Amino acid substitutions in NSP6 and NSP13 of SARS-CoV-2 contribute to superior virus growth at low temperatures
Journal of Virology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
ABSTRACT
In
general,
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
replicates
well
at
37°C,
which
is
the
temperature
of
human
lower
tract,
but
it
poorly
30°C‒32°C,
upper
tract.
The
replication
efficiency
SARS-CoV-2
in
tract
may
directly
affect
its
transmissibility.
this
study,
an
XBB.1.5
isolate
showed
superior
replicative
ability
32°C
and
30°C,
whereas
most
other
Omicron
sub-variant
isolates
limited
growth.
Deep
sequencing
analysis
demonstrated
that
frequencies
viruses
possessing
NSP6-S163P
NSP13-P238S
substitutions
increased
to
more
than
97%
during
propagation
did
not
reach
55%
37°C.
Reverse
genetics
revealed
these
contributed
virus
growth
vitro
low
temperatures
by
improving
genome
replication.
Mutant
both
slightly
higher
titers
hamsters
compared
parental
virus;
however,
transmissibility
between
was
similar
for
mutant
viruses.
Taken
together,
our
findings
indicate
contribute
hamsters.
IMPORTANCE
Severe
efficiently
However,
airway
30°C–32°C.
Therefore,
could
influence
we
assessed
sub-variants
found
ability.
By
deep
reverse
genetics,
amino
acid
changes
NSP6
NSP13
low-temperature
growth;
improved
RNA
polymerase
activity
enhanced
Although
alone
drastically
transmissibility,
combination
with
substitutions,
they
humans.
Furthermore,
since
enhance
cultured
cells,
be
used
improve
production
inactivated
or
live
attenuated
vaccine
virus.
Язык: Английский
SARS-CoV-2 virus lacking the envelope and membrane open-reading frames as a vaccine platform
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Май 13, 2025
Язык: Английский
Live attenuated COVID-19 vaccines: approaches to development and prospects for clinical use
Journal of microbiology epidemiology immunobiology,
Год журнала:
2023,
Номер
100(3), С. 225 - 236
Опубликована: Июль 11, 2023
Although
WHO
declared
an
end
to
the
pandemic,
COVID-19
remains
a
significant
public
health
concern
worldwide.
Modern
vaccines
often
induce
either
only
humoral
or
cellular
immunity.
Furthermore,
new
emergent
epidemiologically
SARS-CoV-2
variants
and
their
spread
considerably
reduce
effectiveness
of
preventive
vaccination.
Therefore,
there
is
urgent
need
improve
existing
against
COVID-19.
One
promising
approaches
solution
problem
creation
"universal"
vaccine
that
would
have
cross-protective
activity
different
antigenic
virus.
In
this
respect,
development
live
attenuated
special
interest,
as
it
can
activate
not
humoral,
but
also
cell-mediated
components
immunity,
providing
long-term
immune
response
cross-protection
virus.
This
review
highlights
producing
strains
gives
assessment
prospects
for
clinical
use.
Some
researchers
use
methods
genetic
engineering
reverse
genetics
such
site-directed
mutagenesis
codon
deoptimization
virus
attenuation.
Others
tend
traditional
focusing
on
mutants
through
extended
passaging
in
cell
culture
under
selective
conditions.
The
gained
experience
demonstrates
great
highly
effective
live-attenuated
Язык: Английский
Single intranasal immunization with attenuated Wuhan-like SARS-CoV-2 provides highly effective cross-protection against Delta and Omicron VOCs
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 9, 2023
Abstract
Despite
the
end
of
COVID-19
pandemic,
problem
vaccine
prevention
this
disease
appears
highly
relevant.
The
emergence
and
widespread
distribution
Omicron
SARS-CoV-2
variant
concern
(VOC)
its
sublineages
has
dramatically
reduced
efficacy
vaccination
with
licensed
vaccines.
possible
approach
to
solving
is
develop
a
nasal
live
attenuated
(LAV)
capable
activating
humoral,
mucosal,
cell-mediated
immunity,
providing
prolonged
immune
response
cross-protection
against
different
VOCs.
aim
present
study
was
determine
immunization
cold-adapted
Wuhan-like
D-D2
strain
homologous
heterologous
challenges.
Single
intranasal
golden
Syrian
hamsters
showed
high
immunogenicity:
seroconversion
evident
in
all
immunized
animals.
absence
infectious
virus
lung,
passages,
brains
animals
allows
us
characterize
immunity
as
"sterile"
consistent
viral
RNA
concentration
organs
on
fourth-day
post-challenge.
Comparison
data
reproduction
organs,
weight
dynamics,
morphological
changes
lungs
unimmunized
that
provides
effective
protection
infection
development
pneumonia
when
challenged
both
ancestral
Delta
(B.1.617.2.122)
(sublineages
BA.1
BA.5.2)
variants.
Thus,
variants
have
great
potential
for
vaccines
their
basis.
Язык: Английский
Single intranasal immunization with attenuated Wuhan-like SARS-CoV-2 provides highly effective cross-protection against Delta and Omicron variants of concern
Journal of microbiology epidemiology immunobiology,
Год журнала:
2024,
Номер
101(1), С. 36 - 51
Опубликована: Март 9, 2024
Introduction.
Despite
the
end
of
COVID-19
pandemic,
problem
vaccine
prevention
this
disease
appears
highly
relevant.
The
emergence
and
widespread
distribution
Omicron
SARS-CoV-2
variant
concern
(VOC)
its
sublineages
has
dramatically
reduced
efficacy
vaccination.
possible
approach
to
solving
is
develop
a
nasal
live
attenuated
capable
activating
humoral,
mucosal,
cell-mediated
immunity,
providing
prolonged
immune
response
cross-protection
against
different
VOCs.
aim
study
was
determine
immunization
with
cold-adapted
Wuhan-like
D-D2
strain
homologous
heterologous
challenges.
Materials
methods.
conducted
on
an
animal
model
coronavirus
pneumonia
in
golden
Syrian
hamsters.
assessed
by
comparing
dynamics
weight,
viral
load
organs
histopathological
changes
lungs
immunized
unimmunized
animals.
Results.
Single
intranasal
hamsters
showed
high
immunogenicity:
seroconversion
evident
all
provides
effective
protection
development
productive
infection
when
challenged
both
ancestral
virus
strains
related
Delta
(AY.122)
(sublineages
BA.1.1
BA.5.2)
variants.
Conclusion.
attenuation
promising
strategy
for
vaccine.
Язык: Английский