Arthritis Research & Therapy,
Год журнала:
2025,
Номер
27(1)
Опубликована: Март 10, 2025
Takayasu
arteritis
(TAK)
is
an
inflammatory
vasculitis
that
affects
the
aorta
and
its
primary
branches.
The
pathogenesis
of
TAK
remains
elusive,
yet
identifying
key
cell
types
in
patients
crucial
for
uncovering
cellular
heterogeneity
discovering
potential
therapeutic
targets.
This
study
utilized
single-cell
transcriptome
analysis
on
aortic
specimens
from
three
patients,
with
control
data
sourced
a
publicly
available
database
(GSE155468).
Additionally,
bulk
RNA
sequencing
was
performed
peripheral
CD4
+
CD8
T
cells
eight
matched
healthy
volunteers.
All
participants
were
recruited
at
Anzhen
Hospital,
Capital
Medical
University,
China,
between
January
2020
December
2023.
Single-cell
identified
11
predominant
tissues,
notable
differences
proportions
controls.
cells,
B
macrophages,
smooth
muscle
(SMCs),
fibroblasts
exhibited
subtype-specific
gene
expression
signatures,
changes
interactions
monocyte-macrophages,
highlighting
their
active
involvement
TAK.
Bulk
RNA-Seq
blood
showed
upregulation
complement
system
genes,
underscoring
significance
signaling
pathway
TAK's
immunopathogenesis.
findings
underscore
various
immune
structural
tissues
reveal
presence
cells.
These
insights
are
instrumental
novel
targets
developing
robust
disease
monitoring
methods
Chromoblastomycosis
(CBM)
is
a
chronic
neglected
fungal
disease
that
causes
serious
damage
to
the
physical
and
mental
health
of
patients.
5-Aminolevulinic
acid
photodynamic
therapy
(ALA-PDT)
has
garnered
significant
attention
in
recent
era
for
treatment
CBM
exhibited
promising
effects
several
clinical
case
reports.
We
established
mice
footpad
infection
model
with
Fonsecaea
monophora
analyzed
impact
PDT
on
immune
response
macrophages
using
single-cell
sequencing.
The
results
showed
mouse
skin
F.
an
increase
inflammatory
cells,
primarily
mononuclear-macrophages,
activation
complement
enhancement
cell
chemotaxis,
leading
upregulation
anti-infection-related
pathways.
After
ALA-PDT
treatment,
number
cells
decreased,
while
upregulated
expression
antigen-recognition-related
genes,
enhancing
phagocytosis
autophagy-related
biological
functions.
Arthritis Research & Therapy,
Год журнала:
2025,
Номер
27(1)
Опубликована: Март 10, 2025
Takayasu
arteritis
(TAK)
is
an
inflammatory
vasculitis
that
affects
the
aorta
and
its
primary
branches.
The
pathogenesis
of
TAK
remains
elusive,
yet
identifying
key
cell
types
in
patients
crucial
for
uncovering
cellular
heterogeneity
discovering
potential
therapeutic
targets.
This
study
utilized
single-cell
transcriptome
analysis
on
aortic
specimens
from
three
patients,
with
control
data
sourced
a
publicly
available
database
(GSE155468).
Additionally,
bulk
RNA
sequencing
was
performed
peripheral
CD4
+
CD8
T
cells
eight
matched
healthy
volunteers.
All
participants
were
recruited
at
Anzhen
Hospital,
Capital
Medical
University,
China,
between
January
2020
December
2023.
Single-cell
identified
11
predominant
tissues,
notable
differences
proportions
controls.
cells,
B
macrophages,
smooth
muscle
(SMCs),
fibroblasts
exhibited
subtype-specific
gene
expression
signatures,
changes
interactions
monocyte-macrophages,
highlighting
their
active
involvement
TAK.
Bulk
RNA-Seq
blood
showed
upregulation
complement
system
genes,
underscoring
significance
signaling
pathway
TAK's
immunopathogenesis.
findings
underscore
various
immune
structural
tissues
reveal
presence
cells.
These
insights
are
instrumental
novel
targets
developing
robust
disease
monitoring
methods
