Science in One Health,
Год журнала:
2024,
Номер
3, С. 100080 - 100080
Опубликована: Янв. 1, 2024
The
present
comprehensive
protocol
is
focused
on
the
detection
of
pathogenic
enteric
RNA
viruses,
explicitly
focusing
norovirus
genogroup
Ⅱ
(GⅡ),
astrovirus,
rotavirus,
Aichi
virus,
sapovirus,
hepatitis
A
and
E
viruses
in
wastewater
treatment
plants
through
droplet
digital
PCR
(ddPCR).
Enteric
are
significant
public
health
concern,
as
they
leading
cause
diseases
like
gastroenteritis.
Regular
monitoring
environmental
samples,
particularly
from
plants,
crucial
for
early
control
these
viruses.
This
research
aims
to
improve
understanding
prevalence
dynamics
urban
India
will
serve
a
model
similar
studies
other
regions.
Our
protocol's
objective
establish
novel
ddPCR-based
methodology
molecular
characterization
samples
sourced
Bhopal,
India.
assay
capable
accurately
quantifying
virus
concentrations
without
standard
curves,
minimizing
extensive
optimization,
enhancing
sensitivity
precision,
especially
low-abundance
targets.
Abstract
Background
Periodic
bioinformatics-based
screening
of
wastewater
for
assessing
the
diversity
potential
human
viral
pathogens
circulating
in
a
given
community
may
help
to
identify
novel
or
potentially
emerging
infectious
diseases.
Any
identified
contigs
related
viruses
should
be
confirmed
with
targeted
and
clinical
testing.
Results
During
COVID-19
pandemic,
untreated
samples
were
collected
1-year
period
from
Great
Lakes
Water
Authority
Wastewater
Treatment
Facility
Detroit,
MI,
USA,
population
both
centralized
interceptor
sites
localized
neighborhood
sewersheds
was
investigated.
Clinical
cases
diseases
caused
by
tabulated
compared
data
monitoring.
In
addition
Betacoronavirus
,
comparison
using
assembled
against
custom
Swiss-Prot
virus
database
indicated
prevalence
other
pathogenic
genera,
including:
Orthopoxvirus
Rhadinovirus,
Parapoxvirus
Varicellovirus,
Hepatovirus,
Simplexvirus,
Bocaparvovirus,
Molluscipoxvirus,
Parechovirus,
Roseolovirus,
Lymphocryptovirus,
Alphavirus,
Spumavirus,
Lentivirus,
Deltaretrovirus,
Enterovirus,
Kobuvirus,
Gammaretrovirus,
Cardiovirus,
Erythroparvovirus,
Salivirus,
Rubivirus,
Orthohepevirus,
Cytomegalovirus,
Norovirus,
Mamastrovirus
.
Four
nearly
complete
genomes
recovered
Astrovirus,
Norovirus
Betapolyomavirus
genera
species
identified.
Conclusions
The
presented
findings
are
primarily
at
genus
level
can
serve
as
preliminary
“screening”
tool
that
indication
initiate
further
testing
confirmation
presence
associated
disease.
Integrating
innovative
environmental
microbiology
technologies
like
metagenomic
sequencing
epidemiology
offers
significant
opportunity
improve
monitoring
of,
predictive
intelligence
for,
viruses,
wastewater.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 30, 2025
The
CRISPR-based
detection
methods
have
been
widely
applied,
yet
they
remain
limited
by
the
non-universal
nature
of
one-pot
diagnostic
approaches.
Here,
we
report
a
universal
fluorescent
method
for
epidemic
pathogens,
delivering
results
within
15-20
min.
This
uses
heparin
sodium
to
precisely
tunes
cis-cleavage
capability
Cas12
via
interference
with
Cas12a-crRNA
binding
process,
thereby
generating
significant
fluorescence
due
accumulation
isothermal
amplification
products.
Additionally,
this
assay
accommodates
both
classic
and
suboptimal
PAMs,
as
well
various
Cas12a
subtypes
such
LbCas12a,
AsCas12a,
AapCas12b.
Such
robust
demonstrates
sensitivity
specificity
exceeding
95%
in
monkeypox
pseudovirus,
influenza
A
virus,
SARS-CoV-2
from
saliva
or
wastewater
samples,
when
compared
qPCR
RT-qPCR.
Moreover,
cost
per
thousand
is
$0.01
$0.04
only.
Collectively,
fast
approach
based
on
offers
potential
possibilities
point-of-care
testing.
Engineering,
Год журнала:
2024,
Номер
40, С. 70 - 77
Опубликована: Март 15, 2024
Wastewater
surveillance
can
leverage
its
wide
coverage,
population-based
sampling,
and
high
monitoring
frequency
to
capture
citywide
pandemic
trends
independent
of
clinical
surveillance.
Here
we
conducted
a
9-month
daily
wastewater
for
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
from
12
treatment
plants
(WWTPs),
covering
approximately
80%
the
population,
monitor
infection
dynamics
in
Hong
Kong.
We
found
that
SARS-CoV-2
virus
concentration
was
correlated
with
number
reported
cases
reached
two
peaks
three
days
earlier
during
study
period.
In
addition,
different
methods
were
established
estimate
prevalence/incidence
rates
measurements.
The
estimated
results
consistent
findings
programmes
(rapid
antigen
test
(RAT)
serology
surveillance),
but
higher
than
by
Centre
Health
Protection
(CHP)
Moreover,
effective
reproductive
(Rt)
measurements
reflect
both
regional
transmission
dynamics.
Our
demonstrate
large-scale
intensive
WWTPs
provides
cost-effective
timely
public
health
information,
especially
when
is
inadequate
costly.
This
approach
also
insights
into
at
spatiotemporal
resolutions,
facilitating
formulation
control
policies
targeted
resource
allocation.
Deleted Journal,
Год журнала:
2024,
Номер
6(15), С. 332 - 338
Опубликована: Янв. 1, 2024
Introduction
The
emergence
of
the
new
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
Omicron
sublineage,
BA.2.86,
has
sparked
global
public
health
concerns
for
its
potential
heightened
transmissibility
and
immune
evasion.
Utilizing
data
from
Shenzhen's
city-wide
wastewater
surveillance
system,
we
highlight
presence
BA.2.86
lineage
in
Shenzhen.
Methods
A
mediator
probe
polymerase
chain
reaction
(PCR)
assay
was
developed
to
detect
by
targeting
a
specific
mutation
(Spike:
A264D).
Between
September
19
December
10,
2023,
781
samples
38
treatment
plants
(WWTPs)
9
pump
stations
ten
districts
Shenzhen
were
examined.
Through
multiple
short-amplicon
sequencing,
three
positive
identified.
Results
identified
Futian
Nanshan
on
2,
2023.
From
total
21
BA.2.86-positive
found
across
6
(Futian,
Nanshan,
Longhua,
Baoan,
Longgang,
Luohu)
weighted
average
viral
load
43.5
copies/L
increased
219.8
4,
then
decreased
approximately
100
6,
8,
10.
Conclusions
PCR
assay,
designed
swift
detection
low
concentrations
samples,
shows
promise
detecting
different
SARS-CoV-2
variants.
Wastewater
could
serve
as
an
early
system
promptly
identifying
variants
they
emerge.
