Replication stress marker phospho-RPA2 predicts response to platinum and PARP inhibitors in homologous recombination-proficient ovarian cancer DOI Open Access
Angela M. Schab,

Amanda Compadre,

Richard Drexler

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

ABSTRACT Background Ovarian cancer treatment includes cytoreductive surgery, platinum-based chemotherapy, and often poly (ADP-ribose) polymerase (PARP) inhibitors. Homologous recombination (HR)-deficiency is a well-established predictor of therapy sensitivity. However, over 50% HR-proficient tumors also exhibit sensitivity to standard-of-care treatments. Currently, there are no biomarkers identify which will be sensitive therapy. Replication stress may serve as key determinant response. Methods We evaluated phospho-RPA2-T21 (pRPA2) foci via immunofluorescence potential biomarker replication in formalin-fixed, paraffin-embedded tumor samples collected at diagnosis from patients treated with platinum chemotherapy (discovery cohort: n = 31, validation 244) or PARP inhibitors (n 87). Recurrent 37) were analyzed. pRPA2 scores calculated using automated imaging analysis. Samples defined pRPA2-High if > 16% cells had ≥ 2 foci. Results In the discovery cohort, HR-proficient, demonstrated significantly higher rates pathologic complete response than pRPA2-Low tumors. longer survival after those Additionally, assay effectively predicted outcomes recurrent samples. Conclusion Our study underscores importance considering markers alongside HR status therapeutic planning. work suggest that this could used throughout patient’s course expand number receiving effective while reducing unnecessary toxicity.

Язык: Английский

Positioning Loss of PARP1 Activity as the Central Toxic Event in BRCA-Deficient Cancer DOI Creative Commons
Nathan J. MacGilvary, Sharon B. Cantor

DNA repair, Год журнала: 2024, Номер 144, С. 103775 - 103775

Опубликована: Окт. 19, 2024

The mechanisms by which poly(ADP-ribose) polymerase 1 (PARP1) inhibitors (PARPi)s inflict replication stress and/or DNA damage are potentially numerous. PARPi toxicity could derive from loss of its catalytic activity physical trapping PARP1 onto that perturbs not only function in repair and replication, but also obstructs compensating pathways. combined disruption with either the hereditary breast ovarian cancer genes, BRCA1 or BRCA2 (BRCA), results synthetic lethality. This has driven development PARP as therapies for BRCA-mutant cancers. In this review, we focus on recent findings highlight activity, rather than PARPi-induced allosteric trapping, central to efficacy BRCA deficient cells. However, review PARP-trapping is an effective strategy other genetic deficiencies. Together, conclude mechanism-of-action unilateral; enhanced differentially killing depending context. Therefore, effectively targeting cells requires intricate understanding their key underlying vulnerabilities.

Язык: Английский

Процитировано

4

Acid-Responsive Biocompatible Hydrogel Modulating Tumor DNA Self-Repair Collaborated with Chemotherapy for Boosting STING Pathway-Associated Immunotherapy DOI

Yan-Tong Lin,

Ran Meng,

Shi‐Man Zhang

и другие.

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

In this study, an acid-responsive hydrogel (ODCM@AZD) encapsulating doxorubicin (DOX), Mn2+, and AZD2281 is rationally engineered for synergistic chemo-immunotherapy. Notably, ODCM@AZD can be specifically degraded within the tumor microenvironment to release loaded therapeutic agents. Specifically, released DOX kills cells produce abundant cytoplasmic DNA, while freed inhibits DNA repair pathway of enhance chemotherapeutic effect promote accumulation damaged which further aggravated by reactive oxygen species (ROS) generated from Mn2+-mediated Fenton-like reaction. Not only that, Mn2+ simultaneously increases sensitivity cGAS stimulate cGAS-STING synergizes with DOX-mediated immunogenic cell death (ICD) initiate powerful antitumor immune responses. More importantly, combination checkpoint blockade (ICB) significantly improves systemic tumoricidal immunity potentiates effects on distant recurrent models, providing a new idea

Язык: Английский

Процитировано

0

Role of PARP Inhibitors: A New Hope for Breast Cancer Therapy DOI Open Access

K De,

Malabendu Jana,

Bhabadeb Chowdhury

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2773 - 2773

Опубликована: Март 19, 2025

Tumors formed by the unchecked growth of breast cells are known as cancer. The second most frequent cancer in world is It common among females. In 2022, 2,296,840 women were diagnosed with therapy evolving through development Poly (ADP-ribose) polymerase (PARP) inhibitors, which offering people specific genetic profiles new hope research into disease continues. focuses on patients BRCA1 and BRCA2 mutations. This review summarizes recent mechanisms action PARP inhibitors their implications for therapy. We how therapeutic applications developing highlight studies showing effectiveness these medicines whether used alone or combination. Furthermore, significance customized highlighted enhancing patient outcomes we address function testing identifying candidates inhibition. Recommendations future areas to maximize potential also included, along challenges limits clinical usage. objective this improve our comprehension complex interaction between biology knowledge will help guide screening approaches, practice, support preventive initiatives at risk.

Язык: Английский

Процитировано

0

HDAC-driven mechanisms in anticancer resistance: epigenetics and beyond DOI Open Access

Martina Minisini,

Massimo Mascaro,

Claudio Brancolini

и другие.

Cancer Drug Resistance, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 20, 2024

The emergence of drug resistance leading to cancer recurrence is one the challenges in treatment patients. Several mechanisms can lead resistance, including epigenetic changes. Histone deacetylases (HDACs) play a key role chromatin regulation through and are also involved resistance. control histone acetylation accessibility regulatory DNA sequences such as promoters, enhancers, super-enhancers known by which HDACs influence gene expression. Other targets that not histones contribute This review describes contribution that, some cases, may determine chemotherapy or other treatments.

Язык: Английский

Процитировано

3

Ginseng extract (Ginsenoside RG3) combined with STING agonist reverses TAM/M2 polarization to inhibit TNBC evolution DOI Creative Commons
Qiang Fu, Zhongqi Lu, Ying Chang

и другие.

Industrial Crops and Products, Год журнала: 2024, Номер 222, С. 119589 - 119589

Опубликована: Сен. 9, 2024

Язык: Английский

Процитировано

1

Targeting DNA damage response in pancreatic ductal adenocarcinoma: A review of preclinical and clinical evidence DOI
Fatemeh Moosavi, Bahareh Hassani, Somayeh Nazari

и другие.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2024, Номер unknown, С. 189185 - 189185

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

1

Synergy trap for guardian angels of DNA: Unraveling the anticancer potential of phthalazinone-thiosemicarbazone hybrids through dual PARP-1 and TOPO-I inhibition DOI

Eman M Elkafoury,

Mervat H. El‐Hamamsy, Eman A. El-Bastawissy

и другие.

Bioorganic Chemistry, Год журнала: 2024, Номер 153, С. 107924 - 107924

Опубликована: Окт. 30, 2024

Язык: Английский

Процитировано

1

Advancements in Clinical Research and Emerging Therapies for Triple-Negative Breast Cancer Treatment DOI
Lili Xu, Pengtao Xu, Jingsong Wang

и другие.

European Journal of Pharmacology, Год журнала: 2024, Номер unknown, С. 177202 - 177202

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

1

Application and research progress of synthetic lethality in the development of anticancer therapeutic drugs DOI Creative Commons
Xiaoliang Gong, Chunxi Liu, Haoyang Tang

и другие.

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Ноя. 25, 2024

With the tremendous success of PARP inhibitor olaparib in clinical practice, synthetic lethality has become an important field for discovery and development anticancer drugs. More more targets have been discovered with rapid biotechnology recent years. Currently, many drug candidates that were designed developed on basis concept entered trials. Taking representative lethal Poly ADP-ribose polymerase 1 (PARP1), Werner syndrome helicase (WRN) protein arginine methyltransferase 5 (PRMT5) as examples, this article briefly discusses application research progress

Язык: Английский

Процитировано

0

Replication stress marker phospho-RPA2 predicts response to platinum and PARP inhibitors in homologous recombination-proficient ovarian cancer DOI Open Access
Angela M. Schab,

Amanda Compadre,

Richard Drexler

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

ABSTRACT Background Ovarian cancer treatment includes cytoreductive surgery, platinum-based chemotherapy, and often poly (ADP-ribose) polymerase (PARP) inhibitors. Homologous recombination (HR)-deficiency is a well-established predictor of therapy sensitivity. However, over 50% HR-proficient tumors also exhibit sensitivity to standard-of-care treatments. Currently, there are no biomarkers identify which will be sensitive therapy. Replication stress may serve as key determinant response. Methods We evaluated phospho-RPA2-T21 (pRPA2) foci via immunofluorescence potential biomarker replication in formalin-fixed, paraffin-embedded tumor samples collected at diagnosis from patients treated with platinum chemotherapy (discovery cohort: n = 31, validation 244) or PARP inhibitors (n 87). Recurrent 37) were analyzed. pRPA2 scores calculated using automated imaging analysis. Samples defined pRPA2-High if > 16% cells had ≥ 2 foci. Results In the discovery cohort, HR-proficient, demonstrated significantly higher rates pathologic complete response than pRPA2-Low tumors. longer survival after those Additionally, assay effectively predicted outcomes recurrent samples. Conclusion Our study underscores importance considering markers alongside HR status therapeutic planning. work suggest that this could used throughout patient’s course expand number receiving effective while reducing unnecessary toxicity.

Язык: Английский

Процитировано

0