Nano TransMed, Год журнала: 2025, Номер unknown, С. 100080 - 100080
Опубликована: Март 1, 2025
Язык: Английский
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 25, 2025
Язык: Английский
Процитировано
0
Journal of Controlled Release, Год журнала: 2025, Номер 380, С. 269 - 282
Опубликована: Фев. 6, 2025
Язык: Английский
Процитировано
0
Journal of Virology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 3, 2025
ABSTRACT Virion formation and egress are sophisticated processes that rely on the spatial temporal organization of host cell membranes manipulation machineries involved in protein sorting, membrane bending, fusion, fission. These result infectious virions, defective particles, various vesicle-like structures. In herpes simplex virus 1 (HSV-1) infections, virions capsid-less known as light (L)-particles, formed. HSV-1 infection also stimulates release particles resemble extracellular vesicles (EVs). productively infected cells, most EVs generated through CD63 tetraspanin biogenesis pathway lack viral components. A smaller subset EVs, endosomal sorting complexes required for transport (ESCRT) pathway, contains both factors. Viral mechanisms tightly regulate EV biogenesis, including inhibition autophagy—a process critical increased production CD63+ during infection. Mutant viruses fail to suppress autophagy instead promote microvesicle from plasma membrane. Additionally, ICP0 (Infected Cell Protein 0) enhances The different types can be separated by density gradients due their distinct biophysical properties. L-particles ESCRT+ display a pro-viral role, supporting replication, whereas exhibit antiviral effects. Overall, these studies highlight yields numerous diverse with type composition shaped ability evade responses. likely shape microenvironment determine disease outcomes.
Язык: Английский
Процитировано
0
Pathogens and Immunity, Год журнала: 2025, Номер 10(1), С. 140 - 158
Опубликована: Фев. 14, 2025
The science of extracellular vesicles (EVs) is a rapidly growing field that spans multiple aspects normal physiology and pathophysiology. EVs play critical role in most basic biological processes cell-cell communications under conditions disease. have “gone viral” not only terms research popularity, but also our realization they exhibit an elaborate crosstalk with viruses, particularly the enveloped ones, which are released by cells as part their virulence cycle yet replicative. Here, we highlight some complexities underlying EV-virus pathways provide insights on key challenges from viewpoint EV biology.
Язык: Английский
Процитировано
0
Clinical Reviews in Allergy & Immunology, Год журнала: 2025, Номер 68(1)
Опубликована: Фев. 20, 2025
Язык: Английский
Процитировано
0
Pakistan BioMedical Journal, Год журнала: 2025, Номер unknown, С. 01 - 01
Опубликована: Фев. 28, 2025
Mesenchymal stem cells, also known as MSCs, are pluripotent cells originating from embryonic mesoderm that can develop into a range of cell types and self-renew. Because this property, MSCs an effective treatment for various diseases. widely distributed in the body be obtained bone marrow, umbilical cord, cord blood, embryo, adipose tissue, dental pulp, skeletal muscle, amniotic fluid, skin, other tissues. Among these, human cord-based easy to collect isolate, have more immunomodulatory function proliferation potential than natural, non-invasive, do not raise ethical questions. They exhibit minimal immunity proliferate readily vitro. These attracted attention many researchers due their powerful tools treating Extensive research studies on clinical applications MSC-based therapies including cancer diseases, heart, neurological, orthopedic disorders been carried out recent years. However, compared intact MSC-derived exosomes offer advantages when used therapeutic agents. First all, application prevents transfer DNA damage or mutations. Second, small (30-100 nm diameter) move easily, while too big through capillaries, most them don't make it past first pass capillary bed. Third, unlike whole transported stored at low temperatures lengthy periods time without losing bioactivity. Furthermore, lipid bilayer walls protect content molecules' bioactivity complicated physiological environment. Fourth, quantitatively applied patients clinic improve outcomes engineered exogenous exosome modification endogenous bioengineering acquire specific properties ((Hu et al, 2022; Dilsiz, 2024). Therapeutic deliverable emerging viable cargo delivery vehicles because natural intercellular communication, great biocompatibility, immunogenicity, toxicity, blood circulation ability, aptitude traverse biological barriers. Since membrane-bound nanoparticles, they usually create lower immune system reaction cells. This makes biocompatible enhances possibility therapy will successful. Exosomes easily made cultured longer period optimal conditions. possible produce high quantities use treat Based preclinical research, 39 trials now investigating (available online: http://www.clinicaltrials.gov/) some which published results.
Язык: Английский
Процитировано
0
Life, Год журнала: 2025, Номер 15(3), С. 410 - 410
Опубликована: Март 6, 2025
Adipose tissue is responsible for fat storage and an important producer of extracellular vesicles (EVs). The biological content exosomes, one kind EV, provides information on aspects such as immunometabolic alterations. This study aimed to compare three plasma exosome isolation methods—using a commercial kit (CK), size exclusion chromatography (SEC), differential centrifugation (DC)—and select the best one. Individuals categorized by normal high body percentages were used. DC CK proven be most advantageous out methods, so we suggest these methods further protein molecular analyses, respectively. Still, emphasize importance selecting appropriate methodology depending specific research objectives. At same time, no statistical differences in quality, morphology, total protein, or microRNA concentration observed between individuals percentage, that exosomal cargo varies with percentages.
Язык: Английский
Процитировано
0
Artificial Cells Nanomedicine and Biotechnology, Год журнала: 2025, Номер 53(1), С. 122 - 137
Опубликована: Март 10, 2025
The signaling mechanisms active within mesenchymal stromal cells (MSCs) influence the composition of microvesicles (MVs) and exosomes (Exos) secreted by them. Previously, we showed that priming MSCs with a p38 pharmacological inhibitor (pMSCs) rejuvenates them improves their ability to promote ex vivo hematopoietic stem cell (HSC) expansion. This study examined whether pMSCs exerted HSC-supportive via MVs (pMVs) Exos (pExos). Our findings demonstrate distinct regulation HSC fate pMVs pExos. promoted expansion long-term HSCs (LT-HSCs), distinguished robust self-renewal capacity superior engraftment ability. In contrast, pExos facilitated short-term (ST-HSCs) high proliferative differentiation potential. Infusing combination pMVs- pExos-expanded as composite graft resulted in significantly higher engraftment, emphasizing synergistic interaction between LT- ST-HSC populations. Gene expression studies, functional phenotypic experiments regulate quiescence Egr1/Cdkn1a axis, while control proliferation Nfya/Cdkn1a axis. These provide insights into molecular underlying differential function It also proposes strategy using "MSC-derived biologics" for improving transplantation success.
Язык: Английский
Процитировано
0
Cancers, Год журнала: 2025, Номер 17(6), С. 940 - 940
Опубликована: Март 10, 2025
Exosomes have emerged as pivotal players in precision oncology, offering innovative solutions to longstanding challenges such metastasis, therapeutic resistance, and immune evasion. These nanoscale extracellular vesicles facilitate intercellular communication by transferring bioactive molecules that mirror the biological state of their parent cells, positioning them transformative tools for cancer diagnostics therapeutics. Recent advancements exosome engineering, artificial intelligence (AI)-driven analytics, isolation technologies are breaking barriers scalability, reproducibility, clinical application. Bioengineered exosomes being leveraged CRISPR-Cas9 delivery, while AI models enhancing biomarker discovery liquid biopsy accuracy. Despite these advancements, key obstacles heterogeneity populations lack standardized protocols persist. This review synthesizes pioneering research on biology, molecular translation, emphasizing dual roles both mediators tumor progression intervention. It also explores emerging areas, including microbiome–exosome interactions integration machine learning exosome-based medicine. By bridging innovation with translational strategies, this work charts a forward-looking path integrating into next-generation care, setting it apart comprehensive guide overcoming technological hurdles rapidly evolving field.
Язык: Английский
Процитировано
0
Genes, Год журнала: 2025, Номер 16(3), С. 330 - 330
Опубликована: Март 12, 2025
Extracellular vesicles (EVs) are nanovesicles that facilitate intercellular communication by carrying essential biomolecules under physiological and pathological conditions including microRNAs (miRNAs). They found in various body fluids, such as blood, urine, saliva, their levels fluctuate with disease progression, making them valuable diagnostic tools. However, isolating EVs is challenging due to small size biological complexity. Here, we summarize the principles behind most common EV isolation methods ultracentrifugation, precipitation, immunoaffinity, sorting, ultrafiltration, exclusion chromatography, microfluidics while highlighting protocol strengths weaknesses. We also review main strategies identify quantify circulating miRNAs a particular focus on EV-encapsulated miRNAs. Since these hold special clinical interest derived from superior stability therapeutic potential, information provided here should provide guidance for future research initiatives promising field of treatment based
Язык: Английский
Процитировано
0