Ultrasonic Modulation of Astrocytic and Neuronal Calcium Dynamics in Mouse Cortex DOI Open Access
Yu Yong, Hao Jiang,

C. F. Qiao

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Abstract Background Astrocytes are abundant in the brain and their calcium signaling is reported to have an important effect on neuronal activity both physiological pathological conditions. Low-frequency focused ultrasound (FUS) has recently emerged as a powerful noninvasive neuromodulation approach, yet its impact astrocyte dynamics different states vivo poorly understood. Objective This study aimed elucidate effects of non-thermal FUS dynamic with cellular-resolution cell-type-specific recording identify whether influences cortical astrocytes neurons distinctive state dependent. Methods Here we combined customized 0.521MHz transducer two-photon microscopy, allowing simultaneous single-cell resoultion imaging stimulation at intensities 0.91 or 1.5 W/cm 2 examine responses somatosensory cortex awake lightly anesthetized mice. Functional clustering analysis was performed response activated inhibited subpopulations. Results In mice, significantly enhanced amplitude, frequency, temporal integral transients, while suppressing reducing proportion contrast, mice displayed blunted increased negligible modulation under FUS, suggesting that baseline suppression from anesthesia partially masks effects. Conclusions Our demonstrated elicited distinctive, state-dependent neurons, highlighting previously underappreciated targets neuromodulation. These findings will pave way for FUS-based therapies targeting astrocyte–neuron interactions conditions involving abnormal excitability. Highlights Low duty cycle low frequency induced heat Ultrasound markedly transients suppressed The cell types were reduced light anesthesia.

Язык: Английский

Ultrasonic Modulation of Astrocytic and Neuronal Calcium Dynamics in Mouse Cortex DOI Open Access
Yu Yong, Hao Jiang,

C. F. Qiao

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Abstract Background Astrocytes are abundant in the brain and their calcium signaling is reported to have an important effect on neuronal activity both physiological pathological conditions. Low-frequency focused ultrasound (FUS) has recently emerged as a powerful noninvasive neuromodulation approach, yet its impact astrocyte dynamics different states vivo poorly understood. Objective This study aimed elucidate effects of non-thermal FUS dynamic with cellular-resolution cell-type-specific recording identify whether influences cortical astrocytes neurons distinctive state dependent. Methods Here we combined customized 0.521MHz transducer two-photon microscopy, allowing simultaneous single-cell resoultion imaging stimulation at intensities 0.91 or 1.5 W/cm 2 examine responses somatosensory cortex awake lightly anesthetized mice. Functional clustering analysis was performed response activated inhibited subpopulations. Results In mice, significantly enhanced amplitude, frequency, temporal integral transients, while suppressing reducing proportion contrast, mice displayed blunted increased negligible modulation under FUS, suggesting that baseline suppression from anesthesia partially masks effects. Conclusions Our demonstrated elicited distinctive, state-dependent neurons, highlighting previously underappreciated targets neuromodulation. These findings will pave way for FUS-based therapies targeting astrocyte–neuron interactions conditions involving abnormal excitability. Highlights Low duty cycle low frequency induced heat Ultrasound markedly transients suppressed The cell types were reduced light anesthesia.

Язык: Английский

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