Exploring electrochemical impedance spectroscopy for the early diagnosis of Mycobacterium tuberculosis using CFP10:ESAT6 protein detection DOI Creative Commons

Letícia Pamela Garcia Ribeiro,

Juliana Cancino‐Bernardi, Claudia A. Razzino

и другие.

Frontiers in Sensors, Год журнала: 2024, Номер 5

Опубликована: Дек. 24, 2024

Tuberculosis (TB) was, until SARS-CoV-2 pandemic, the leading cause of death by a single infectious agent contaminating over 10.6 million people with 1.6 deaths in 2021 worldwide. Herein, we present proof-of-principle strategy for detecting recombinant protein CFP10:ESAT6 using an impedimetric immunosensor, which could aid diagnosis tuberculosis. The immunosensor was developed indium tin oxide electrodes modified 3-aminopropyltrimethoxysilane monolayer to covalently immobilize anti-CFP10 antibodies. interaction antibody recognition platform directly monitored and measured cyclic voltammetry electrochemical impedance spectroscopy, respectively. After analytical features optimization, Langmuir isotherm response from 0.5 ng mL -1 50 pCFP10:ESAT6, limit detection 4.80 quantification 15.97 were achieved, 4-hour assay time. Selectivity tests conducted presence DENV NS1 Spike proteins at concentration 20 , is one-tenth used optimize pCFP10, indicate that selective pCFP10:ESAT6. Additionally, repeatability reproducibility confirm suitable, accurate, protein. small sample volume required, short testing time underscore remarkable capabilities this its potential point-of-care screening diagnostic applications.

Язык: Английский

An Electrochemical Immunosensor for Sensitive Detection of Exosomes Based on Au/MXenes and AuPtPdCu DOI Creative Commons
Jie Gao, Rong Yang,

Xiaorui Zhu

и другие.

Micromachines, Год журнала: 2025, Номер 16(3), С. 280 - 280

Опубликована: Фев. 27, 2025

Exosomes are important biomarkers for liquid biopsy in early cancer screening which play roles many biological processes, including apoptosis, inflammatory response, and tumor metastasis. In this study, an electrochemical aptamer immunosensor based on Au/MXene AuPtPdCu was constructed the sensitive detection of colorectal cancer-derived exosomes. AuNPs were deposited situ surface MXenes as a sensing platform due to their large specific area, excellent conductivity, higher number active sites immobilization. The CD63 immobilized can specifically capture target Therefore, AuPtPdCu-Apt nanoprobe further enhanced sensitivity accuracy immunosensor. A low limit 19 particles μL−1 achieved linear range 50 5 × 104 under optimal conditions. developed herein showed satisfactory stability anti-interference ability exosomes real serum samples.

Язык: Английский

Процитировано

0

Establishing Detection Methods for Okadaic Acid Aptamer–Target Interactions: Insights from Computational and Experimental Approaches DOI Creative Commons
Wenchong Shan,

Jiefang Sun,

Runqing Liu

и другие.

Foods, Год журнала: 2025, Номер 14(5), С. 854 - 854

Опубликована: Март 2, 2025

The binding interactions between okadaic acid (OA) aptamers and OA molecules are crucial for developing effective detection methods. This study aims to identify the recognition site establish a reliable protocol through computational simulations experimental validations. After determining target sequence (OA-2), molecular docking using Sybyl-X H-dock were conducted predict affinity interaction sites of with their targets. These predictions subsequently validated experiments based on Förster resonance energy transfer (FRET) principle. combined approach not only confirmed predictions, identifying "major region" as basis OA-2, but also provided deeper insights into mechanisms. Subsequently, classical AuNPs-aptamer colorimetric method was established OA-2 applied real shellfish samples, achieving limit quantification (LOQ) 5.0 μg kg-1. recoveries in spiked samples ranged from 79.0% 122.9%, relative standard deviation (RSD) less than 14.7%. results this contribute development robust methods aptamer-target interactions, enhancing potential practical applications toxin monitoring.

Язык: Английский

Процитировано

0

Label-free electrochemical immunosensor for the accurate detection of α-synuclein based on Au/SiO2/Si electrode DOI
Nasrin Siraj Lopa, Mohammad Karbalaei Akbari,

Yanbin Cui

и другие.

Journal of Industrial and Engineering Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Rapid detection of Salmonella in milk by a label-free electrochemical immunosensor based on CoFe-MOFs@MWCNTs modified electrode DOI
Li Huo, Shi‐Wen Huang,

Torbjörn G.I. Ling

и другие.

International Dairy Journal, Год журнала: 2025, Номер unknown, С. 106242 - 106242

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Nitrogen-Doped Graphene Quantum Dots (N-GQDs): A Promising Material for the Development of Electrochemical Immunosensors DOI Creative Commons
Gustavo Martins,

Ana Laura S. Galvan,

Márcia Gabriela Pianaro Valenga

и другие.

ACS Applied Nano Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Язык: Английский

Процитировано

0

Aflatoxin M1 Determination in Whole Milk with Immersible Silicon Photonic Immunosensor DOI Creative Commons

Dimitra Kourti,

Μichailia Angelopoulou,

Eleni Makarona

и другие.

Toxins, Год журнала: 2025, Номер 17(4), С. 165 - 165

Опубликована: Март 26, 2025

Aflatoxin M1 (AFM1) appears in the milk of animals that have consumed feed contaminated with aflatoxin B1. AFM1 presence is regulated by European Commission, which has set maximum allowable limits for adult and infant consumption to 50 25 pg/mL, respectively. Here, a rapid sensitive method detecting based on an immersible silicon photonic chip presented. The features two U-shaped nitride waveguides formed as Mach–Zehnder interferometers. One interferometer functionalized AFM1–bovine serum albumin conjugate other BSA serve blank. connected broad-band white LED spectrophotometer bifurcated optical fiber assay performed immersing mixture anti-AFM1 antibody. Then, sequentially immersed biotinylated anti-rabbit IgG antibody streptavidin solutions signal enhancement. completed 20 min detection limit undiluted pg/mL. Given its analytical performance absence pumps fluidics lead compact instrument design, proposed immunosensor ideal on-site samples.

Язык: Английский

Процитировано

0

A Monoclonal Antibody-Based Time-Resolved Fluorescence Microsphere Lateral Flow Immunoassay for Dinotefuran and Clothianidin Detection DOI Creative Commons
Ling Qin, Haojie Chen,

Yingxiang Nie

и другие.

Foods, Год журнала: 2025, Номер 14(7), С. 1174 - 1174

Опубликована: Март 27, 2025

Dinotefuran and clothianidin belong to the third generation of nicotinic insecticides are widely used in crop pest control. It is necessary detect their residues food. The time-resolved fluorescent microspheres lateral flow immunoassay (TRFMs-LFIA) has advantages high sensitivity, short duration, simple operation suitable for rapid field testing. In this study, two haptens (FCA-1, FCA-2) were synthesized three steps conjugated carrier proteins obtain artificial antigens, which subsequently monoclonal antibody preparation. A TRFMs-LFIA based on antibodies was established dinotefuran limit detection (LOD) 0.045 ng/mL, with an IC50 0.61 ng/mL a linear range (IC20~IC80) 0.12~3.11 ng/mL. LOD 0.11 0.94 0.24~3.65 Cross-reactivity rates seven tested structural analogs less than 1.5%. pretreatment method optimized wheat, cucumber, cabbage samples, time-saving (20 min) easy operate. average recovery ranged from 88.0% 114.8%, corresponding coefficients variation appearing (CV) between 1.9% 13.5%. results actual samples detected by consistent those Ultra-Performance Liquid Chromatography coupled Tandem Mass Spectrometry (UPLC-MS/MS). These demonstrate that sensitive, accurate, rapid, real sample detection.

Язык: Английский

Процитировано

0

Resonance-Induced Capacitively Coupled Contactless Conductivity Detection (ReC4D) Unit for Nucleic Acid Amplification Testing DOI Creative Commons
Roberto G. Ramírez-Chavarría, Jorge A. Uc-Martín, Bryan E. Alvarez-Serna

и другие.

Technologies, Год журнала: 2025, Номер 13(4), С. 138 - 138

Опубликована: Апрель 2, 2025

Nucleic acid amplification (NAA) is a technique that increases the number of copies gene, making it possible to detect microorganisms. This often used in clinical tests, biochemical analysis, and environmental assays, mention only few. However, developing portable, robust, low-cost measurement platforms evaluate NAA products remains technological challenge. Therefore, this work, we introduce an attractive unit for detecting quantifying nucleic acids based on capacitively coupled contactless conductivity detection (C4D) principle. The proposed unit, ReC4D, combines electrical resonance with C4D enhance sensitivity when evaluating reaction. ReC4D units advantages are twofold: (i) transducer electrically isolated allow its reuse, (ii) induced minimizes stray capacitances conventional which enhances sensitivity, linear operating range, improves limit (LoD). Furthermore, evaluated device different concentrations SARS-CoV-2 genetic material compared measurements from unit. Thus, demonstrate can measure LoD 0.24 copyμL 5.618 kHzlog(copyμL). These results position close state-of-the-art testing platforms, added value low cost, robustness, reusability, affordability.

Язык: Английский

Процитировано

0

Facile Immunoassay Constructed by Gold Nanostar-Labeled Rabbit-AFP Antibody and Gold Nanoparticle-Conjugated Goat Anti-Rabbit IgG DOI Creative Commons
Kang Yang, Fang Yang, Xiaoling Lü

и другие.

Nanomaterials, Год журнала: 2025, Номер 15(8), С. 612 - 612

Опубликована: Апрель 16, 2025

Simple and accurate analysis of cancer-related biomarkers is very important for disease screening auxiliary diagnosis. This study proposed a facile immunoassay that used gold nanostar-labeled rabbit anti-AFP as capture antibody nanoparticle-conjugated goat anti-rabbit IgG an enhance the construction detection strategy AFP analysis. Investigations indicated 50 nm diameter GNS-labeled can specifically catch AFPs by direct profile or further signal amplification through AuNP-tagged combination. Results showed developed method holds 8.6 ng/mL sensitivity, 20.0–110.0 range, acceptable precision fine accuracy, well favorable specificity. application to real serum determination are highly related those ECLIA (correlation coefficient 0.931). The has simple-operation merit suitable clinical large-scale samples cancers.

Язык: Английский

Процитировано

0

Recent advances in MOF-modified electrochemical sensor for point-of-care detecting cardiac biomarkers DOI
Mohamed Ahmed, Jingyi Bai, Lingxue Kong

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118277 - 118277

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0