Computers in Biology and Medicine, Год журнала: 2024, Номер 184, С. 109396 - 109396
Опубликована: Ноя. 16, 2024
Язык: Английский
Journal of Cancer, Год журнала: 2025, Номер 16(6), С. 1860 - 1872
Опубликована: Фев. 28, 2025
Background: Breast cancer is the most prevalent malignancy among females worldwide. Extensive research has highlighted stem cells (CSCs) as critical drivers of tumor initiation, progression, recurrence, and therapy resistance. However, heterogeneity breast (BCSCs) their dynamic roles within microenvironment remain inadequately understood. Methods: This study utilized single-cell RNA sequencing dataset to categorize BCSCs into two subgroups investigate pseudo-time developmental dynamics. Bulk transcriptomic data from TCGA-BRCA were integrated assess prognostic significance infiltration abundance BCSCs-2 subgroup. Functional enrichment, co-expression network analysis, somatic mutation profiling performed elucidate key biological pathways genetic features. Additionally, drug sensitivity analyses conducted using Connectivity Map database identify potential therapeutic strategies. Results: A total 459 identified further classified distinct subpopulations: BCSCs-1 BCSCs-2. High was associated with poor prognosis an immunosuppressive microenvironment. Co-expression analysis 16 genes linked BCSCs-2, while revealed patterns its infiltration. Drug suggested that patients high could benefit classical chemotherapy agents, such Cisplatin, other novel compounds. Conclusions: offers insights functional in cancer. The findings highlight importance subgroup, providing biomarkers targets for precision medicine management.
Язык: Английский
Процитировано
1
Molecular Oncology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 10, 2025
The dynamics of focal adhesions (FAs) are essential physiological processes involved in cell spreading, metastasis, and regulation the actin cytoskeleton. FAs complex structures comprising proteins, such as paxillin zyxin, which interact with extracellular membranes influence motility morphology. Although related studies have been reported various cancers, function molecular mechanisms oral squamous carcinoma (OSCC) remain unknown. We investigated coordination between cytoskeleton FA specifically introducing pituitary tumor‐transforming gene 1 ( PTTG1 ; also known regulator sister chromatid separation, securin) into OSCC. Furthermore, we explored co‐localization several through small interfering RNA (siRNA) control or siRNA‐vasodilator‐stimulated phosphoprotein VASP ) ‐ , examining mediated by induced changes OSCC both vitro vivo . knockdown regulates dynamic cytoskeleton, restricting protrusion from front to rear cells. Our findings may provide new insights how cells each other on surface OSCC, influencing metastatic properties.
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2720 - 2720
Опубликована: Март 18, 2025
Though not specifically designed for cancer therapy, several FDA-approved drugs such as metformin, aspirin, and simvastatin have an effect in lowering the incidence of cancer. However, there is a great discrepancy between vitro concentrations needed to eliminate cells plasma concentration normally tolerated within body. At present, no universal explanation this mechanisms been proposed including targeting stem (CSCs) or cellular senescence. CSCs are with ability self-renewal differentiation known be resistant chemotherapy. Senescence response damage stress, characterized by permanent cell-cycle arrest apoptotic resistance. Although, both situations, few examples where low were most effective, satisfactory data support that either senescence target these drugs. In review, we concisely summarize used non-cancer conditions well their potential action incidence. addition, propose prolonged low-dose administration (PLDA) specific can useful effectively preventing metastasis formation selected patients.
Язык: Английский
Процитировано
0
Medical Oncology, Год журнала: 2025, Номер 42(6)
Опубликована: Май 19, 2025
Язык: Английский
Процитировано
0
Translational Oncology, Год журнала: 2024, Номер 44, С. 101916 - 101916
Опубликована: Март 20, 2024
Metastasis of hepatocellular carcinoma (HCC) critically impacts the survival prognosis patients, with pivotal role stem cells in initiating invasive metastatic behaviors. The Flap Endonuclease 1 (FEN1) is delineated as a metallonuclease, quintessential for myriad cellular processes including DNA replication, synthesis, damage rectification, Okazaki fragment maturation, baseexcision repair, and preservation genomic stability. Furthermore, it has been recognized an oncogene diverse range malignancies. Our antecedent research highlighted pronounced overexpression protein FEN1 carcinoma, where amplifies invasiveness potential liver cancer cells. However, its precise (LCSCs) remains enigma requires further investigation.
Язык: Английский
Процитировано
2
Computers in Biology and Medicine, Год журнала: 2024, Номер 184, С. 109396 - 109396
Опубликована: Ноя. 16, 2024
Язык: Английский
Процитировано
1