Spatial and Genetic Diversity of Clinical Isolates of Blastocystis in Italy: A Network Analysis

Pathogens, Год журнала: 2025, Номер 14(2), С. 139 - 139

Опубликована: Фев. 3, 2025

Blastocystis is a common intestinal protist with global distribution, frequently found in humans and various animals. Despite its prevalence, role human health remains debated, oscillating between being harmless commensal potential pathogen. It has also been associated gastrointestinal disorders such as irritable bowel syndrome (IBS) inflammatory disease (IBD). In Italy, the genetic spatial diversity of understudied, despite country’s diverse urbanized environmental landscapes. This study investigates haplotypic clinical isolates across two different Italian regions, an emphasis on subtype distribution variation. Using network-based haplotype analysis, reveals heterogeneous ST4 (47.3%) most prevalent, followed by ST3 (20%), ST1 (16.4%), ST2 (12.7%), ST6 (1.8%) ST7 (1.8%). The overall infection rate detected from symptomatic patients 9.75%. Notably, shows limited variation, suggesting more stable population structure that potentially linked to human-adapted lineage. contrast, exhibit greater diversity, likely due ongoing zoonotic transmission. These findings enhance our understanding epidemiology Italy underscore need for further research pathogenic transmission dynamics.

Язык: Английский

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Targeted-Amplicon NGS for Blastocystis sp. in Shepherd Dogs of Portugal Discriminates Co-Colonization with Multiple Zoonotic Subtypes

Veterinary Sciences, Год журнала: 2025, Номер 12(4), С. 325 - 325

Опубликована: Апрель 2, 2025

Research on endoparasites in pet dogs has been growing, but shepherd have largely overlooked. These frequently share close proximity not only with sheep, which are reservoirs of zoonotic subtypes Blastocystis, also their owners. This contact increases the potential for to act as intermediates transmission Blastocystis. To clarify role these this parasite, study investigated presence Blastocystis dogs. Stool samples from Portuguese were analyzed using SYBR-Green-based real-time PCR and melting curve analysis followed by targeted-amplicon NGS mixed infections detection. Our results revealed a 60% occurrence sp. dog stools frequent identification ST1–ST4 ST14. Additionally, we observed subtype diversity within individual dogs, suggesting cross-species between livestock humans.

Язык: Английский

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Dietary profiles of wild carnivores and Blastocystis occurrence: The case of the endangered Iberian lynx (Lynx pardinus) and systematic review

Research in Veterinary Science, Год журнала: 2024, Номер 184, С. 105518 - 105518

Опубликована: Дек. 26, 2024

Язык: Английский

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Metabarcoding in gut protozoology

Trends in Parasitology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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