ACS ES&T Engineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 8, 2024
Thiosulfate-assisted
anaerobic
fermentation
(AF)
effectively
converts
waste
activated
sludge
into
high-value
products
(e.g.,
short-chain
fatty
acids
(SCFAs)).
However,
the
roles
of
thiosulfate
in
organics
transformation,
electron
transfer,
and
microbial
interactions
within
AF
systems
are
not
fully
understood,
especially
under
long-term
operations.
In
this
study,
an
88
day
experiment
was
conducted
to
address
knowledge
gap.
The
results
indicated
average
SCFA
yield
3625.1
mg
COD/L
acetate
proportion
49.4%
with
a
dosage
600
S/L.
Model
organic
degradation
tests
revealed
that
functioned
as
acceptor,
facilitating
NAD+/NADH
stimulating
expression
protein
complexes
like
cytochrome
c
enhance
transport,
lowering
thermodynamic
barriers
propionate
butyrate
(ΔG1propionate
=
−335.0
kJ/mol;
ΔG2butyrate
−113.8
kJ/mol).
Molecular
ecological
networks
analysis
showed
strengthened
cooperative
relationships
among
biomarkers
hydrolytic
bacteria
(i.e.,
Proteiniphilum,
UBA5851),
acidogenic
UBA4179),
sulfur
reducers
JAEUSI01).
Functional
gene
using
random
forest
confirmed
upregulated
key
genes
2-oxoacid
ferredoxin
oxidoreductase)
associated
transfer
metabolism.
This
study
deepens
our
understanding
thiosulfate,
strengthening
cooperation
systems.
Microorganisms,
Год журнала:
2025,
Номер
13(1), С. 93 - 93
Опубликована: Янв. 6, 2025
The
adhesion
between
dibenzofuran
(DF)
and
degrading
bacteria
is
the
first
step
of
DF
biodegradation
affects
efficient
degradation
DF.
However,
their
mechanism
at
molecular
level
remains
unclear.
Therefore,
this
study
examined
adhesive
behaviors
mechanisms
Rhodococcus
sp.
strain
p52
upon
exposure
to
results
showed
that
mediated
by
extracellular
polymeric
substances
(EPSs).
Compared
with
sodium
acetate
as
a
carbon
source,
percentages
glucose
proteins
related
electron
transfer,
toxin-antitoxin,
stress
responses
were
elevated,
which
analyzed
polysaccharide
composition
proteomics,
contents
polysaccharides
increased.
Moreover,
biofilm
analysis
suggested
an
increase
in
EPS
content,
change
components
increased
yield
promoted
loose
porous
aggregation
bacteria;
caused
specific
surface
area
contact
characteristics
indicated
production
reduced
absolute
value
zeta
potential
hydrophobicity
p52,
was
beneficial
for
These
findings
help
us
enhance
understanding
bioremediation
polycyclic
aromatic
hydrocarbons
bacteria.
