Multi-omics analysis reveals the impact of influenza a virus host adaptation on immune signatures in pig tracheal tissue

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Авг. 16, 2024

Introduction Influenza A virus (IAV) infection is a global respiratory disease, which annually leads to 3-5 million cases of severe illness, resulting in 290,000-650,000 deaths. Additionally, during the past century, four IAV pandemics have claimed millions human lives. The epithelial lining trachea plays vital role infection, both as point viral entry and replication well antiviral immune response. Tracheal tissue generally inaccessible from patients, makes animal models crucial for study tracheal host Method In this study, pigs were inoculated with swine- or human-adapted H1N1 gain insight into how adaptation shapes innate response infection. In-depth multi-omics analysis (global proteomics RNA sequencing) upper lower was conducted, results validated by microfluidic qPCR. subset samples selected histopathological examination. Results classical induced after either upregulation genes higher abundance proteins associated recognition, accompanied significant induction interferon stimulated corresponding concentrations. Infection swine-adapted much stronger compared strain trachea, could be consequence load degree inflammation. Discussion Central components JAK-STAT pathway, apoptosis, pyrimidine metabolism, cytoskeleton significantly altered depending on might relevant mechanisms relation immunity against putative zoonotic IAV. Based our findings, we hypothesize that adaptation, evolve modulate important cell elements favor infectivity replication.

Язык: Английский

Genetic evolution, phylodynamic and phylogeographic of H5Ny AIVs in mammals

Animals and zoonoses., Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский