Advancements in Microfluidic Technologies for Male Infertility
Advanced Materials Technologies,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 7, 2025
Abstract
Infertility
affects
≈15%
of
couples
worldwide,
with
≈45%
these
cases
involving
male
factors.
Semen
analysis
and
sperm
selection
are
critical
routine
steps
in
achieving
successful
assisted
reproductive
outcomes.
Conventional
methods,
which
widely
used
clinics,
manual,
subjective,
time‐consuming,
simply
not
sufficient
for
the
highly
complex
multifaceted
task
analysis.
Recently,
microfluidics‐based
devices,
combined
high‐resolution
microscopy,
have
offered
promising
opportunities
evaluating
quality,
gaining
a
fundamental
understanding
motion,
high‐quality
sperm.
Machine
learning
(ML)
has
also
introduced
automation
standardization
analyzing
morphology,
intracellular
characteristics,
motility.
In
this
review,
state‐of‐the‐art
methods
comprehensively
discussed
provide
directions
to
address
unresolved
challenges.
Язык: Английский
Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges
Lenka Hackerová,
Aneta Pilsova,
Zuzana Pilsova
и другие.
Animals,
Год журнала:
2025,
Номер
15(3), С. 305 - 305
Опубликована: Янв. 22, 2025
Spermatozoa
are
highly
specialized
male
cells
that
characterized
by
a
unique
ability
to
move,
which
is
critical
factor
for
successful
fertilization.
The
relative
simplicity
of
motility
assessment,
especially
in
livestock,
has
made
it
widely
used
parameter
evaluating
ejaculate
quality
or
cryopreserved
semen
the
clinical
field,
and
an
advanced
tool
reproductive
physiology
toxicology
research.
Technological
advances
image
analysis
computational
methods
have
substantially
increased
its
accuracy
through
use
computer-assisted
sperm
(CASA)
minimize
subjective
bias
assessments.
Nevertheless,
this
more
objective
method
still
presents
some
significant
challenges,
including
variability
sample
preparation,
imaging
conditions,
analytical
parameters.
These
issues
contribute
inconsistency
impair
reproducibility
comparability
data
between
laboratories.
implementation
standardized
protocols,
combined
with
comprehensive
training
rigorous
evaluation,
can
serve
mitigate
emerging
inconsistencies.
In
addition,
vitro
conditions
under
CASA
analyses
performed
often
differ
significantly
from
natural
environment
female
tract
vivo.
This
review
discusses
methodologies,
issues,
limitations
using
CASA,
particular
focus
on
boar
as
important
agricultural
biomedical
model
species
system
used.
Язык: Английский
A Neuromorphic Camera for Tracking Passive and Active Matter with Lower Data Throughput
Laser & Photonics Review,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 29, 2025
Abstract
The
merits
of
using
a
neuromorphic,
or
event‐based
camera
(EBC),
for
tracking
both
passive
and
active
matter
are
demonstrated.
For
matter,
the
Brownian
motion
different
micro‐particles
is
tracked
their
diffusion
coefficients
estimated.
case
murine
spermatozoa
explored
motility
parameters
extracted
from
cells.
This
has
applications
in
enhancing
outcomes
clinical
fertility
treatments.
Using
EBC,
results
equivalent
to
those
an
sCMOS
obtained,
while
achieving
reduction
file
size
up
two
orders
magnitude.
important
modern
computer
era,
as
it
reduces
data
throughput,
well‐aligned
with
edge‐computing
applications.
EBC
considered
excellent
choice,
particularly
long‐term
studies
matter.
Язык: Английский
A novel CCDC112-dependent process of sperm midpiece formation and epididymal maturation
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 5, 2024
Abstract
The
sperm
mitochondrial
sheath
has
proposed
functions
in
structural
support
and
energy
production
for
motility.
Here
we
define
coiled
coil
domain
containing
protein
112,
CCDC112,
as
crucial
male
fertility,
specifically
the
assembly
function
of
sheath.
We
unveiled
a
previously
unrecognised
process
epididymal
maturation.
Sperm
sheaths
are
structurally
immature
upon
exiting
testis
continue
to
mature
they
transit
from
caput
cauda
epididymis.
Data
reveal
critical
role
CCDC112
morphogenesis
during
formation.
A
lack
lead
significantly
reduced
respiration
capacity,
irregular
flagellar
waveforms,
diminished
progressive
motility
failure
traverse
female
reproductive
tract
site
fertilisation
penetrate
zonae
pellucidae
oocytes.
Finally,
identify
component
distal
appendages
mother
centriole
facilitative
core
tail
assembly.
Collectively,
is
key
regulator
while
further
expanding
understanding
spermatogenesis,
generation
flagella
kinematics.
Язык: Английский