Development of the semicircular canals and otolithic organs of the vertebrate inner ear

Current topics in developmental biology/Current Topics in Developmental Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Identification and engineering of the aprE regulatory region and relevant regulatory proteins in Bacillus licheniformis 2709

Enzyme and Microbial Technology, Год журнала: 2023, Номер 172, С. 110310 - 110310

Опубликована: Авг. 28, 2023

Язык: Английский

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Kremen1 regulates the regenerative capacity of support cells and mechanosensory hair cells in the zebrafish lateral line

iScience, Год журнала: 2023, Номер 27(1), С. 108678 - 108678

Опубликована: Дек. 7, 2023

Mechanosensory hair cells in the inner ear mediate sensations of hearing and balance, specialized lateral line sensory system aquatic vertebrates, sensation water movement. In mammals, lack ability to regenerate following damage, resulting deficits. contrast, non-mammalian such as zebrafish, can renew throughout their lifespan. Wnt signaling is required for development regulates regeneration. Kremen1 inhibits cell formation, though its role regeneration unknown. We used a zebrafish

Язык: Английский

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Transdifferentiation is uncoupled from progenitor pool expansion during hair cell regeneration in the zebrafish inner ear

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 11, 2024

Death of mechanosensory hair cells in the inner ear is a common cause auditory and vestibular impairment mammals, which have limited ability to regrow these after damage. In contrast, non-mammalian vertebrates including zebrafish can robustly regenerate following severe organ The provides an understudied model system for understanding cell regeneration organs that are highly conserved with their mammalian counterparts. Here we quantitatively examine addition during growth larval ear. We used genetically encoded ablation method induce death observed gradual correct spatial patterning over two weeks ablation. Supporting cells, surround source new divide response ablation, expanding possible progenitor pool. parallel, nascent arise from direct transdifferentiation pool uncoupled division. These findings reveal previously unrecognized mechanism implications how may be encouraged

Язык: Английский

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Zebrafish regulatory genomic resources for disease modelling and regeneration

Disease Models & Mechanisms, Год журнала: 2023, Номер 16(8)

Опубликована: Авг. 1, 2023

In the past decades, zebrafish has become a disease model with increasing popularity owing to its advantages that include fast development, easy genetic manipulation, simplicity for imaging, and sharing conserved disease-associated genes pathways those of human. parallel, studies mechanisms are increasingly focusing on non-coding mutations, which require genome annotation maps regulatory elements, such as enhancers promoters. line this, genomic resources research expanding, producing variety data help in defining elements their conservation between humans. Here, we discuss recent developments generating functional how this can be applied human diseases. We highlight community-driven developments, DANIO-CODE, centralised standardised catalogue genomics annotations; consider limitations current maps; offer considerations interpreting integrating existing comparative tools. also need developing protocols bioinformatic pipelines provide suggestions development analysis visualisation tools will integrate various multiomic bulk sequencing together fast-expanding single-cell methods, assay transposase-accessible chromatin sequencing. Such integration essential exploit characterisation offered by cell-type resolution emerging methods. Together, these advances build an expansive toolkit interrogating zebrafish.

Язык: Английский

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Advance and Application of Single-cell Transcriptomics in Auditory Research

Neuroscience Bulletin, Год журнала: 2023, Номер 40(7), С. 963 - 980

Опубликована: Ноя. 28, 2023

Hearing loss and deafness, as a worldwide disability disease, have been troubling human beings. However, the auditory organ of inner ear is highly heterogeneous has very limited number cells, which are largely uncharacterized in depth. Recently, with development utilization single-cell RNA sequencing (scRNA-seq), researchers able to unveil complex sophisticated biological mechanisms various types cells at level address challenges cellular heterogeneity that not resolved through by conventional bulk (bulk RNA-seq). Herein, we reviewed application scRNA-seq technology research, aim providing reference for organs, pathogenesis hearing loss, regenerative therapy. Prospects about spatial transcriptomic scRNA-seq, based genome, Live-seq will also be discussed.

Язык: Английский

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Single-Cell Transcriptomic Profiling of the Zebrafish Inner Ear Reveals Molecularly Distinct Hair Cell and Supporting Cell Subtypes

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Сен. 10, 2022

Abstract A major cause of human deafness and vestibular dysfunction is permanent loss the mechanosensory hair cells inner ear. In non-mammalian vertebrates such as zebrafish, regeneration missing can occur throughout life. While a comparative approach has potential to reveal basis differential regenerative ability, degree which ears fish mammals share common supporting cell types remains unresolved. Here we perform single-cell RNA sequencing zebrafish ear at embryonic through adult stages catalog diversity non-sensory cells. We identify putative progenitor population for cells, well distinct in maculae versus cristae. The differ from those described lateral line system, distributed organ most studies have been conducted. maculae, two subtypes that gene expression with mammalian striolar or extrastriolar situ hybridization reveals these occupy spatial domains within macular organs, utricle saccule, consistent reported electrophysiological properties domains. These findings suggest primitive specialization spatially likely arose last ancestor mammals. similarities type composition between also support using relevant model understanding ear-specific function regeneration.

Язык: Английский

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Advanced Omics Techniques for Understanding Cochlear Genome, Epigenome, and Transcriptome in Health and Disease

Biomolecules, Год журнала: 2023, Номер 13(10), С. 1534 - 1534

Опубликована: Окт. 17, 2023

Advanced genomics, transcriptomics, and epigenomics techniques are providing unprecedented insights into the understanding of molecular underpinnings central nervous system, including neuro-sensory cochlea inner ear. Here, we report for first time a comprehensive updated overview most advanced omics study nucleic acids their applications in cochlear research. We describe available vitro vivo models hearing research principles epigenomics, alongside technologies (like single-cell spatial omics), which allow investigation events that occur at resolution while retaining information.

Язык: Английский

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Embracing the diversity of model systems to deconstruct the basis of regeneration and tissue repair

Development, Год журнала: 2023, Номер 150(3)

Опубликована: Янв. 31, 2023

ABSTRACT The eighth EMBO conference in the series ‘The Molecular and Cellular Basis of Regeneration Tissue Repair’ took place Barcelona (Spain) September 2022. A total 173 researchers from across globe shared their latest advances deciphering molecular cellular basis wound healing, tissue repair regeneration, as well implications for future clinical applications. showcased an ever-expanding diversity model organisms used to identify mechanisms that promote regeneration. Over 25 species were discussed, ranging invertebrates humans. Here, we provide overview exciting topics presented at conference, highlighting novel discoveries regeneration perspectives regenerative medicine.

Язык: Английский

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In preprints: theme and variations on hair-cell regeneration in zebrafish

Development, Год журнала: 2024, Номер 151(14)

Опубликована: Июль 15, 2024

Imagine reaching old age and still being able to engage effortlessly in conversations (Cheslock De Jesus, 2023). This possibility hinges on preserving the mechanosensory hair cells we are born with, which mammals exceptionally long-lived but, with very few exceptions, cannot be replaced once lost (Kirkegaard Jørgensen, 2000; Savas, In humans, loss of these results chronic hearing deficits, making everyday activities such as conversing noisy settings or talking phone overwhelming challenges (Reynard Thai-Van, 2024). For many, this impairment not only isolates them from social interactions but also aggravates mental health issues anxiety depression (Luppa et al., Most insights into maintaining function come studying non-mammalian vertebrates, can regenerate naturally throughout their lives (Choi 2024; Denans 2019; Pinto-Teixeira 2013; Benkafadar Emerging research is revealing that multiple mechanisms regulate hair-cell regeneration, even within same species, offering hope for future breakthroughs treating humans.Among most studied hair-cell-bearing organs neuromasts lateral line, enable fishes amphibians detect low-frequency mechanical fluctuations surrounding water (Valera 2021; Tidswell These aquatic vertebrates use line orient relative flow direction, escape predators locate prey, develop avoidance reactions. The take a more prominent role when vision limited (Montgomery 2000). Fishes have ears, constituent control balance high-frequency signals (Liu Bagnall, Importantly, ears must remain functional life animal, despite persistent environmental insult cells. zebrafish has always offered researchers two key advantages (Barrallo-Gimeno Llorens, 2022; Plazas Elgoyhen, Holmgren Sheets, Pickett Raible, 2019). First, unparalleled accessibility high-resolution microscopy, chiefly thanks its superficial location availability many lines expressing various genetically encoded fluorescent makers label different cell types (Pinto-Teixeira 2015; Hewitt Second, quickly easily eliminated using pharmacological, genetic physical approaches. ensuing recovery occurs days, enabling high temporal-resolution recording entire regenerative process 2013). recent years, advances single-cell transcriptomics added above (Lush Baek Kozak 2020). Single-cell RNA sequencing (scRNA-seq) hairpin chain reaction-based situ hybridization (HCR-FISH) helped molecularly characterize cellular sub-populations (Shi Moreover, easy production mutant animals gives access molecular components drive regeneration (Parvez 2024).The initial discovery resident progenitors took place almost decades ago (López-Schier Hudspeth, 2006). Every study followed agreed general mechanism follows series steps start birth new pairs mitotic division unipotent (UHCP; Thomas Mackenzie 2012). Although it been established how supporting re-enter mitosis after death, direct UHCP behavior remained obscure (Kozak preprint, Bell colleagues evidence scRNA-seq experiments showing gene foxg1a expressed during (Bell 2024 preprint). They allele find reduced number neuromast formation. Using nls-Eos transgenic photoconvertible protein disentangle effect fox1ga mutants neuromast, they conclude Foxg1a controls proliferation population isl1a-expressing central progenitor cells.However, nagging issue field found ear zebrafish, readily (Jimenez 2022). There currently no known marker specific UHCP, unambiguous identification achieved live videomicroscopy Therefore, one explanation finding much difficult image live. Another described simply do exist ear. another Beaulieu show robust through markedly differs neuromasts, resembling closely birds (Beaulieu preprint; Bhave 1995). Here, ablation, arise transdifferentiation pool Transdifferentiation identity conversion without an immediate intervening (Wang traditionally term reserved interconversion between belonging lineages, agree authors preprint sensu lato would entail any postmitotic another. transient wave precursor otherwise depleted by continuous maintain stable preprint) used data HCR-FISH distinguish location: cabp1b-positive recently differentiated peripheral, whereas scn5lab-expressing located centrally ear's crista. A clever combination capsaicin-mediated ablation system allowed measure possible alternative interpretation inner produces pairs, does, progenitor. Then, identically birds, immediately takes mature identity, sibling remains hidden dormant immature state until further death induces emergence (Stone Rubel, Regardless, interesting what mammalian and, therefore, may clinically relevant humans.Together, preprints highlight need combine studies understand regeneration. will benefit taking advantage scRNA-seq-derived data, together newly-developed phiC31 Integrase Genomic Loci Engineered Transgenesis (pIGLET) generate diverse reproducible expression patterns compare populations behave (Lalonde Brown 2023).Beaulieu al. (2024 now accepted as: Beaulieu, M. O., Thomas, E. D. W. (2024). temporally uncoupled expansion Development 151, dev202944. doi:10.1242/dev.202944.

Язык: Английский

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