Compartmentalised mucosal and blood immunity to SARS-CoV-2 associated with high seroprevalence before Delta wave in Africa DOI Creative Commons
Ya Jankey Jagne, Dawda Jobe, Alansana Darboe

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Abstract Background The reported number of SARS-CoV-2 cases and deaths are lower in Africa compared to many high-income countries. However, African cohorts, detailed characterisation mucosal T cell immunity limited. We assessed SARS-CoV-2-specific immune landscape Gambia pre-Delta variant July 2021. Methods A cross-sectional assessment 349 unvaccinated individuals from 52 Gambian households was performed between March - June spike (S) nucleocapsid (N) specific binding antibodies were measured by ELISA, variant-specific serum neutralizing-antibodies (NAb) viral pseudotype assays nasal fluid IgA mesoscale discovery assay. T-cell responses evaluated using ELISpot Results show that adjusted seroprevalence anti-Spike 56.7% (95% confidence interval (CI) 49.0–64.0), which children < 5 years (26.2%, 13.9–43.8) 5–17 (46.4%, 36.2–56.7) adults 18–49 (78.4%, 68.8–85.8). In spike-seropositive individuals, NAb titres highest Alpha (median IC50 110), with 27% showing pre-existing Delta > 1:50. Whilst significantly higher 34% spike-seronegative showed reactivity one or more antigen pools. Strong correlations within T-cell, IgA, observed. Conclusion High The-Gambia induced blood immunity, reducing Omicron impact. Children relatively protected infection. seronegative may indicate either pre-pandemic cross-reactivity a dominated response infection absent poor humoral responses.

Язык: Английский

Dynamics of SARS-CoV-2 Immunoglobulin G Antibody Among Hospitalized Patients and Healthcare Workers During the Delta Wave in Bangladesh DOI Open Access
Md. Abdul Matin, Chowdhury Adnan Sami,

Md Ashraful Hassan Anjan

и другие.

Cureus, Год журнала: 2025, Номер unknown

Опубликована: Апрель 13, 2025

A reliable marker of COVID-19 past infection is the anti-spike IgG antibody (Ab) titer SARS-CoV-2 virus, which may be identified even one year from diagnosis. Serology can effective in diagnosing asymptomatic and patients with negative reverse transcription polymerase chain reaction. Exploring these dynamics persistence antibodies vital to understanding disease process. We designed this observational study compare seroconversion status healthy healthcare workers. The total number participants was 272, including 58 hospitalized (recovered) patients, 153 workers (HCWs), 61 control populations community. 404 samples were taken for measurements using enzyme-linked immunosorbent assay method. Among 58.6% men, male-to-female ratio 1:1.41. All had three months Ab titer. median (interquartile range) optical density values severe group on day 0, as well during fourth, eighth, twelfth weeks, 9.25 (6.01-11.893), 8.95 (5.52-12.42), 13.19 (12.26-13.26), 12.99 (9.23-13.24), respectively. In comparison, nonsevere exhibited 4.19 (3.08-9.89), 6.99 (4.32-9.75), 8.65 (5.83-13.08), 12.48 (7.88-13.16), However, seroprevalence higher (83.3%); HCWs, it 54.9%. Although antispike titers, no difference titers seen among age, gender, body mass index, or diabetes subgroups. observed up cases COVID-19-recovered whereas high HCWs Delta wave.

Язык: Английский

Процитировано

0
Compartmentalised mucosal and blood immunity to SARS-CoV-2 is associated with high seroprevalence before the Delta wave in Africa DOI Creative Commons
Ya Jankey Jagne, Dawda Jobe, Alansana Darboe

и другие.

Communications Medicine, Год журнала: 2025, Номер 5(1)

Опубликована: Май 16, 2025

The reported number of SARS-CoV-2 cases and deaths are lower in Africa compared to many high-income countries. However, African cohorts, detailed characterisation mucosal T cell immunity limited. We assessed the SARS-CoV-2-specific immune landscape Gambia during presence pre-Delta variant July 2021. A cross-sectional assessment 349 unvaccinated individuals from 52 Gambian households was performed between March-June spike (S) nucleocapsid (N) specific binding antibodies were measured by ELISA, variant-specific serum neutralizing-antibodies (NAb) viral pseudotype assays nasal fluid IgA mesoscale discovery assay. T-cell responses evaluated using ELISpot show that adjusted anti-Spike antibody seroprevalence is 56.7% (95% confidence interval (CI) 49.0-64.0), with rates children <5 years (26.2%, 13.9-43.8) 5-17 (46.4%, 36.2-56.7) adults 18-49 (78.4%, 68.8-85.8). Among spike-seropositive individuals, NAb titres highest against Alpha (median IC50 110), 27% showing pre-existing Delta >1:50. higher although 34% spike-seronegative at least one antigen pool. observe strong correlations within T-cell, IgA, responses. High The-Gambia induce blood immunity, reducing Omicron impact. Children relatively protected infection. seronegative may indicate either pre-pandemic cross-reactivity or a dominated response infection absent poor humoral

Язык: Английский

Процитировано

0
Immunogenicity of a third dose with mRNA-vaccines in the ChAdOx1-S/BNT162b2 vaccination regimen against SARS-CoV-2 variants. DOI Creative Commons
Javier García‐Pérez, Alberto M. Borobia, Mayte Pérez‐Olmeda

и другие.

iScience, Год журнала: 2024, Номер 27(9), С. 110728 - 110728

Опубликована: Авг. 14, 2024

CombiVacS study has demonstrated a strong immune response of the heterologous ChAdOx1-S/BNT162b2 vaccine combination. The primary outcomes were to assess humoral against SARS-CoV-2, 28 days after third dose mRNA vaccine, in subjects that received previous prime-boost scheme with ChAdOx1-S/BNT162b2. Secondary extended 3 and 6 months. mRNA-1273 naive participants previously vaccinated regimen reached higher neutralizing antibodies titers variants concern Delta BA.1 lineage Omicron compared those receiving BNT162b2 at day 28. These differences between arms observed ancestral variant G614 90. Suboptimal was BQ.1.1, XBB.1.5/XBB.1.9, JN.1 relevant proportion individuals 180 dose, even asymptomatic breakthrough infections. EudraCT (2021-001978-37); ClinicalTrials.gov (NCT04860739).

Язык: Английский

Процитировано

0
Compartmentalised mucosal and blood immunity to SARS-CoV-2 associated with high seroprevalence before Delta wave in Africa DOI Creative Commons
Ya Jankey Jagne, Dawda Jobe, Alansana Darboe

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Abstract Background The reported number of SARS-CoV-2 cases and deaths are lower in Africa compared to many high-income countries. However, African cohorts, detailed characterisation mucosal T cell immunity limited. We assessed SARS-CoV-2-specific immune landscape Gambia pre-Delta variant July 2021. Methods A cross-sectional assessment 349 unvaccinated individuals from 52 Gambian households was performed between March - June spike (S) nucleocapsid (N) specific binding antibodies were measured by ELISA, variant-specific serum neutralizing-antibodies (NAb) viral pseudotype assays nasal fluid IgA mesoscale discovery assay. T-cell responses evaluated using ELISpot Results show that adjusted seroprevalence anti-Spike 56.7% (95% confidence interval (CI) 49.0–64.0), which children < 5 years (26.2%, 13.9–43.8) 5–17 (46.4%, 36.2–56.7) adults 18–49 (78.4%, 68.8–85.8). In spike-seropositive individuals, NAb titres highest Alpha (median IC50 110), with 27% showing pre-existing Delta > 1:50. Whilst significantly higher 34% spike-seronegative showed reactivity one or more antigen pools. Strong correlations within T-cell, IgA, observed. Conclusion High The-Gambia induced blood immunity, reducing Omicron impact. Children relatively protected infection. seronegative may indicate either pre-pandemic cross-reactivity a dominated response infection absent poor humoral responses.

Язык: Английский

Процитировано

0