Prevalence and diversity of spotted fever group Rickettsia species in ixodid ticks from domestic dogs in Chad, Africa DOI Creative Commons

Stephanie Osip,

Morgan Friedman,

Ellen Haynes

и другие.

Ticks and Tick-borne Diseases, Год журнала: 2024, Номер 15(6), С. 102405 - 102405

Опубликована: Окт. 19, 2024

Tick-borne pathogens in the genus Rickettsia are causative agents of severe and potentially fatal spotted fever group (SFG) typhus diseases dogs humans. Climate, habitat, land-use changes impacting vector ranges, with expansions resulting novel being introduced into naïve locations. Despite public health importance SFG Rickettsia, there relatively few data on prevalence diversity rickettsial sub-Saharan Africa. The aim this study was to characterize ixodid ticks (104 Amblyomma spp., 160 Rhipicephalus one Hyalomma truncatum) collected from domestic Chad, Ticks were screened for spp. using a nested PCR targeting 17-kDa gene. Species identification through bidirectional Sanger sequencing 17-kDa, ompA, ompB, and/or gltA gene targets. A total 43.3 % (115/265) positive six species identified: R. africae, massiliae, conorii, felis, monacensis Candidatus muridii. Seven additional samples undetermined species. an important zoonotic pathogen, found 81 (79/97) A. variegatum 29 (2/7) marmoreum complex species, that infests wide range birds mammals, including Finally, we detected high most which zoonotic, Rh. muhsamae. Collectively these indicate is risk rickettsiosis Chad further studies region warranted.

Язык: Английский

New insights into the molecular phylogeny, biogeographical history, and diversification of Amblyomma ticks (Acari: Ixodidae) based on mitogenomes and nuclear sequences DOI Creative Commons
Juan E. Uribe, Samuel Kelava, Santiago Nava

и другие.

Parasites & Vectors, Год журнала: 2024, Номер 17(1)

Опубликована: Март 18, 2024

Abstract Background Amblyomma is the third most diversified genus of Ixodidae that distributed across Indomalayan, Afrotropical, Australasian (IAA), Nearctic and Neotropical biogeographic ecoregions, reaching in Neotropic its highest diversity. There have been hints previously published phylogenetic trees from mitochondrial genome, nuclear rRNA, combinations both morphology or plus species southern cone South America, might be sister-group to rest world. However, a stable framework for better understanding patterns underpinning diversification lacking. Methods We used genomic techniques sequence complete nearly genomes –ca. 15 kbp– as well ribosomal cluster 8 17 ticks order study phylogeny pattern , with particular emphasis on region. The new information generated here together available 43 (22 other 21 hard ticks–as outgroup–) were perform probabilistic methods inferences time-tree estimation using dates. Results In present paper, we strongest evidence yet may indeed world (species occur mainly Afrotropical zoogeographic regions). Our results showed all subgenera ( Cernyomma Anastosiella Xiphiastor Adenopleura Aponomma Dermiomma ) are not monophyletic, except Walkeriana . Likewise, our best scenario supports origin posterior hemisphere at 47.8 36.8 Mya, respectively. This could associated end connection Australasia ecoregions by Antarctic land bridge. Also, analyses let us see colonization some neotropical Nearctic. Conclusions found strong main theater was hemisphere, potentially driven Bridge's intermittent late Eocene. addition, subgeneric classification lacks evolutionary support. Future studies denser taxonomic sampling lead findings relationships history genus. Graphical

Язык: Английский

Процитировано

7

Intra- and interspecific variation of Amblyomma ticks from southern Africa DOI Creative Commons
Andeliza Smit, Fernando C. Mulandane,

Martinet Labuschagne

и другие.

Parasites & Vectors, Год журнала: 2024, Номер 17(1)

Опубликована: Авг. 28, 2024

Amblyomma spp. ticks, known for their long mouthparts, bright ornate appearance and aggressive hunting behaviour, are vectors of a number important pathogens. In southern Africa, 17 currently documented. Of these species, hebraeum variegatum have been well studied due to wide geographical range status as competent pathogens that veterinary medical importance. Studies on other in Africa neglected, fostering ongoing debates the validity certain species such pomposum. This study investigated inter- intra-species variation ticks collected focusing resolving dispute about A. pomposum being distinct species.

Язык: Английский

Процитировано

3

Molecular Detection and Phylogenetic Characterization of Rickettsia in Ticks Collected from Leopard Tortoise (Geochelone pardalis) in rural Zambia DOI Creative Commons
Cynthia Sipho Khumalo,

Malala Mulavu,

Katendi Changula

и другие.

Science in One Health, Год журнала: 2024, Номер 3, С. 100074 - 100074

Опубликована: Янв. 1, 2024

In sub-Saharan Africa, limited studies have investigated zoonotic pathogens that may be harboured by ticks infesting reptiles such as tortoises. Here, we report the presence of pathogenic Rickettsia in (Amblyomma marmoreum) collected from leopard tortoise (Geochelone pardalis) rural Zambia. Using polymerase chain reaction, 56% (49/87) were positive for outer membrane protein (ompB) gene. Multi-locus sequence and phylogenetic analysis based on ompB, ompA, citrate synthase (gltA) genes showed carried africae, other spp. closely related to R. raoultii, massiliae, tamurae monacensis. Given proximity between humans, livestock, wildlife these habitats, there exists a considerable risk transmission human populations this setting. These results call heightened awareness further research into dynamics tick-borne diseases regions where humans animals coexist, particularly context tortoise-associated vectors. Understanding addressing potential disease vectors is crucial effective public health measures prevention zoonoses.

Язык: Английский

Процитировано

1

Amblyomma sparsum Neumann 1899 on migratory birds from Africa: First records in Italy DOI Creative Commons
Michela Menegon, Elisa Mancuso, Marco Di Luca

и другие.

Ticks and Tick-borne Diseases, Год журнала: 2024, Номер 15(6), С. 102387 - 102387

Опубликована: Авг. 7, 2024

Migratory birds play an important role in transporting ixodid ticks and tick-borne pathogens between continents. During the Boreal spring, migratory reach Europe, mainly from sub-Saharan Africa or northern African countries but not much is known about diversity ecology of they spread. From 2017 to 2022, framework two consecutive projects focused on sampling a total 27 immature Amblyomma were collected birds, belonging 8 species, captured Island Ventotene, stop-over site Mediterranean Sea. In absence adult specimens, morphological identification was limited assigning these genus. this study, sequencing comparative analysis three mitochondrial molecular markers (12S rDNA, 16S COI) performed achieve taxonomic identification. Sequences obtained Ventotene specimens matched at 100% identity with sparsum. conclusion, study documented that stages species marmoreum complex reached Pontine Islands for six years. The entry alien tick their potentially transmitted deserves further also light globally ongoing climate change.

Язык: Английский

Процитировано

0

Prevalence and diversity of spotted fever group Rickettsia species in ixodid ticks from domestic dogs in Chad, Africa DOI Creative Commons

Stephanie Osip,

Morgan Friedman,

Ellen Haynes

и другие.

Ticks and Tick-borne Diseases, Год журнала: 2024, Номер 15(6), С. 102405 - 102405

Опубликована: Окт. 19, 2024

Tick-borne pathogens in the genus Rickettsia are causative agents of severe and potentially fatal spotted fever group (SFG) typhus diseases dogs humans. Climate, habitat, land-use changes impacting vector ranges, with expansions resulting novel being introduced into naïve locations. Despite public health importance SFG Rickettsia, there relatively few data on prevalence diversity rickettsial sub-Saharan Africa. The aim this study was to characterize ixodid ticks (104 Amblyomma spp., 160 Rhipicephalus one Hyalomma truncatum) collected from domestic Chad, Ticks were screened for spp. using a nested PCR targeting 17-kDa gene. Species identification through bidirectional Sanger sequencing 17-kDa, ompA, ompB, and/or gltA gene targets. A total 43.3 % (115/265) positive six species identified: R. africae, massiliae, conorii, felis, monacensis Candidatus muridii. Seven additional samples undetermined species. an important zoonotic pathogen, found 81 (79/97) A. variegatum 29 (2/7) marmoreum complex species, that infests wide range birds mammals, including Finally, we detected high most which zoonotic, Rh. muhsamae. Collectively these indicate is risk rickettsiosis Chad further studies region warranted.

Язык: Английский

Процитировано

0