Novel Research in Microbiology Journal,
Год журнала:
2023,
Номер
7(6), С. 2248 - 2264
Опубликована: Ноя. 14, 2023
Biofilm
formation
by
Escherichia
coli
presents
a
major
challenge
in
the
clinical
settings,
resulting
persistent
infections
and
treatment
failures.
These
bacterial
communities,
protected
matrix,
resist
antibiotics
immune
responses,
thus
causing
prolonged
to
treat
such
infections.
Developing
effective
strategies
against
E.
biofilms
is
crucial
for
improving
patient
outcomes
reducing
burden
on
healthcare
systems.
This
study
aimed
extract,
purify,
characterize
mannan
from
Saccharomyces
cerevisiae,
then
its
antibiofilm
activity
was
evaluated
multi-drug
resistant
(MDR-E.
coli)
isolates
obtained
various
sources
(i.e.,
urine,
stool,
wound,
catheter).
Using
standardized
protocol
with
slight
modifications,
crude
extraction
yielded
37.6
%,
subsequent
purification
achieved
an
efficiency
of
99.6
%.
Characterization
assays
purified
included
FT-IR;
FE-SEM,
carbohydrate
content
estimation,
solubility,
melting
point
tests,
which
revealed
presence
α-1,6
α-1,2
linked
sugars;
crystalline
nature,
high
porosity
(80
%)
content,
solubility
water,
248
°C.
The
exhibited
substantial
ability
inhibit
biofilm
(37.50
degrade
existing
MDR-E.
(37.43
%).
findings
underscore
potential
S.
cerevisiae
as
agent
applications.
Further
exploration
optimization
mannan's
therapeutic
are
essential
fully
leverage
efficacy
combating
biofilm-associated
caused
coli.
Crystal Growth & Design,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
The
preparation
of
salts
diclofenac
(DCF)
with
alkyl
amines
is
an
important
pharmaceutical
formulation
in
terms
improving
its
skin
permeability.
However,
no
trend
permeability
changes
has
been
reported
the
case
primary
ammonium
DCF.
Herein,
we
have
prepared
four
DCF
carbon
chain
length,
that
is,
n-butylamine
(nBA),
isoamylamine
(isoAA),
n-hexylamine
(nHA),
and
2-phenylethylamine
(phEA).
Salts
were
by
dissolving
acetonitrile,
followed
stoichiometric
addition
amines.
Crystals
grown
from
acetonitrile–methanol
mixture
solvents
using
slow
evaporation
technique
under
ambient
conditions.
Structural
characterization
salt
forms
DCF–·nBA+,
DCF–·isoAA+,
DCF–·nHA+,
DCF–·phEA+
was
carried
out
single-crystal
X-ray
diffraction.
All
crystallized
anhydrate
form
are
phase
stable
Differential
scanning
calorimetry
thermograms
showed
had
good
thermal
stability
except
which
a
solid–solid
transition
at
∼114
°C
ΔH
≈
12
J/g.
lower
melting
endotherm
than
free
acid.
thermodynamic
analyzed
slurry
method
acidic
pH
1.2
7
aqueous
buffer
solution.
disproportionated
to
acid
but
Solubility,
dissolution,
experiments
performed
flux
through
semipermeable
membrane
highest
for
DCF–·nBA+
lowest
There
solubility
dissolution
amine
observed
lipophilicity
amine.
It
interesting
note
relation
between
molecular
volume/molecular
weight
trend.
Due
tight
ion-pairing
counterions
carboxylate,
behave
as
neutral
species
during
permeation,
rate
transport
determined
mobility
salt.
Therefore,
inverse
their
hence
bioavailability.
In
previous
studies,
such
correlation
reported.
This
study
establishes
ion
pairs
Among
forms,
bioavailability
∼1000
times
higher
International Journal of Pharmaceutics,
Год журнала:
2024,
Номер
654, С. 123977 - 123977
Опубликована: Март 6, 2024
Bottom-up
production
of
active
pharmaceutical
ingredient
(API)
crystal
suspensions
offers
advantages
in
surface
property
control
and
operational
ease
over
top-down
methods.
However,
downstream
separation
concentration
pose
challenges.
This
proof-of-concept
study
explores
membrane
diafiltration
as
a
comprehensive
solution
for
processing
API
produced
via
anti-solvent
crystallization.
It
involves
switching
the
residual
solvent
(N-methyl-2-pyrrolidone,
NMP)
with
water,
adjusting
excipient
(d-α-Tocopherol
polyethylene
glycol
1000
succinate,
TPGS)
quantity,
enhancing
loading
(solid
concentration)
itraconazole
suspensions.
NMP
was
decreased
from
9
wt%
to
below
0.05
(in
compliance
European
Medicine
Agency
guidelines),
while
TPGS
0.475
0.07
wt%.
reduced
TPGS-to-itraconazole
ratio
1:2
less
than
1:50
raised
1
35.6
Importantly,
these
changes
did
not
adversely
affect
stability
suspension.
presents
one-step
address
challenges
bottom-up
suspension
production.
These
findings
contribute
optimizing
manufacturing
processes
hold
promise
advancing
development
long-acting
techniques
at
commercial
scale.
Pharmaceutics,
Год журнала:
2024,
Номер
16(8), С. 1071 - 1071
Опубликована: Авг. 15, 2024
This
research
work
dives
into
the
complexity
of
hot-melt
extrusion
(HME)
and
its
influence
on
drug
stability,
focusing
solid
dispersions
containing
30%
glibenclamide
three
50:50
polymer
blends.
The
polymers
used
in
study
are
Ethocel
Standard
10
Premium,
Kollidon
SR
Affinisol
HPMC
HME
4M.
Glibenclamide
characterized
using
thermal
analyses
(thermogravimetric
analysis
(TGA)
differential
scanning
calorimetry),
X-ray
diffraction
electron
microscopy.
reveals
transformation
impurity
A
during
process
mass
spectrometry
TGA.
Thus,
it
enables
quantification
extent
degradation.
Furthermore,
this
shows
how
polymer–polymer
blend
matrices
exert
an
impact
parameters,
active
pharmaceutical
ingredient’s
physical
state,
release
behavior.
In
vitro
dissolution
studies
show
that
polymeric
investigated
provide
extended
(over
24
h),
mainly
dictated
by
polymer’s
chemical
nature.
paper
highlights
is
degraded
selection
crucially
affects
sustained
dynamics.
Pharmaceutics,
Год журнала:
2024,
Номер
16(12), С. 1488 - 1488
Опубликована: Ноя. 21, 2024
Background:
Supersaturating
drug
delivery
systems
(SDDSs)
have
gained
significant
attention
as
a
promising
strategy
to
enhance
the
solubility
and
bioabsorption
of
Biopharmaceutics
Classification
System
(BCS)
II
drugs.
To
overcome
challenges
associated
with
polymer-based
amorphous
SDDS
(aSDDS),
coamorphous
(CAM)
emerged
viable
alternative.
Among
them,
“drug-drug”
CAM
(ddCAM)
show
considerable
potential
for
combination
therapy.
However,
many
drugs
in
their
pure
forms
are
unstable
at
room
temperature
(RT),
complicating
formation
long-term
stability
profiles.
Consequently,
limited
knowledge
exists
regarding
behavior
ddCAMs
containing
RT-unstable
components
formed
via
quench
cooling.
Methods:
In
this
study,
we
used
naproxen
(NAP),
drug,
felodipine
(FEL)
or
nitrendipine
(NTP),
two
RT-stable
drugs,
create
“FEL-NAP”
“NTP-NAP”
ddCAM
pairs
Our
work
series
methods
perform
detailed
analysis
on
co-amorphization,
dissolution,
solubility,
profiles
contributing
advancements
co-amorphization
techniques
generating
SDDS.
Results:
This
study
revealed
that
produced
quench-cooling
method
were
closely
related
drug-drug
pairing
types
ratios.
Both
incorporation
into
improved
physical
individual
APIs.
Conclusions:
findings
provide
deeper
insight
characteristics
specific
FEL-NAP
NTP-NAP,
offering
valuable
guidance
developing
new
formulations
some
Journal of Pharmaceutical Sciences,
Год журнала:
2023,
Номер
112(9), С. 2463 - 2482
Опубликована: Апрель 7, 2023
Ball-milling
and
harsh
manufacturing
processes
often
generate
crystal
disorder
which
have
practical
implications
on
the
physical
chemical
stabilities
of
solid
drugs
during
subsequent
storage,
transport,
handling.
The
impact
state
drugs,
containing
different
degrees/levels
disorder,
their
autoxidative
stability
under
storage
has
not
been
widely
investigated.
This
study
investigates
differing
degrees
autoxidation
Mifepristone
(MFP)
to
develop
a
predictive
(semi-empirical)
model.
Crystalline
MFP
was
subjected
durations
ambient
ball
milling,
resulting
disorder/
amorphous
content
quantified
using
partial
least
square
(PLS)
regression
model
based
Raman
spectroscopy
data.
Samples
milled
varying
levels
were
range
(accelerated)
conditions,
periodically
sampled
examine
recrystallization
degradation
extents.
Crystallinity
monitored
by
spectroscopy,
evaluated
liquid
chromatography.
analyses
samples
demonstrated
competition
between
via
MFP,
extents
depending
conditions/exposure
time.
kinetics
analyzed
accounting
for
preceding
content,
fitted
with
diffusion
An
extended
Arrhenius
equation
used
predict
stored
long-term
(25°C/60%
RH)
accelerated
(40°C/75%
RH,
50°C/75%
conditions.
highlights
utility
such
identifying
instability
in
non-crystalline/partially
crystalline
owing
phases.
is
particularly
useful
drug-product
leveraging
concept
material
sciences.
