Single-cell transcriptome analysis reveals cellular heterogeneity in the aortas of Takayasu arteritis DOI Creative Commons
Na Gao, He Tang, Taotao Li

и другие.

Arthritis Research & Therapy, Год журнала: 2025, Номер 27(1)

Опубликована: Март 10, 2025

Takayasu arteritis (TAK) is an inflammatory vasculitis that affects the aorta and its primary branches. The pathogenesis of TAK remains elusive, yet identifying key cell types in patients crucial for uncovering cellular heterogeneity discovering potential therapeutic targets. This study utilized single-cell transcriptome analysis on aortic specimens from three patients, with control data sourced a publicly available database (GSE155468). Additionally, bulk RNA sequencing was performed peripheral CD4 + CD8 T cells eight matched healthy volunteers. All participants were recruited at Anzhen Hospital, Capital Medical University, China, between January 2020 December 2023. Single-cell identified 11 predominant tissues, notable differences proportions controls. cells, B macrophages, smooth muscle (SMCs), fibroblasts exhibited subtype-specific gene expression signatures, changes interactions monocyte-macrophages, highlighting their active involvement TAK. Bulk RNA-Seq blood showed upregulation complement system genes, underscoring significance signaling pathway TAK's immunopathogenesis. findings underscore various immune structural tissues reveal presence cells. These insights are instrumental novel targets developing robust disease monitoring methods

Язык: Английский

Single-cell sequencing of PIT1-positive pituitary adenoma highlights the pro-tumour microenvironment mediated by IFN-γ-induced tumour-associated fibroblasts remodelling DOI

Liang Lyu,

Yong Jiang, Weichao Ma

и другие.

British Journal of Cancer, Год журнала: 2023, Номер 128(6), С. 1117 - 1133

Опубликована: Янв. 11, 2023

Язык: Английский

Процитировано

20

Transcriptome Analysis Reveals Dynamic Microglial-Induced A1 Astrocyte Reactivity via C3/C3aR/NF-κB Signaling After Ischemic Stroke DOI
Song Wang,

Yuhualei Pan,

Chengjie Zhang

и другие.

Molecular Neurobiology, Год журнала: 2024, Номер 61(12), С. 10246 - 10270

Опубликована: Май 7, 2024

Язык: Английский

Процитировано

9

High order expression dependencies finely resolve cryptic states and subtypes in single cell data DOI Creative Commons
Abel Jansma, Yuelin Yao, Jareth C. Wolfe

и другие.

Molecular Systems Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Язык: Английский

Процитировано

1

Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing DOI Creative Commons

Wenyi Chen,

Xuelin Wu,

Muhammad Danish Yaqoob

и другие.

Open Medicine, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 1, 2025

Chromoblastomycosis (CBM) is a chronic neglected fungal disease that causes serious damage to the physical and mental health of patients. 5-Aminolevulinic acid photodynamic therapy (ALA-PDT) has garnered significant attention in recent era for treatment CBM exhibited promising effects several clinical case reports. We established mice footpad infection model with Fonsecaea monophora analyzed impact PDT on immune response macrophages using single-cell sequencing. The results showed mouse skin F. an increase inflammatory cells, primarily mononuclear-macrophages, activation complement enhancement cell chemotaxis, leading upregulation anti-infection-related pathways. After ALA-PDT treatment, number cells decreased, while upregulated expression antigen-recognition-related genes, enhancing phagocytosis autophagy-related biological functions.

Язык: Английский

Процитировано

1

Single-cell transcriptome analysis reveals cellular heterogeneity in the aortas of Takayasu arteritis DOI Creative Commons
Na Gao, He Tang, Taotao Li

и другие.

Arthritis Research & Therapy, Год журнала: 2025, Номер 27(1)

Опубликована: Март 10, 2025

Takayasu arteritis (TAK) is an inflammatory vasculitis that affects the aorta and its primary branches. The pathogenesis of TAK remains elusive, yet identifying key cell types in patients crucial for uncovering cellular heterogeneity discovering potential therapeutic targets. This study utilized single-cell transcriptome analysis on aortic specimens from three patients, with control data sourced a publicly available database (GSE155468). Additionally, bulk RNA sequencing was performed peripheral CD4 + CD8 T cells eight matched healthy volunteers. All participants were recruited at Anzhen Hospital, Capital Medical University, China, between January 2020 December 2023. Single-cell identified 11 predominant tissues, notable differences proportions controls. cells, B macrophages, smooth muscle (SMCs), fibroblasts exhibited subtype-specific gene expression signatures, changes interactions monocyte-macrophages, highlighting their active involvement TAK. Bulk RNA-Seq blood showed upregulation complement system genes, underscoring significance signaling pathway TAK's immunopathogenesis. findings underscore various immune structural tissues reveal presence cells. These insights are instrumental novel targets developing robust disease monitoring methods

Язык: Английский

Процитировано

1