Wastewater surveillance to infer COVID-19 transmission: A systematic review

The Science of The Total Environment, Год журнала: 2021, Номер 804, С. 150060 - 150060

Опубликована: Сен. 8, 2021

Successful detection of SARS-COV-2 in wastewater suggests the potential utility wastewater-based epidemiology (WBE) for COVID-19 community surveillance. This systematic review aims to assess performance surveillance as early warning system transmission. A search was conducted PubMed, Medline, Embase and WBE Consortium Registry according PRISMA guidelines relevant articles published until 31st July 2021. Relevant data were extracted summarized. Quality each paper assessed using an assessment tool adapted from Bilotta et al.'s environmental science. Of 763 studies identified, 92 distributed across 34 countries shortlisted qualitative synthesis. total 26,197 samples collected between January 2020 May 2021 various locations serving population ranging 321 11,400,000 inhabitants. Overall sample positivity moderate at 29.2% all examined settings with spike (S) gene having maximum rate positive detections nucleocapsid (N) being most targeted. Wastewater signals preceded confirmed cases by up 63 days, 13 reporting before first detected community. At least 50 reported association viral load cases. While cannot replace large-scale diagnostic testing, it can complement clinical providing signs transmission more active public health responses. However, standardized validated methods are required along risk analysis modelling understand dynamics outbreaks.

Язык: Английский

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Understanding and managing uncertainty and variability for wastewater monitoring beyond the pandemic: Lessons learned from the United Kingdom national COVID-19 surveillance programmes

Journal of Hazardous Materials, Год журнала: 2021, Номер 424, С. 127456 - 127456

Опубликована: Окт. 9, 2021

The COVID-19 pandemic has put unprecedented pressure on public health resources around the world. From adversity, opportunities have arisen to measure state and dynamics of human disease at a scale not seen before. In United Kingdom, evidence that wastewater could be used monitor SARS-CoV-2 virus prompted development National surveillance programmes. pace this work proven unique in monitoring national level, demonstrating importance wastewater-based epidemiology (WBE) for protection. Beyond COVID-19, it can provide additional value informing range biological chemical markers health. A discussion measurement uncertainty associated with wastewater, focusing lessons-learned from UK programmes is presented, showing sources impacting quality interpretation data decision-making, are varied complex. While some factors remain poorly understood, we present approaches taken by manage mitigate more tractable uncertainty. This provides platform integrate management into WBE activities as part global One Health initiatives beyond pandemic.

Язык: Английский

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An analysis of 45 large-scale wastewater sites in England to estimate SARS-CoV-2 community prevalence

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Июль 25, 2022

Accurate surveillance of the COVID-19 pandemic can be weakened by under-reporting cases, particularly due to asymptomatic or pre-symptomatic infections, resulting in bias. Quantification SARS-CoV-2 RNA wastewater used infer infection prevalence, but uncertainty sensitivity and considerable variability has meant that accurate measurement remains elusive. Here, we use data from 45 sewage sites England, covering 31% population, estimate prevalence within 1.1% estimates representative surveys (with 95% confidence). Using machine learning phenomenological models, show differences between sampled sites, flow rate, influence estimation require careful interpretation. We find signals appear 4-5 days earlier comparison clinical testing are coincident with suggesting a leading indicator for symptomatic viral infections. Surveillance viruses complements strengthens surveillance, significant implications public health.

Язык: Английский

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Factors influencing SARS-CoV-2 RNA concentrations in wastewater up to the sampling stage: A systematic review

The Science of The Total Environment, Год журнала: 2022, Номер 820, С. 153290 - 153290

Опубликована: Янв. 20, 2022

Язык: Английский

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Does normalization of SARS-CoV-2 concentrations by Pepper Mild Mottle Virus improve correlations and lead time between wastewater surveillance and clinical data in Alberta (Canada): comparing twelve SARS-CoV-2 normalization approaches

The Science of The Total Environment, Год журнала: 2022, Номер 856, С. 158964 - 158964

Опубликована: Сен. 24, 2022

Язык: Английский

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CrAssphage as an indicator of human-fecal contamination in water environment and virus reduction in wastewater treatment

Water Research, Год журнала: 2022, Номер 221, С. 118827 - 118827

Опубликована: Июль 5, 2022

Язык: Английский

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Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond

Clinical Microbiology Reviews, Год журнала: 2023, Номер 37(1)

Опубликована: Дек. 14, 2023

SUMMARY Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context of coronavirus disease 2019 (COVID-19) pandemic. The power and potential this platform technology were rapidly realized when it became evident that not only did WBS-measured SARS-CoV-2 RNA correlate strongly with COVID-19 clinical within monitored populations but also, fact, functioned as a leading indicator. Teams from across globe innovated novel approaches by which wastewater could be collected diverse sewersheds ranging treatment plants (enabling community-level surveillance) to more granular locations including individual neighborhoods high-risk buildings such long-term care facilities (LTCF). Efficient processes enabled extraction concentration highly dilute matrix. Molecular genomic tools identify, quantify, characterize its various variants adapted programs applied these mixed environmental systems. Novel data-sharing allowed information mobilized made immediately available public health government decision-makers even public, enabling evidence-informed decision-making based on local dynamics. WBS since been recognized tool transformative potential, providing near-real-time cost-effective, objective, comprehensive, inclusive data changing prevalence measured analytes space time populations. However, consequence rapid innovation hundreds teams simultaneously, tremendous heterogeneity currently exists literature. This manuscript provides state-of-the-art review established details current work underway expanding scope other infectious targets.

Язык: Английский

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Tools for interpretation of wastewater SARS-CoV-2 temporal and spatial trends demonstrated with data collected in the San Francisco Bay Area

Water Research X, Год журнала: 2021, Номер 12, С. 100111 - 100111

Опубликована: Июль 31, 2021

Wastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be integrated with COVID-19 case data to inform timely pandemic response. However, more research is needed apply and develop systematic methods interpret the true SARS-CoV-2 signal from noise introduced in wastewater samples (e.g., sewer conditions, sampling extraction methods, etc.). In this study, raw was collected weekly five sewersheds one residential facility. The concentrations of were compared geocoded clinical testing data. reliably detected (95% positivity) frozen when reported daily new cases 2.4 or per 100,000 people. To adjust variation sample fecal content, four normalization biomarkers evaluated: crAssphage, pepper mild mottle virus, Bacteroides ribosomal (rRNA), human 18S rRNA. Of these, crAssphage displayed least spatial temporal variability. Both unnormalized normalized had positive significant correlation (Kendall's Tau-b (τ)=0.43 0.38, respectively), but no biomarker strengthened Locational dependencies date associated impacted lead time trends, observed collection (versus result date) used both This study supports that trends reflect disease occurrence presents tools could applied make interpretable comparable across studies.

Язык: Английский

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Contribution of SARS-CoV-2 RNA shedding routes to RNA loads in wastewater

The Science of The Total Environment, Год журнала: 2021, Номер 806, С. 150376 - 150376

Опубликована: Сен. 16, 2021

Язык: Английский

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Comparison of five polyethylene glycol precipitation procedures for the RT-qPCR based recovery of murine hepatitis virus, bacteriophage phi6, and pepper mild mottle virus as a surrogate for SARS-CoV-2 from wastewater

The Science of The Total Environment, Год журнала: 2021, Номер 807, С. 150722 - 150722

Опубликована: Окт. 4, 2021

Polyethylene glycol (PEG) precipitation is one of the conventional methods for virus concentration. This technique has been used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. The procedures and seeded surrogate viruses were different among implementers; thus, reported whole process recovery efficiencies considerably varied studies. present study compared five PEG procedures, with operational parameters, RT-qPCR-based efficiency murine hepatitis (MHV), bacteriophage phi6, pepper mild mottle (PMMoV), molecular norovirus using 34 raw wastewater samples collected Japan. yielded significantly MHV (0.070%-2.6%) phi6 (0.071%-0.51%). observed concentration indigenous PMMoV ranged from 8.9 9.7 log (8.2 × 10

Язык: Английский

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