Expanding the Potential of Circular RNA (CircRNA) Vaccines: A Promising Therapeutic Approach
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(1), С. 379 - 379
Опубликована: Янв. 4, 2025
In
recent
years,
circular
RNAs
(circRNAs)
have
garnered
significant
attention
due
to
their
unique
structure
and
function,
positioning
them
as
promising
candidates
for
next-generation
vaccines.
The
circRNA
vaccine,
an
RNA
offers
advantages
in
preventing
infectious
diseases
by
serving
a
vector
protein
expression
through
non-canonical
translation.
Notably,
vaccines
demonstrated
enduring
antigenic
generate
larger
percentage
of
neutralizing
antibodies
compared
mRNA
administered
at
the
same
dosage.
Furthermore,
can
elicit
robust
cellular
humoral
immunity,
indicating
potential
tumor
vaccine
development.
However,
certain
challenges
must
be
addressed
facilitate
widespread
use
both
disease
prevention
treatment.
These
include
low
efficiency
linear
circularization,
suboptimal
targeting
delivery
systems,
assessment
side
effects.
This
work
aims
describe
characteristics
functions
circRNAs,
elucidate
mechanism
behind
vaccines,
discuss
applications
treatment
tumors,
along
with
future
applications.
Язык: Английский
LncRNAs in serum-derived extracellular vesicles are potential biomarker and correlated with immune infiltration in gastric cancer
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 24, 2025
Background
Long
non-coding
RNAs
(lncRNAs)
in
extracellular
vesicles
(EVs)
have
been
confirmed
as
effective
non-invasive
biomarkers
for
multiple
diseases.
However,
their
expression
and
clinical
value
gastric
cancer
(GC)
remain
poorly
understood.
Materials
methods
Serum
EV
RNA
was
extracted
from
four
patients
with
GC
healthy
controls,
followed
by
high-throughput
sequencing.
LncRNAs
were
further
validated
training
validation
sets
using
quantitative
real-time
reverse
transcription
polymerase
chain
reaction.
Results
A
total
of
37,684
lncRNAs
obtained,
10
selected
based
on
the
criteria
(
P
<
0.05
|log
2
FoldChange|
≥1).
lncRNA
RMRP,
RPPH1,
linc-ROR
significantly
higher
than
those
chronic
gastritis,
atypical
hyperplasia,
or
control
(all
0.05).
Three
also
correlated
tumor
diameter,
lymphatic
metastasis,
distal
TNM
stage
The
area
under
curve
(AUC)
values
0.727,
0.774,
0.811,
respectively.
Corresponding
sensitivity
specificity
63.4%
85.4%,
50.7%
89.6%,
78.5%
66.7%.
combination
these
three
carcinoembryonic
antigen
(CEA)
yielded
an
AUC
0.909,
a
83.3%
each.
Furthermore,
high
RMRP
levels
associated
worse
disease-free
survival
overall
(OS).
Univariate
multivariate
Cox
regression
analyses
that
only
independent
prognostic
factor
GC.
Finally,
lncRNA-miRNA-mRNA
network
showed
predicted
to
interact
15
miRNAs
69
mRNAs.
In
addition,
immune
cell
infiltration,
including
neutrophils,
central
memory
CD4
T
cells,
macrophage,
natural
kill
cells.
Conclusion
are
prospective
biomarker
infiltration
It
provides
foundation
development
serum
EV-targeted
novel
immunotherapy
targets
Язык: Английский
Long Non-Coding RNAs in Malignant Human Brain Tumors: Driving Forces Behind Progression and Therapy
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(2), С. 694 - 694
Опубликована: Янв. 15, 2025
Long
non-coding
RNAs
(lncRNAs)
play
a
pivotal
role
in
regulating
gene
expression
and
are
critically
involved
the
progression
of
malignant
brain
tumors,
including
glioblastoma,
medulloblastoma,
meningioma.
These
lncRNAs
interact
with
microRNAs
(miRNAs),
proteins,
DNA,
influencing
key
processes
such
as
cell
proliferation,
migration,
invasion.
This
review
highlights
multifaceted
impact
lncRNA
dysregulation
on
tumor
underscores
their
potential
therapeutic
targets
to
enhance
efficacy
chemotherapy,
radiotherapy,
immunotherapy.
The
insights
provided
offer
new
directions
for
advancing
basic
research
clinical
applications
tumors.
Язык: Английский
TFAP2A-regulated CRNDE enhances colon cancer progression and chemoresistance via RIPK3 interaction
Functional & Integrative Genomics,
Год журнала:
2025,
Номер
25(1)
Опубликована: Фев. 15, 2025
Colon
cancer
(CC)
is
a
common
malignancy
with
rising
incidence
worldwide.
Despite
advances
in
treatment
strategies,
many
patients
still
face
poor
prognosis
due
to
the
development
of
drug
resistance.
Long
non-coding
RNAs
(lncRNAs)
have
emerged
as
important
regulators
various
biological
processes
and
been
implicated
progression.
Among
them,
colorectal
neoplasia
differentially
expressed
(CRNDE)
has
drawn
attention
for
its
potential
roles
different
cancers.
However,
specific
functions
CC
remain
unclear.
In
this
study,
we
identified
CRNDE
highly
CC,
contributing
tumor
progression
Mechanically,
regulated
by
transcription
factor
TFAP2A.
Additionally,
inhibits
pyroptosis,
form
programmed
cell
death,
promoting
ubiquitin-mediated
degradation
RIPK3,
thereby
reducing
sensitivity
cells
5-fluorouracil
(5-FU).
Our
findings
suggest
that
TFAP2A/CRNDE/RIPK3
axis
plays
critical
colon
chemoresistance,
highlighting
therapeutic
targets
improving
outcomes.
Язык: Английский
Investigating potential drug targets for the treatment of glioblastoma: a Mendelian randomization study
BMC Cancer,
Год журнала:
2025,
Номер
25(1)
Опубликована: Апрель 10, 2025
Glioblastoma
(GBM),
one
of
the
most
aggressive
brain
tumors,
has
a
5-year
survival
rate
less
than
5%.
Current
standard
therapies,
including
surgery,
radiotherapy,
and
temozolomide
(TMZ)
chemotherapy,
are
limited
by
drug
resistance
blood-brain
barrier.
Integrating
expression
quantitative
trait
loci
(eQTL)
protein
(pQTL)
data
shown
promise
in
uncovering
disease
mechanisms
therapeutic
targets.
This
study
combined
eQTL
pQTL
analyses
to
identify
potential
GBM-related
genes
circulating
plasma
proteins
for
exploration.
Using
transcriptomic
from
The
Cancer
Genome
Atlas
(TCGA),
we
identified
2,528
differentially
expressed
genes,
GPX7
CXCL10.
eQTL-MR
analysis
identifies
GBM-associated
constructs
protein-protein
interaction
(PPI)
network.Integrating
deCODE
database,
pQTL-MR,
colocalization
validated
CXCL10.These
findings
provide
new
perspectives
on
GBM
biology
suggest
actionable
targets
therapy.
Despite
limitations
due
sample
size
population-specific
data,
this
highlights
CXCL10
as
promising
candidates
further
investigation
lays
foundation
targeted
treatments.
Язык: Английский
Targeting the dual miRNA/BMP2 network: LncRNA H19-mediated temozolomide resistance unveils novel therapeutic strategies in glioblastoma
Frontiers in Oncology,
Год журнала:
2025,
Номер
15
Опубликована: Апрель 14, 2025
Background
Long
noncoding
RNA
(lncRNA)
is
known
to
not
only
be
involved
in
various
biological
processes
but
also
play
a
crucial
role
chemotherapy
resistance.
The
development
of
resistance
glioblastoma
(GBM)
poses
significant
challenge
clinical
settings.
Nonetheless,
the
mechanisms
through
which
lncRNA
contributes
acquired
Temozolomide
(TMZ)
GBM
patients
remain
unclear.
Methods
We
identified
265
upregulated
and
396
downregulated
lncRNAs
associated
with
chemoresistance
from
GEO
database
(GSE100736).
Subsequently,
we
assessed
expression
levels
H19,
hsa-miR-138-5p,
hsa-miR-22-3p,
BMP2
mRNA
quantitative
polymerase
chain
reaction
(qPCR)
cells
TMZ-resistant
cells.
Cell
viability
proliferation
were
evaluated
using
CCK-8
cell
colony
formation
assays,
respectively.
Apoptosis
was
determined
flow
cytometry
analysis.
impact
gene
overexpression
knockdown
on
apoptosis
examined
via
transfection
experiments.
Furthermore,
investigated
influence
H19
tumor
an
vivo
xenograft
model.
Results
upregulation
observed
lines
tissues,
suggesting
its
involvement
TMZ
Silencing
restored
sensitivity
resistant
vitro
.
Conversely,
promoted
hindered
TMZ-triggered
apoptosis,
facilitating
acquisition
Notably,
functions
as
molecular
decoy
for
hsa-miR-138-5p
these
miRNAs
can
reverse
induced
by
Additionally,
H19-mediated
pathway
Knockdown
reinstated
,
whereas
Conclusion
LncRNA
enhances
competitive
targeting
BMP2.
Язык: Английский