Analysis of Bacterial Metabolites in Breath Gas of Critically Ill Patients for Diagnosis of Ventilator-Associated Pneumonia—A Proof of Concept Study DOI Creative Commons
Wojciech Filipiak, Robert Włodarski, Karolina Żuchowska

и другие.

Biomolecules, Год журнала: 2024, Номер 14(12), С. 1480 - 1480

Опубликована: Ноя. 21, 2024

Bacterial infection of the lower respiratory tract frequently occurs in mechanically ventilated patients and may develop into life-threatening conditions. Yet, existing diagnostic methods have moderate sensitivity specificity, which results overuse broad-spectrum antibiotics administered prophylactically. This study aims to evaluate suitability volatile bacterial metabolites for breath-based test, is used diagnosing Ventilator-Associated Pneumonia (VAP). The vitro experiments with pathogenic bacteria most prevalent VAP etiology (i.e., Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa) were performed identify bacteria-derived using a specially designed cultivation system enabling headspace sampling GC-MS analysis. Thirty-nine compounds found be significantly metabolized by tested species and, therefore, selected monitoring exhaled breath critically ill, (MV) patients. emission volatiles from medical devices was investigated estimate risk spoiling exogenous pollutants. then evaluated differentiate non-infected MV controls Receiver Operating Characteristic (ROC) analysis, AUC, sensitivity, specificity calculated. Nine that passed verification through non-parametric ANOVA test significance LASSO penalization identified as key discriminators between non-VAP model achieved an AUC 0.893, values 87% 82.4%, respectively, being competitive traditional methods. Further validation could solidify its clinical utility critical care settings.

Язык: Английский

Integrated Smart Mass Spectrometry Platform Enables Volatilomics Based Breath Biopsy DOI Creative Commons

Shangzhewen Li,

Zhihui Yu,

Zhengnan Cen

и другие.

Green Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 100284 - 100284

Опубликована: Июнь 1, 2025

Язык: Английский

Процитировано

0
Analysis of Bacterial Metabolites in Breath Gas of Critically Ill Patients for Diagnosis of Ventilator-Associated Pneumonia—A Proof of Concept Study DOI Creative Commons
Wojciech Filipiak, Robert Włodarski, Karolina Żuchowska

и другие.

Biomolecules, Год журнала: 2024, Номер 14(12), С. 1480 - 1480

Опубликована: Ноя. 21, 2024

Bacterial infection of the lower respiratory tract frequently occurs in mechanically ventilated patients and may develop into life-threatening conditions. Yet, existing diagnostic methods have moderate sensitivity specificity, which results overuse broad-spectrum antibiotics administered prophylactically. This study aims to evaluate suitability volatile bacterial metabolites for breath-based test, is used diagnosing Ventilator-Associated Pneumonia (VAP). The vitro experiments with pathogenic bacteria most prevalent VAP etiology (i.e., Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa) were performed identify bacteria-derived using a specially designed cultivation system enabling headspace sampling GC-MS analysis. Thirty-nine compounds found be significantly metabolized by tested species and, therefore, selected monitoring exhaled breath critically ill, (MV) patients. emission volatiles from medical devices was investigated estimate risk spoiling exogenous pollutants. then evaluated differentiate non-infected MV controls Receiver Operating Characteristic (ROC) analysis, AUC, sensitivity, specificity calculated. Nine that passed verification through non-parametric ANOVA test significance LASSO penalization identified as key discriminators between non-VAP model achieved an AUC 0.893, values 87% 82.4%, respectively, being competitive traditional methods. Further validation could solidify its clinical utility critical care settings.

Язык: Английский

Процитировано

1