A PPR Protein RFCD1 Affects Chloroplast Gene Expression and Chloroplast Development in Arabidopsis

Plants, Год журнала: 2025, Номер 14(6), С. 921 - 921

Опубликована: Март 15, 2025

Chloroplast development is a highly complex process, involving many regulatory mechanisms that remain poorly understood. This study reports novel PPR protein, RFCD1 (Regulation Factor of Development 1). Fluorescence localization analysis reveals the N-terminal 60 amino acids fused with GFP protein specifically direct to chloroplast. The knockout mutant embryo-lethal. RNA interference (RNAi) transgenic lines display chlorosis phenotypes and abnormal chloroplast development. Quantitative real-time PCR (qRT-PCR) showed expression levels plastid-encoded polymerase (PEP) genes were significantly decreased in RNAi lines. Furthermore, blotting results RNA-seq data processing plastid rRNA was also affected Taken together, these indicate might be involved gene processing, which essential for Arabidopsis.

Язык: Английский

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Regreening mechanisms in cucumber: insights from a CsSIG2 mutation affecting chloroplast development

Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)

Опубликована: Март 23, 2025

Язык: Английский

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GhCTSF1, a short PPR protein with a conserved role in chloroplast development and photosynthesis, participates in intron splicing of rpoC1 and ycf3-2 transcripts in cotton

Plant Communications, Год журнала: 2024, Номер 5(6), С. 100858 - 100858

Опубликована: Март 5, 2024

Cotton is one of the most important textile fibers worldwide. As crucial agronomic traits, leaves play an essential role in growth cotton plants, disease resistance, fiber quality, and yield. Pentatricopeptide repeat (PPR) proteins are a large family nuclear-encoded involved organellar or nuclear RNA metabolism. Using virus-induced gene silencing (VIGS) assay, we found that plants displayed variegated yellow leaf phenotypes with decreased chlorophyll content when expression PPR named GhCTSF1. GhCTSF1 encodes chloroplast-localized protein containing only two motifs. Disruption substantially reduced splicing efficiency rpoC1 intron 1 ycf3 2. Loss function ortholog EMB1417 causes defects ycf3-2, leading to impaired chloroplast structure photosynthetic rates Arabidopsis. Moreover, interacted factors, GhCRS2 GhWTF1. Defects GhWTF1 severely affect ycf3-2 cotton, development reduction photosynthesis. Our results suggest specifically required for cooperation

Язык: Английский

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Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli

Archives of Microbiology, Год журнала: 2024, Номер 206(4)

Опубликована: Март 12, 2024

Язык: Английский

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Chloroplast biogenesis involves spatial coordination of nuclear and organellar gene expression in Chlamydomonas

PLANT PHYSIOLOGY, Год журнала: 2024, Номер 196(1), С. 112 - 123

Опубликована: Май 6, 2024

Abstract The localization of translation can direct the polypeptide product to proper intracellular compartment. Our results reveal by cytosolic ribosomes on a domain chloroplast envelope in unicellular green alga Chlamydomonas (Chlamydomonas reinhardtii). We show that this isolated chloroplasts retains translationally active and mRNAs encoding proteins. This is aligned with localized zone, compartment where photosystem subunits encoded plastid genome are synthesized assembled. Roles directing newly photosynthesis complexes discrete regions within for their assembly suggested differences either subunit light-harvesting complex II or small Rubisco. Transcription spatially coordinated translation, as revealed our demonstration subpopulation transcriptionally nucleoids at zone. propose expression proteins nuclear-cytosolic organellar genetic systems organized subcompartments cytoplasm facilitate biogenesis photosynthetic complexes.

Язык: Английский

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Comparative and phylogenetic analysis of the chloroplast genomes of four commonly used medicinal cultivars of Chrysanthemums morifolium

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Окт. 22, 2024

Язык: Английский

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Architecture of the spinach plastid-encoded RNA polymerase

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 13, 2024

The plastid-encoded RNA polymerase serves as the principal transcription machinery within chloroplasts, transcribing over 80% of all primary plastid transcripts. This consists a prokaryotic-like core enzyme known core, and is supplemented by newly evolved associated proteins PAPs. However, architecture possible functions PAPs remain unknown. Here, we present cryo-electron microscopy structure 19-subunit complex derived from spinach (Spinacia oleracea). shows that resembles bacterial polymerase. Twelve two additional (FLN2 pTAC18) bind at periphery forming extensive interactions may facilitate assembly stability. also protect against oxidative damage has potential in transcriptional regulation. research offers structural basis for future investigations into regulatory mechanisms governing genes. chloroplasts. authors spinach.

Язык: Английский

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Arabidopsis GENOMES UNCOUPLED PROTEIN1 binds to plastid RNAs and promotes their maturation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 8, 2024

Abstract Plastid biogenesis and the coordination of plastid nuclear genome expression through anterograde retrograde signaling are essential for plant development. GENOMES UNCOUPLED1 (GUN1) plays a central role in during early The putative function GUN1 has been extensively studied, but its molecular remains controversial. Here, we evaluate published transcriptome data generate our own from gun1 mutants grown under relevant conditions to show that editing splicing not GUN1-dependent signaling. Our study (post)-transcriptome seedlings with white pale cotyledons demonstrates deficiency significantly alters entire transcriptome. By combining this result PPR code-based prediction experimental validation by RNA immunoprecipitation experiments, several targets were identified, including 23S rRNA, tRNAs RNAs derived ycf1.2 ndhH - ndhA ndhI ndhG ndhE psaC ndhD gene cluster. absence rRNAs significant reduction almost all transcripts account cotyledon phenotype. identifies binding maturation as long-sought resolves long-standing controversies. We anticipate findings will serve basis subsequent studies investigating mechanism facilitate elucidation GUN1’s

Язык: Английский

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GENOMES UNCOUPLED PROTEIN1 binds to plastid RNAs and promotes their maturation

Plant Communications, Год журнала: 2024, Номер 5(12), С. 101069 - 101069

Опубликована: Авг. 23, 2024

Plastid biogenesis and the coordination of plastid nuclear genome expression through anterograde retrograde signaling are essential for plant development. GENOMES UNCOUPLED1 (GUN1) plays a central role in during early The putative function GUN1 has been extensively studied, but its molecular remains controversial. Here, we evaluate published transcriptome data generate our own from gun1 mutants grown under signaling-relevant conditions to show that editing splicing not relevant GUN1-dependent signaling. Our study (post)transcriptome seedlings with white pale cotyledons demonstrates deficiency significantly alters entire transcriptome. By combining this result pentatricopeptide repeat code-based prediction experimental validation by RNA immunoprecipitation experiments, identified several targets GUN1, including tRNAs RNAs derived ycf1.2, rpoC1, rpoC2 ndhH-ndhA-ndhI-ndhG-ndhE-psaC-ndhD gene cluster. absence rRNAs significant reduction almost all transcripts account cotyledon phenotype. provides evidence binding maturation as long-sought resolves long-standing controversies. We anticipate findings will serve basis subsequent studies on mechanisms help elucidate

Язык: Английский

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Disruption of the rice ALS1 localized in chloroplast causes seedling-lethal albino phenotype

Plant Science, Год журнала: 2023, Номер 338, С. 111925 - 111925

Опубликована: Ноя. 20, 2023

Язык: Английский

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