Research Integrity and Peer Review,
Год журнала:
2024,
Номер
9(1)
Опубликована: Окт. 6, 2024
Abstract
Background
Preprints
are
scientific
articles
that
have
not
undergone
the
peer-review
process.
They
allow
latest
evidence
to
be
rapidly
shared,
however
it
is
unclear
whether
they
can
confidently
used
for
decision-making
during
a
public
health
emergency.
This
study
aimed
compare
data
and
quality
of
preprints
released
first
four
months
2022
mpox
outbreak
their
published
versions.
Methods
Eligible
(
n
=
76)
posted
between
May
August
were
identified
through
an
established
literature
database
followed
July
2024
changes
in
publication
status.
Quality
studies
was
assessed
by
two
independent
reviewers
evaluate
quality,
using
validated
tools
available
design
33).
Tools
included
Newcastle-Ottawa
Scale;
Assessment
Diagnostic
Accuracy
Studies
2
(QUADAS-2);
JBI
Critical
Appraisal
Checklists.
The
questions
each
tool
led
overall
assessment
high
(no
concerns
with
design,
conduct,
and/or
analysis),
moderate
(minor
concerns)
or
low
(several
concerns).
Changes
(e.g.
methods,
outcomes,
results)
preprint-published
pairs
60)
one
reviewer
verified
second.
Results
versions
could
evaluated
25
pairs)
mostly
as
quality.
Minimal
no
change
from
preprint
identified:
all
observational
(10/10),
most
case
series
(6/7)
surveillance
analyses
(3/3)
had
while
some
diagnostic
test
accuracy
(3/5)
improved
worsened
scores.
Among
60),
outcomes
often
added
version
(58%)
less
commonly
removed
(18%).
Numerical
results
changed
53%
studies,
these
(22/32)
minor
did
impact
main
conclusions
study.
Conclusions
suggests
minimal
supports
use
preprints,
same
critical
evaluation
on
applied
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Ноя. 27, 2024
Monkeypox
(Mpox)
is
a
growing
public
health
concern,
with
complex
interactions
within
host
systems
contributing
to
its
impact.
This
study
employs
multi-omics
approaches
uncover
therapeutic
targets
and
potential
drug
repurposing
opportunities
better
understand
Mpox's
molecular
pathogenesis.
We
developed
an
in
silico
host-pathogen
interaction
(HPI)
network
applied
weighted
gene
co-expression
analysis
(WGCNA)
explore
between
Mpox
proteins.
Subtype-specific
protein-protein
networks
were
constructed,
key
modules
from
the
HPI
WGCNA
integrated
identify
significant
To
predict
upstream
signaling
pathways
transcription
factors,
we
used
eXpression2Kinases
ChIP-X
Enrichment
Analysis.
The
multi-Steiner
trees
method
was
compare
our
findings
those
FDA-approved
antiviral
drugs.
Analysis
of
55
differentially
expressed
genes
infection
revealed
11
kinases
15
factors
as
regulators.
identified
16
targets,
categorized
into
8
proviral
(ESR2,
ERK1,
ERK2,
P38,
JNK1,
CDK4,
GSK3B,
STAT3)
designated
for
inhibition,
(IKKA,
HDAC1,
HIPK2,
TF65,
CSK21,
ESR2,
GSK3B)
activation.
Proviral
are
involved
AKT,
Wnt,
STAT3
pathways,
while
impact
AP-1,
NF-κB,
apoptosis,
IFN
pathways.
Promising
candidates
identified,
including
kinase
inhibitors,
steroid
hormone
receptor
agonists,
notably
Niclosamide.
enhances
understanding
by
identifying
repurposable
drugs,
providing
valuable
framework
developing
new
treatments.
Biosafety and Health,
Год журнала:
2024,
Номер
6(3), С. 186 - 190
Опубликована: Май 1, 2024
Mpox
is
a
zoonotic
infectious
disease
caused
by
the
mpox
virus
(MPXV).
Historically,
majority
of
cases
have
been
documented
in
Central
Africa.
However,
since
May
2022,
there
has
notable
rise
reported
from
regions
beyond
Currently,
over
110
countries
spanning
Europe,
North
America,
South
Asia,
and
other
territories
infections.
This
report
details
case
involving
patient
who
identifies
as
man
sex
with
men
(MSM)
concurrently
infected
MPXV,
human
immunodeficiency
type
1
(HIV-1),
Pneumocystis
jiroveci,
well
extensively
drug-resistant
tuberculosis
(XDR-TB).
had
also
received
vaccination
for
smallpox
past.
Additionally,
we
provide
photographic
documentation
charting
progression
dermatological
manifestations
associated
mpox.
highlights
significance
sexual
intercourse
crucial
mode
transmission
The
rapid
widespread
dissemination
MPXV
across
various
regions,
especially
among
MSM
communities,
underscores
importance
enhancing
preventive
education
efforts
targeted
at
high-risk
populations.
Biosafety and Health,
Год журнала:
2024,
Номер
6(5), С. 286 - 297
Опубликована: Авг. 23, 2024
The
vaccinia
virus
Tiantan
(VTT)
is
widely
utilized
as
a
smallpox
vaccine
in
China
and
holds
significant
importance
the
prevention
of
diseases
stemming
from
poxvirus
infections.
Nevertheless,
few
studies
have
investigated
influence
VTT
infection
on
host
gene
expression.
In
this
study,
we
constructed
time
series
transcriptomic
profiles
HeLa
cells
infected
with
both
western
reserve
(WR)
strains.
We
observed
similar
patterns
viral
expression,
while
expression
levels
genes
varied
between
two
There
was
an
immediate
repression
particularly
associated
oxidative
phosphorylation.
Conversely,
involved
nerve
growth
factor
(NGF)-stimulated
transcription
were
significantly
activated.
upregulation
linked
to
ribonucleic
acid
(RNA)-induced
silencing
complex
(RISC)
suggested
potential
role
for
posttranscriptional
regulation
interaction
host.
later
stages
infection,
pathways
such
extracellular
matrix
organization,
neutrophil
degranulation,
complement
interferon
responses,
translation,
programmed
cell
death
are
largely
inhibited.
A
number
exhibit
correlations
changes
genes.
that
negatively
correlated
mainly
enriched
translation
response
infection.
This
study
contributes
advancing
our
understanding
dynamics
host,
improving
application
VTTs
facilitating
development
effective
vaccines
against
monkeypox.
Research Integrity and Peer Review,
Год журнала:
2024,
Номер
9(1)
Опубликована: Окт. 6, 2024
Abstract
Background
Preprints
are
scientific
articles
that
have
not
undergone
the
peer-review
process.
They
allow
latest
evidence
to
be
rapidly
shared,
however
it
is
unclear
whether
they
can
confidently
used
for
decision-making
during
a
public
health
emergency.
This
study
aimed
compare
data
and
quality
of
preprints
released
first
four
months
2022
mpox
outbreak
their
published
versions.
Methods
Eligible
(
n
=
76)
posted
between
May
August
were
identified
through
an
established
literature
database
followed
July
2024
changes
in
publication
status.
Quality
studies
was
assessed
by
two
independent
reviewers
evaluate
quality,
using
validated
tools
available
design
33).
Tools
included
Newcastle-Ottawa
Scale;
Assessment
Diagnostic
Accuracy
Studies
2
(QUADAS-2);
JBI
Critical
Appraisal
Checklists.
The
questions
each
tool
led
overall
assessment
high
(no
concerns
with
design,
conduct,
and/or
analysis),
moderate
(minor
concerns)
or
low
(several
concerns).
Changes
(e.g.
methods,
outcomes,
results)
preprint-published
pairs
60)
one
reviewer
verified
second.
Results
versions
could
evaluated
25
pairs)
mostly
as
quality.
Minimal
no
change
from
preprint
identified:
all
observational
(10/10),
most
case
series
(6/7)
surveillance
analyses
(3/3)
had
while
some
diagnostic
test
accuracy
(3/5)
improved
worsened
scores.
Among
60),
outcomes
often
added
version
(58%)
less
commonly
removed
(18%).
Numerical
results
changed
53%
studies,
these
(22/32)
minor
did
impact
main
conclusions
study.
Conclusions
suggests
minimal
supports
use
preprints,
same
critical
evaluation
on
applied
