Applied Sciences,
Год журнала:
2024,
Номер
14(23), С. 11376 - 11376
Опубликована: Дек. 6, 2024
Rat
dental
pulp
stem
cells
(DPSCs)
can
be
used
to
elucidate
mesenchymal
cell
(MSC)
applications
in
regenerative
medicine.
However,
information
on
rat
DPSCs
during
long-term
passage,
which
could
lead
replicative
senescence,
is
limited.
In
this
study,
we
investigated
the
phenotypic
changes
after
3–26
passages
(3P–26P).
The
results
show
that
morphology
and
nuclear
size
increase
proportionally
with
passage
number.
phosphorylated
histone
H2A.X
(γ-H2A.X)
positive
(indicating
DNA
damage)
increased
significantly
earlier
than
4-Hydroxynonenal
(4-HNE)
stained
an
abundance
of
intracellular
reactive
oxygen
species).
Compared
subjected
3P
5P,
15P
showed
reduced
proliferation
despite
being
for
Ki67.
Furthermore,
growth
was
completely
arrested
26P.
senescence
markers,
senescence-associated
β-galactosidase
(SA-β-gal)
p16,
exhibited
similar
expression
patterns
were
not
correlated
those
p21
urokinase-type
plasminogen
activator
receptor
(uPAR).
Nearly
all
expressed
SA-β-gal
p16
26P,
whereas
only
half
uPAR.
These
will
contribute
understanding
characteristics
toward
are
applicable
mechanisms
related
medicine
aging.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3275 - 3275
Опубликована: Апрель 1, 2025
Neuroinflammation
is
a
complex
immune
response
triggered
by
brain
injury
or
pathological
stimuli,
and
highly
exacerbated
in
neurodegenerative
diseases.
It
plays
dual
role
the
central
nervous
system,
promoting
repair
acute
stages
while
aggravating
disease
progression
contributing
to
neuronal
loss,
synaptic
dysfunction,
glial
dysregulation
chronic
phases.
Inflammatory
responses
are
mainly
orchestrated
microglia
infiltrated
monocytes,
which,
when
dysregulated,
not
only
harm
existing
neurons,
but
also
impair
survival
differentiation
of
neural
stem
progenitor
cells
affected
regions.
Modulating
neuroinflammation
crucial
for
harnessing
its
protective
functions
minimizing
detrimental
effects.
Current
therapeutic
strategies
focus
on
fine-tuning
inflammatory
through
pharmacological
agents,
bioactive
molecules,
cell-based
therapies.
These
approaches
aim
restore
homeostasis,
support
neuroprotection,
promote
regeneration
various
neurological
disorders.
However,
animal
models
sometimes
fail
reproduce
human-specific
brain.
In
this
context,
stem-cell-derived
provide
powerful
tool
study
neuroinflammatory
mechanisms
patient-specific
physiologically
relevant
context.
facilitate
high-throughput
screening,
personalized
medicine,
development
targeted
therapies
addressing
limitations
traditional
models,
paving
way
more
effective
treatments.
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 1, 2025
Osteoarthritis
(OA)
is
a
widely
prevalent
chronic
degenerative
disease
often
associated
with
significant
pain
and
disability.
It
characterized
by
the
deterioration
of
cartilage
extracellular
matrix
(ECM),
synovial
inflammation,
subchondral
bone
remodeling.
Recent
studies
have
highlighted
pyroptosis-a
form
programmed
cell
death
triggered
inflammasome-as
key
factor
in
sustaining
inflammation.
Central
to
this
process
are
inflammatory
cytokines
interleukin-1β
(IL-1β)
interleukin-18
(IL-18),
which
play
crucial
roles
mediating
intra-articular
pyroptosis
through
NOD-like
receptor
protein
3
(NLRP3)
inflammasome.
This
paper
investigates
role
pathway
perpetuating
diseases
its
linkage
OA.
Furthermore,
it
explores
mechanisms
pyroptosis,
mediated
nuclear
κB
(NF-κB),
purinergic
P2X
ligand-gated
ion
channel
7
(P2X7R),
adenosine
monophosphate
(AMP)-activated
kinase
(AMPK),
hypoxia-inducible
factor-1α
(HIF-1α).
Additionally,
examines
interactions
among
various
cellular
components
context
These
insights
indicate
that
targeting
regulation
presents
promising
therapeutic
approach
for
prevention
treatment
OA,
offering
valuable
theoretical
perspectives
effective
management.
Systematic Reviews,
Год журнала:
2025,
Номер
14(1)
Опубликована: Апрель 5, 2025
This
study
aimed
to
systematically
evaluate
the
efficacy
of
electroacupuncture
and
manual
acupuncture
for
treating
vascular
dementia
determine
optimal
point
combination
scheme
efficacy.
The
PubMed,
Embase,
Web
Science,
Cochrane,
CNKI,
VIP,
Wanfang
electronic
databases
were
searched
up
July
2024
identify
relevant
randomized
controlled
trials.
RevMan
5.4
software
Addis
used
assess
risk
bias
each
study,
subgroup
classifications,
conduct
meta-analyses.
A
total
29
RCTs
involving
659
animals
ultimately
included.
meta-analysis
results
revealed
that
treatment
had
a
significant
effect
compared
with
model
group
[mean
difference
(MD)
=
-
21.68,
95%
confidence
interval
(CI)
(-
25.77,
17.59),
P
<
0.00001].
Manual
demonstrated
better
than
did
[MD
0.42,
CI
12.72,
12.27)].
Among
different
combinations,
Baihui
(GV20)
+
Dazhui
(GV14)
yielded
best
23.03,
30.02,
16.04),
Compared
other
protocols,
experiment
conducted
by
Caiyu
Peng
et
al.
exhibited
superior
24.96,
92.68,
40.76)].
Acupuncture
significantly
improves
cognitive
function
in
rats
dementia.
is
more
effective
electroacupuncture.
at
GV20
GV14
yields
results.
was
observed
when
two-vessel
occlusion
(2VO)
230
±
10
g
SD
rats;
Mingmen
(GV4),
(GV14),
Fengfu
(GV16),
(GV20),
Shenting
(GV24),
Shuigou
(GV26),
Neiguan
(PC6),
Dalin
(PC7),
Laogong
(PC8)
acupoints
selected;
reinforcing
reducing
methods
30
min
per
day
14
days.
PROSPERO
CRD42024551402.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Авг. 5, 2024
Urate
nephropathy,
a
common
complication
of
hyperuricemia,
has
garnered
increasing
attention
worldwide.
However,
the
exact
pathogenesis
this
condition
remains
unclear.
Currently,
inflammation
is
widely
accepted
as
key
factor
in
urate
nephropathy.
Therefore,
aim
study
was
to
elucidate
interaction
lincRNA-p21/AIF-1/CMPK2/NLRP3
via
exosomes
This
evaluated
effect
using
clinical
data
collected
from
patients
with
nephropathy
and
human
renal
tubular
epithelial
cells
(HK2)
cultured
different
concentrations
urate.
In
research
section,
level
lincRNA-p21/AIF-1
urine
hyperuricemia
or
found
be
increased,
particularly
vitro
exosomes,
inflammation,
autophagy,
apoptosis
increased
HK2
induced
by
Additionally,
expression
lincRNA-p21,
AIF-1,
CMPK2,
NLRP3
upregulated
cells.
Furthermore,
manipulating
activity
through
overexpression
interference
vectors
regulated
conclusion,
pathway
contributed
cell
exosomes.
specific
might
serve
novel
biomarkers
for
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 18, 2024
Abstract
Macrophage‐mediated
inflammatory
responses
including
pyroptosis
are
involved
in
the
pathogenesis
of
sepsis
and
acute
lung
injury
(ALI),
for
which
there
currently
no
effective
therapeutic
treatments.
The
natural
product
(+)‐Balasubramide
is
an
eight‐membered
lactam
compound
extracted
from
leaves
Sri
Lanka
plant
Clausena
Indica
has
shown
anti‐inflammatory
activities,
but
its
poor
pharmacokinetic
properties
limit
further
application
ALI.
In
this
study,
a
(+)3C‐20
discovered
with
improved
both
activity
series
derivatives.
exhibits
markedly
enhanced
inhibitory
effect
against
LPS‐induced
expressions
pro‐inflammatory
factors
mouse
macrophages
human
PBMCs
ALI
patients
shows
preferable
tissue
distribution
mice.
remarkably
attenuates
through
tissue‐specific
actions.
Mechanistically,
chemical
proteomics
study
that
directly
binds
to
mitochondrial
VDAC1
inhibits
oligomerization
block
mtDNA
release,
preventing
NLRP3
inflammasome
activation.
These
findings
identify
as
novel
inhibitor
promising
potential
associated
inflammation.
Proteomes,
Год журнала:
2024,
Номер
12(4), С. 35 - 35
Опубликована: Ноя. 27, 2024
As
the
primary
innate
immune
cells
of
brain,
microglia
play
a
key
role
in
various
homeostatic
and
disease-related
processes.
To
carry
out
their
numerous
functions,
adopt
wide
range
phenotypic
states.
The
proteomic
landscape
represents
more
accurate
molecular
representation
these
phenotypes;
however,
present
unique
challenges
for
analysis.
This
study
implemented
streamlined
liquid-
gas-phase
fractionation
method
with
data-dependent
acquisition
(DDA)
parallel
accumulation–serial
fragmentation
(PASEF)
analysis
on
TIMS-TOF
instrument
to
compile
comprehensive
protein
library
obtained
from
adult-derived,
immortalized
mouse
low
starting
material
(10
µg).
empirical
consisted
9140
microglial
proteins
was
utilized
identify
an
average
7264
proteins/run
single-shot,
data-independent
(DIA)-based
cell
lysate
digest
(200
ng).
Additionally,
predicted
facilitated
identification
7519
same
DIA
data,
revealing
complementary
coverage
compared
collectively
increasing
approximately
8000
proteins.
Importantly,
several
microglia-relevant
pathways
were
uniquely
identified
approach.
Overall,
we
report
simplified,
reproducible
approach
address
proteome
complexity
using
sample
input
show
importance
optimization
this
phenotypically
diverse
type.
Applied Sciences,
Год журнала:
2024,
Номер
14(23), С. 11376 - 11376
Опубликована: Дек. 6, 2024
Rat
dental
pulp
stem
cells
(DPSCs)
can
be
used
to
elucidate
mesenchymal
cell
(MSC)
applications
in
regenerative
medicine.
However,
information
on
rat
DPSCs
during
long-term
passage,
which
could
lead
replicative
senescence,
is
limited.
In
this
study,
we
investigated
the
phenotypic
changes
after
3–26
passages
(3P–26P).
The
results
show
that
morphology
and
nuclear
size
increase
proportionally
with
passage
number.
phosphorylated
histone
H2A.X
(γ-H2A.X)
positive
(indicating
DNA
damage)
increased
significantly
earlier
than
4-Hydroxynonenal
(4-HNE)
stained
an
abundance
of
intracellular
reactive
oxygen
species).
Compared
subjected
3P
5P,
15P
showed
reduced
proliferation
despite
being
for
Ki67.
Furthermore,
growth
was
completely
arrested
26P.
senescence
markers,
senescence-associated
β-galactosidase
(SA-β-gal)
p16,
exhibited
similar
expression
patterns
were
not
correlated
those
p21
urokinase-type
plasminogen
activator
receptor
(uPAR).
Nearly
all
expressed
SA-β-gal
p16
26P,
whereas
only
half
uPAR.
These
will
contribute
understanding
characteristics
toward
are
applicable
mechanisms
related
medicine
aging.
