Decoding Microbial Plastic Colonisation: Multi‐Omic Insights Into the Fast‐Evolving Dynamics of Early‐Stage Biofilms DOI Creative Commons
Charlotte E. Lee, Lauren F. Messer,

Ruddy Wattiez

и другие.

PROTEOMICS, Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

Marine plastispheres represent dynamic microhabitats where microorganisms colonise plastic debris and interact. Metaproteomics has provided novel insights into the metabolic processes within these communities; however, early interactions driving plastisphere formation remain unclear. This study utilised metaproteomic metagenomic approaches to explore on low-density polyethylene (LDPE) over 3 (D3) 7 (D7) days, focusing microbial diversity, activity biofilm development. In total, 2948 proteins were analysed, revealing dominant proteomes from Pseudomonas Marinomonas, with near-complete metagenome-assembled genomes (MAGs). dominated at D3, whilst D7, along Acinetobacter, Vibrio other genera became more prevalent. Marinomonas showed high expression of reactive oxygen species (ROS) suppression proteins, associated oxidative stress regulation, granule formation, alternative carbon utilisation enzymes, also indicated nutrient limitations. Interestingly, 13 alkanes xenobiotic degradation enzymes expressed by five genera. The toxins, several type VI secretion system (TVISS) their competitive advantage against taxa. Upregulated pathways relating substrate transport suggested enhanced cross-feeding diverse community. These enhance our understanding ecology its potential for biotechnological applications.

Язык: Английский

Decoding Microbial Plastic Colonisation: Multi‐Omic Insights Into the Fast‐Evolving Dynamics of Early‐Stage Biofilms DOI Creative Commons
Charlotte E. Lee, Lauren F. Messer,

Ruddy Wattiez

и другие.

PROTEOMICS, Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

Marine plastispheres represent dynamic microhabitats where microorganisms colonise plastic debris and interact. Metaproteomics has provided novel insights into the metabolic processes within these communities; however, early interactions driving plastisphere formation remain unclear. This study utilised metaproteomic metagenomic approaches to explore on low-density polyethylene (LDPE) over 3 (D3) 7 (D7) days, focusing microbial diversity, activity biofilm development. In total, 2948 proteins were analysed, revealing dominant proteomes from Pseudomonas Marinomonas, with near-complete metagenome-assembled genomes (MAGs). dominated at D3, whilst D7, along Acinetobacter, Vibrio other genera became more prevalent. Marinomonas showed high expression of reactive oxygen species (ROS) suppression proteins, associated oxidative stress regulation, granule formation, alternative carbon utilisation enzymes, also indicated nutrient limitations. Interestingly, 13 alkanes xenobiotic degradation enzymes expressed by five genera. The toxins, several type VI secretion system (TVISS) their competitive advantage against taxa. Upregulated pathways relating substrate transport suggested enhanced cross-feeding diverse community. These enhance our understanding ecology its potential for biotechnological applications.

Язык: Английский

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