Methyltransferase 3-mediated m6A modification of Switch/sucrose non-fermenting-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 promotes mycobacterium tuberculosis-infected macrophage M1 polarization and inflammation

CytoJournal, Год журнала: 2025, Номер 22, С. 38 - 38

Опубликована: Апрель 1, 2025

Objective Mycobacterium tuberculosis (MTB) manipulates macrophage functions, thus mediating (TB) progression. Whether the switch/sucrose non-fermenting-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 (SMARCA5) mediates MTB-induced polarization remains unclear. Material and Methods Human Promyelocytic Leukemia Cell Line was induced into macrophages then treated with MTB. viability apoptosis were tested cell counting kit 8 assay flow cytometry. Classically activated (M1) inflammation measured by detecting CD86 + rate inflammatory factor levels. The levels SMARCA5, methyltransferase 3 (METTL3), insulin-like growth 2 binding protein 1 (IGF2BP1) assessed using quantitative real-time polymerase chain reaction or Western blot. interaction between SMARCA5 METTL3 IGF2BP1 confirmed methylated RNA immunoprecipitation (RIP) RIP assays. effect knockdown on messenger (mRNA) stability evaluated actinomycin D treatment. Results MTB treatment suppressed promoted M1 ( P < 0.05), abolished these effects 0.05). mediated m6A methylation to enhance mRNA latter, this modification recognized IGF2BP1. upregulation reverted si-METTL3-mediated inhibition Conclusion METTL3-mediated facilitates inflammation, providing novel target for TB

Язык: Английский

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Electroacupuncture Serum Alleviates Ogd/R-Induced Astrocyte Damage by Regulating the AQP4 Via m6A Methylation of lncRNA MALAT1

Neurochemical Research, Год журнала: 2025, Номер 50(2)

Опубликована: Апрель 1, 2025

Язык: Английский

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Role and regulators of N6-methyladenosine (m6A) RNA methylation in inflammatory subtypes of asthma: a comprehensive review

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июль 23, 2024

Asthma is a common chronic inflammatory disease of the lungs and airway, yet its subtypes potential pathogenesis have not been completely elucidated require further study. With advances in epigenetic development, methylation has emerged as new direction for identifying decoding occurrence subtype manifestations asthma. N 6 -methyladenosine (m A), an RNA modification occurring -position adenosine, prevalent observed eukaryotes. It exerts significant control over mRNA metabolism by regulating alternative splicing, stability, export, translation. The dynamic process m A plays crucial role asthma tightly regulated three types regulators: writers, readers, erasers. This article provides comprehensive review association between regulators asthma, such involvement cells related response. Furthermore, findings presented herein provide insights solid foundation research on biomarkers diagnosis development personalized treatment different particularly neutrophilic eosinophilic

Язык: Английский

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Participation of TLRs in cancer immunopathogenesis and drug resistance via interacting with immunological and/or non-immunological signaling pathways as well as lncRNAs

International Immunopharmacology, Год журнала: 2024, Номер 140, С. 112764 - 112764

Опубликована: Июль 29, 2024

Язык: Английский

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Host Long Noncoding RNAs as Key Players in Mycobacteria–Host Interactions

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2656 - 2656

Опубликована: Дек. 21, 2024

Mycobacterial infections, caused by various species within the Mycobacterium genus, remain one of main challenges to global health across world. Understanding complex interplay between host and mycobacterial pathogens is essential for developing effective diagnostic therapeutic strategies. Host long noncoding RNAs (lncRNAs) have emerged as key regulators in cellular response bacterial infections cells. This review provides an overview intricate relationship lncRNAs context tuberculosis non-tuberculous mycobacterium (NTM) infections. Accumulation evidence indicates that play a critical role regulating infection cells, such macrophages, primary cells intracellular survival. The expression specific has been implicated pathogenesis providing potential targets development novel host-directed therapies biomarkers TB diagnosis. In summary, this aims highlight current state knowledge regarding involvement It also emphasizes their application targets.

Язык: Английский

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Non-coding RNAs as a Critical Player in the Regulation of Inflammasome in Inflammatory Bowel Diseases; Emphasize on lncRNAs

Cell Biochemistry and Biophysics, Год журнала: 2024, Номер unknown

Опубликована: Окт. 19, 2024

Язык: Английский

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