysilane
monolayer
to
covalently
immobilize
anti-CFP10
antibodies.
interaction
antibody
recognition
platform
directly
monitored
and
measured
cyclic
voltammetry
electrochemical
impedance
spectroscopy,
respectively.
After
analytical
features
optimization,
Langmuir
isotherm
response
from
0.5
ng
mL
-1
50
pCFP10:ESAT6,
limit
detection
4.80
quantification
15.97
were
achieved,
4-hour
assay
time.
Selectivity
tests
conducted
presence
DENV
NS1
Spike
proteins
at
concentration
20
,
is
one-tenth
used
optimize
pCFP10,
indicate
that
selective
pCFP10:ESAT6.
Additionally,
repeatability
reproducibility
confirm
suitable,
accurate,
protein.
small
sample
volume
required,
short
testing
time
underscore
remarkable
capabilities
this
its
potential
point-of-care
screening
diagnostic
applications.
Micromachines,
Год журнала:
2025,
Номер
16(3), С. 280 - 280
Опубликована: Фев. 27, 2025
Exosomes
are
important
biomarkers
for
liquid
biopsy
in
early
cancer
screening
which
play
roles
many
biological
processes,
including
apoptosis,
inflammatory
response,
and
tumor
metastasis.
In
this
study,
an
electrochemical
aptamer
immunosensor
based
on
Au/MXene
AuPtPdCu
was
constructed
the
sensitive
detection
of
colorectal
cancer-derived
exosomes.
AuNPs
were
deposited
situ
surface
MXenes
as
a
sensing
platform
due
to
their
large
specific
area,
excellent
conductivity,
higher
number
active
sites
immobilization.
The
CD63
immobilized
can
specifically
capture
target
Therefore,
AuPtPdCu-Apt
nanoprobe
further
enhanced
sensitivity
accuracy
immunosensor.
A
low
limit
19
particles
μL−1
achieved
linear
range
50
5
×
104
under
optimal
conditions.
developed
herein
showed
satisfactory
stability
anti-interference
ability
exosomes
real
serum
samples.
Foods,
Год журнала:
2025,
Номер
14(5), С. 854 - 854
Опубликована: Март 2, 2025
The
binding
interactions
between
okadaic
acid
(OA)
aptamers
and
OA
molecules
are
crucial
for
developing
effective
detection
methods.
This
study
aims
to
identify
the
recognition
site
establish
a
reliable
protocol
through
computational
simulations
experimental
validations.
After
determining
target
sequence
(OA-2),
molecular
docking
using
Sybyl-X
H-dock
were
conducted
predict
affinity
interaction
sites
of
with
their
targets.
These
predictions
subsequently
validated
experiments
based
on
Förster
resonance
energy
transfer
(FRET)
principle.
combined
approach
not
only
confirmed
predictions,
identifying
"major
region"
as
basis
OA-2,
but
also
provided
deeper
insights
into
mechanisms.
Subsequently,
classical
AuNPs-aptamer
colorimetric
method
was
established
OA-2
applied
real
shellfish
samples,
achieving
limit
quantification
(LOQ)
5.0
μg
kg-1.
recoveries
in
spiked
samples
ranged
from
79.0%
122.9%,
relative
standard
deviation
(RSD)
less
than
14.7%.
results
this
contribute
development
robust
methods
aptamer-target
interactions,
enhancing
potential
practical
applications
toxin
monitoring.
Toxins,
Год журнала:
2025,
Номер
17(4), С. 165 - 165
Опубликована: Март 26, 2025
Aflatoxin
M1
(AFM1)
appears
in
the
milk
of
animals
that
have
consumed
feed
contaminated
with
aflatoxin
B1.
AFM1
presence
is
regulated
by
European
Commission,
which
has
set
maximum
allowable
limits
for
adult
and
infant
consumption
to
50
25
pg/mL,
respectively.
Here,
a
rapid
sensitive
method
detecting
based
on
an
immersible
silicon
photonic
chip
presented.
The
features
two
U-shaped
nitride
waveguides
formed
as
Mach–Zehnder
interferometers.
One
interferometer
functionalized
AFM1–bovine
serum
albumin
conjugate
other
BSA
serve
blank.
connected
broad-band
white
LED
spectrophotometer
bifurcated
optical
fiber
assay
performed
immersing
mixture
anti-AFM1
antibody.
Then,
sequentially
immersed
biotinylated
anti-rabbit
IgG
antibody
streptavidin
solutions
signal
enhancement.
completed
20
min
detection
limit
undiluted
pg/mL.
Given
its
analytical
performance
absence
pumps
fluidics
lead
compact
instrument
design,
proposed
immunosensor
ideal
on-site
samples.
Foods,
Год журнала:
2025,
Номер
14(7), С. 1174 - 1174
Опубликована: Март 27, 2025
Dinotefuran
and
clothianidin
belong
to
the
third
generation
of
nicotinic
insecticides
are
widely
used
in
crop
pest
control.
It
is
necessary
detect
their
residues
food.
The
time-resolved
fluorescent
microspheres
lateral
flow
immunoassay
(TRFMs-LFIA)
has
advantages
high
sensitivity,
short
duration,
simple
operation
suitable
for
rapid
field
testing.
In
this
study,
two
haptens
(FCA-1,
FCA-2)
were
synthesized
three
steps
conjugated
carrier
proteins
obtain
artificial
antigens,
which
subsequently
monoclonal
antibody
preparation.
A
TRFMs-LFIA
based
on
antibodies
was
established
dinotefuran
limit
detection
(LOD)
0.045
ng/mL,
with
an
IC50
0.61
ng/mL
a
linear
range
(IC20~IC80)
0.12~3.11
ng/mL.
LOD
0.11
0.94
0.24~3.65
Cross-reactivity
rates
seven
tested
structural
analogs
less
than
1.5%.
pretreatment
method
optimized
wheat,
cucumber,
cabbage
samples,
time-saving
(20
min)
easy
operate.
average
recovery
ranged
from
88.0%
114.8%,
corresponding
coefficients
variation
appearing
(CV)
between
1.9%
13.5%.
results
actual
samples
detected
by
consistent
those
Ultra-Performance
Liquid
Chromatography
coupled
Tandem
Mass
Spectrometry
(UPLC-MS/MS).
These
demonstrate
that
sensitive,
accurate,
rapid,
real
sample
detection.
Technologies,
Год журнала:
2025,
Номер
13(4), С. 138 - 138
Опубликована: Апрель 2, 2025
Nucleic
acid
amplification
(NAA)
is
a
technique
that
increases
the
number
of
copies
gene,
making
it
possible
to
detect
microorganisms.
This
often
used
in
clinical
tests,
biochemical
analysis,
and
environmental
assays,
mention
only
few.
However,
developing
portable,
robust,
low-cost
measurement
platforms
evaluate
NAA
products
remains
technological
challenge.
Therefore,
this
work,
we
introduce
an
attractive
unit
for
detecting
quantifying
nucleic
acids
based
on
capacitively
coupled
contactless
conductivity
detection
(C4D)
principle.
The
proposed
unit,
ReC4D,
combines
electrical
resonance
with
C4D
enhance
sensitivity
when
evaluating
reaction.
ReC4D
units
advantages
are
twofold:
(i)
transducer
electrically
isolated
allow
its
reuse,
(ii)
induced
minimizes
stray
capacitances
conventional
which
enhances
sensitivity,
linear
operating
range,
improves
limit
(LoD).
Furthermore,
evaluated
device
different
concentrations
SARS-CoV-2
genetic
material
compared
measurements
from
unit.
Thus,
demonstrate
can
measure
LoD
0.24
copyμL
5.618
kHzlog(copyμL).
These
results
position
close
state-of-the-art
testing
platforms,
added
value
low
cost,
robustness,
reusability,
affordability.
Nanomaterials,
Год журнала:
2025,
Номер
15(8), С. 612 - 612
Опубликована: Апрель 16, 2025
Simple
and
accurate
analysis
of
cancer-related
biomarkers
is
very
important
for
disease
screening
auxiliary
diagnosis.
This
study
proposed
a
facile
immunoassay
that
used
gold
nanostar-labeled
rabbit
anti-AFP
as
capture
antibody
nanoparticle-conjugated
goat
anti-rabbit
IgG
an
enhance
the
construction
detection
strategy
AFP
analysis.
Investigations
indicated
50
nm
diameter
GNS-labeled
can
specifically
catch
AFPs
by
direct
profile
or
further
signal
amplification
through
AuNP-tagged
combination.
Results
showed
developed
method
holds
8.6
ng/mL
sensitivity,
20.0–110.0
range,
acceptable
precision
fine
accuracy,
well
favorable
specificity.
application
to
real
serum
determination
are
highly
related
those
ECLIA
(correlation
coefficient
0.931).
The
has
simple-operation
merit
suitable
clinical
large-scale
samples
cancers.
