Fourteen anti-tick vaccine targets are variably conserved in cattle fever ticks
Parasites & Vectors,
Год журнала:
2025,
Номер
18(1)
Опубликована: Апрель 15, 2025
Abstract
Background
Rhipicephalus
(
Boophilus
)
microplus
causes
significant
cattle
production
losses
worldwide
because
it
transmits
Babesia
bovis
and
B.
bigemina
,
the
causative
agents
of
bovine
babesiosis.
Control
these
ticks
has
primarily
relied
on
treatment
with
chemical
acaricides,
but
frequent
use,
exacerbated
by
one-host
lifecycle
ticks,
led
to
high-level
resistance
multiple
classes
acaricides.
Consequently,
new
approaches
for
control,
such
as
anti-tick
vaccines,
are
critically
important.
Key
this
approach
is
targeting
highly
conserved
antigenic
epitopes
reduce
risk
vaccine
escape
in
heterologous
tick
populations.
Methods
We
evaluated
amino
acid
conservation
within
14
proteins
across
167
R.
collected
from
geographically
diverse
locations
Americas
Pakistan
using
polymerase
chain
reaction
(PCR)
amplicon
sequencing
silico
translation
exons.
Results
found
that
varied
considerably
proteins.
Only
voltage-dependent
anion
channel
(VDAC)
was
fully
all
samples
(protein
similarity
1.0).
Four
other
were
conserved:
aquaporin
RmAQP1
(0.989),
vitellogenin
receptor
(0.985),
serpin-1
subolesin
(0.981).
In
contrast,
glycoprotein
Bm86
one
least
(0.889).
The
sequence
used
original
Australian
TickGARD
carried
many
replacements
compared
populations
examined
here,
supporting
hypothesis
target
not
optimal
use
Americas.
By
mapping
onto
predicted
three-dimensional
(3D)
protein
models,
we
also
identified
changes
several
small-peptide
vaccines
portions
RmAQP2,
chitinase,
Bm86.
Conclusions
These
findings
emphasize
importance
thoroughly
analyzing
variation
targets
before
selecting
candidate
antigens.
When
considering
alone,
RmAQP1,
receptor,
serpin-1,
subolesin,
especially
VDAC
rank
high-priority
candidates
perhaps
globally.
Язык: Английский
The Rhipicephalus sanguineus group: updated list of species, geographical distribution, and vector competence
Parasites & Vectors,
Год журнала:
2024,
Номер
17(1)
Опубликована: Дек. 27, 2024
Abstract
The
Rhipicephalus
sanguineus
group
is
an
assembly
of
species
morphologically
and
phylogenetically
related
to
sensu
stricto.
taxonomy
systematics
this
have
remained
obscure
for
a
long
time,
but
extensive
research
conducted
during
the
past
two
decades
has
closed
many
knowledge
gaps.
These
advancements
culminated
in
redescription
R.
stricto,
with
subsequent
revalidation
former
synonyms
(
linnaei
,
rutilus
secundus
)
even
description
new
afranicus
hibericus
).
With
much
clearer
picture
these
species,
we
present
updated
list
belonging
group,
along
review
their
geographic
distribution
vector
role
various
pathogens
animals
humans.
We
also
identify
gaps
be
bridged
future
studies.
Graphical
abstract
Язык: Английский
Mutations in the voltage-gated sodium channel associated with permethrin resistance in Rhipicephalus linnaei populations in Thailand
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 2, 2025
Abstract
Insecticide
resistance
is
a
serious
threat
to
vector
control
programs
worldwide.
Therefore,
it
crucial
monitor
the
development
of
in
populations.
Rhipicephalus
linnaei
(Audouin,
1826)
cosmopolitan
tick
and
medically
important
pathogens.
We
conducted
comprehensive
investigation
permethrin
larvae
Rh.
populations
across
Thailand
by
comparing
phenotypic
with
genotypes,
focusing
on
mutations
Domain
II
III
voltage-gated
sodium
channel
gene.
Results
showed
that
obtained
from
engorged
female
have
developed
permethrin,
levels
varying
location.
Resistance
ratios
ranged
1
56
when
compared
least
susceptible
local
population.
Genotyping
identified
at
positions
190
(c.190C
>
A
c.190C
G)
2134
(c.2134T
C)
III,
respectively,
which
are
correlated
resistance.
new
alleles
c.190CG
c.190AG
highly
resistant
Phasichareon,
Bangkok,
Chonburi
provinces.
This
study
provides
first
evidence,
our
knowledge,
ticks
Thailand.
Elevated
indicate
veterinarians
farmers
should
consider
products
alternative
modes
action.
Язык: Английский
Leading anti-tick vaccine targets are variably conserved in cattle fever ticks
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 28, 2024
Abstract
Background
Rhipicephalus
(
Boophilus)
microplus
causes
significant
cattle
production
losses
worldwide
because
it
transmits
Babesia
bovis
and
B.
bigemina
causative
agents
of
bovine
babesiosis.
Control
these
ticks
primarily
has
relied
on
treatment
with
chemical
acaricides,
but
frequent
use,
exacerbated
by
the
one-host
life
cycle
ticks,
led
to
high-level
resistance
multiple
classes
acaricides.
Consequently,
new
approaches
for
control,
such
as
anti-tick
vaccines,
are
critically
important.
Key
this
approach
is
targeting
highly
conserved
antigenic
epitopes
reduce
risk
vaccine
escape
in
heterologous
tick
populations.
Methods
We
evaluated
amino
acid
conservation
within
14
proteins
across
167
R.
microplus
collected
from
geographically
diverse
locations
Americas
Pakistan
using
PCR
amplicon
sequencing
in
silico
translation
exons.
Results
found
that
varied
considerably
proteins.
Only
one
target,
voltage-dependent
anion
channel,
was
fully
all
samples
(protein
similarity
1.0).
Five
other
were
conserved:
aquaporin
RmAQP1
(0.989),
vitellogenin
receptor
(0.985),
serpin-1
subolesin
(0.981).
In
contrast,
glycoprotein
protease
Bm86
least
(0.889).
The
sequence
used
original
Australian
TickGARD
carried
many
replacements
compared
populations
examined
here,
supporting
hypothesis
target
not
optimal
use
Americas.
By
mapping
onto
predicted
3D
protein
models,
we
also
identified
changes
several
small
peptide
vaccines
portions
RmAQP2,
chitinase,
Bm86.
Conclusions
These
findings
emphasize
importance
thoroughly
analyzing
variation
targets
before
selecting
candidate
antigens.
When
considering
alone,
RmAQP1,
receptor,
serpin-1,
subolesin,
especially
channel
rank
high
priority
candidates
perhaps
globally.
Язык: Английский
Molecular Detection of Kinetoplastid Species in Ticks and Fleas Associated with Dogs and Humans in Mexico
Pathogens,
Год журнала:
2024,
Номер
13(12), С. 1072 - 1072
Опубликована: Дек. 6, 2024
The
Trypanosomatidae
family
encompasses
around
24
genera
of
unicellular
protozoans,
many
which
are
transmitted
by
various
hematophagous
arthropods,
particularly
members
the
Orders
Diptera
and
Hemiptera.
Fleas
ticks—an
understudied
group
ectoparasites—have
been
shown
to
be
hosts
a
wide
crescent
variety
trypanosomatid
species.
Further,
fleas
ticks
companion
animals
have
neglected
in
surveillance
despite
proximity
human
populations
anthropophagous
habits
these
can
potentially
act
as
vectors
zoonotic
trypanosomatids.
We
aimed
identify
presence,
characterize
species,
establish
prevalence
Kinetoplastids,
including
family,
ectoparasites
collected
from
dogs
humans
Mexico.
A
total
537
ectoparasite
specimens
belonging
six
taxa
(Amblyomma
mixtum,
A.
tenellum,
Ctenocephalides
felis
felis,
Pulex
simulans,
Rhipicephalus
linnaei,
Rh.
sanguineus
s.s.)
were
15
States
An
800
bp
fragment
18S-rDNA
gene
kinetoplastids
was
amplified
sequenced.
presence
two
agents
(Trypanosoma
caninum
Parabodo
sp.)
detected
R.
linnaei
one
(Blechomonas
lauriereadi)
cat
flea
Ct.
felis.
This
is
first
record
genetic
material
kinetoplastid
species
Язык: Английский