Journal of Hazardous Materials, Год журнала: 2024, Номер unknown, С. 135770 - 135770
Опубликована: Сен. 1, 2024
Язык: Английский
Journal of Hazardous Materials, Год журнала: 2024, Номер unknown, С. 135770 - 135770
Опубликована: Сен. 1, 2024
Язык: Английский
Environmental Chemistry Letters, Год журнала: 2024, Номер 22(5), С. 2241 - 2255
Опубликована: Июнь 26, 2024
Язык: Английский
Процитировано
4Journal of environmental chemical engineering, Год журнала: 2025, Номер unknown, С. 115761 - 115761
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0Journal of Hazardous Materials, Год журнала: 2025, Номер unknown, С. 138004 - 138004
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Апрель 17, 2025
Nanoplastics (NPs) are prevalent in the environment, posing risks to ecosystems and human health. While research into their effects on bacterial activity has increased, mechanisms underlying NP-bacteria interactions─specifically whether NPs penetrate cells or adhere cell surface─remain poorly understood. This knowledge gap largely stems from absence of quantitative analytical methods. Herein, we developed a novel approach combining lysozyme treatment with pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) differentiate between intracellular wall-bound Escherichia coli (E. coli) quantitatively. The method involves selective removal wall using lysozyme, protein corona-induced extraction enrich NPs, hydrogen peroxide digestion eliminate protoplast interference before Py-GC/MS analysis. Validation europium (Eu)-labeled quantified by inductively coupled plasma mass (ICP-MS), confirmed method's accuracy reliability. Using this approach, found that after NP exposure, only small fraction (9.6-10.5%) penetrated E. cells, while majority (36.9-63.8%) adhered surface. Transmission electron microscopy further corroborated these findings. Consequently, work provides robust tool for quantification uptake biodistribution systems, advancing our understanding NP-microorganism interactions environmental implications.
Язык: Английский
Процитировано
0Journal of Hazardous Materials, Год журнала: 2024, Номер unknown, С. 135770 - 135770
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
2