Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives

Frontiers in Genetics, Год журнала: 2024, Номер 15

Опубликована: Янв. 24, 2024

Powdery mildew is one of the most severe diseases affecting wheat yield and quality caused by Blumeria graminis f. sp. tritici ( Bgt ). Host resistance preferred strategy to prevent this disease. However, narrow genetic basis common has increased demand for diversified germplasm resources against powdery mildew. Wheat relatives, especially secondary gene pool wheat, are important donors in improvement because its abundant variation close kinship with wheat. In study, a series 137 including 53 Triticum monococcum L. (2n = 2x 14, AA), 6 T. urartu Thumanjan ex Gandilyan 9 timopheevii Zhuk. 4x 28, AAGG), 66 aestivum subsp . spelta 6x 42, AABBDD), 3 Aegilops speltoides SS) were systematically evaluated their composition Pm genes. Out (60.58%) accessions, 83 resistant isolate E09 at seedling stage, 116 (84.67%) relatives mixture isolates adult stage. This indicates that these accessions show high level Some 31 markers 23 known genes used test and, results, only Pm2 , Pm4 Pm6 Pm58 Pm68 detected. Among them, three alleles Pm4a Pm4b Pm4f ) identified 4 T accessions. q-RT PCR further confirmed played role disease four The phylogenetic tree showed was Pm24 Sr62 study not provides reference information valuable breeding new varieties but also lays foundation enriching

Язык: Английский

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Development and identification of two novel wheat-rye 6R derivative lines with adult-plant resistance to powdery mildew and high-yielding potential

The Crop Journal, Год журнала: 2023, Номер 12(1), С. 308 - 313

Опубликована: Окт. 5, 2023

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that seriously threatens wheat yield and quality. To control this disease, host resistance the most effective measure. Compared with genes from common wheat, alien can better withstand infection of highly variable pathogen. Development elite germplasm resources powdery mildew other key breeding traits an attractive strategy in breeding. In study, three wheat-rye lines YT4-1, YT4-2, YT4-3 were developed through hybridization between octoploid triticale out which YT4-1 YT4-2 conferred adult-plant (APR) to while line was susceptible during all its growth stages. Using genomic situ hybridization, multi-color fluorescence GISH, molecular marker analysis, shown be cytogenetically stable 6R addition T1RS·1BL translocation line, 6RL ditelosomic respectively. previously reported derivative carrying chromosome 6R, showed APR without undesirable pleiotropic effects on agronomic traits. Therefore, these novel are expected promising bridge

Язык: Английский

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The wheat NLR pair RXL/Pm5e confers resistance to powdery mildew

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Summary Powdery mildew poses a significant threat to global wheat production and most cloned deployed resistance genes for breeding encode nucleotide‐binding leucine‐rich repeat (NLR) immune receptors. Although two genetically linked NLRs function together as an NLR pair have been reported in other species, this phenomenon has relatively less studied wheat. Here, we demonstrate that tightly genes, RXL Pm5e , arranged head‐to‐head orientation, mediate powdery The of the / is validated by mutagenesis, gene silencing, gene‐editing assays. Interestingly, both atypical NLRs, with possessing truncated NB‐ARC (nucleotide binding adaptor shared APAF‐1, plant R proteins CED‐4) domain featuring coiled‐coil (CC) domain. Notably, lack integrated associated effector recognition found all previously pairs. Additionally, exhibit preference forming hetero‐complexes rather than homo‐complexes, highlighting their cooperative role disease resistance. We further show CC specifically suppresses hypersensitive response induced through competitive interaction, revealing regulatory mechanisms within pair. Our study sheds light on molecular mechanism underlying Pm5e‐ mediated provides new example

Язык: Английский

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Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Фев. 6, 2025

Common wheat is a leading global food crop that impacts security. Wheat powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), poses significant threat to grain yield and flour quality. The identification utilization of broad-spectrum resistance genes against PM are essential for effective disease control. spectrum test during the seedling stage adult were conducted evaluate landrace Honghuamai (HHM). Five segregating populations investigated assess inheritance in HHM. To map its resitance gene, bulked segregant analysis, molecular mapping comparative genomic analysis also used present study. HHM shows remarkable field nearly immune all 25 Bgt isolates tests, making it an excellent source resistance. was determined single dominant temporarily named PmHHM. It then fine-mapped interval with genetic distance 0.0031 cM physical 187.4 kb on chromosome 4AL Chinese Spring reference sequence v.2.1. Four identified target region, three which encode nucleotide-binding leucine-rich repeat (NLR) proteins. Comparative revealed presence/absence variations (PAVs) PmHHM locus among common varieties. These closely linked markers will not only benefit cloning gene underlying but facilitate efficient breeding programs.

Язык: Английский

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Progress and innovations of gene cloning in wheat and its close relatives

Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(5)

Опубликована: Апрель 29, 2025

Abstract Key message Wheat and its close relatives have large complex genomes, making gene cloning difficult. Nevertheless, developments in genomics over the past decade made it more feasible. The genomes of cereals, especially bread wheat, always been a challenge for mapping cloning. recent advances led to significant progress this field. Currently, high-quality reference sequences are available major wheat species their relatives. New high-throughput genotyping platforms next-generation sequencing technologies combined with genome complexity reduction techniques mutagenesis opened new avenues In review, we provide comprehensive overview genes cloned so far discuss strategies used these genes. We highlight advantages drawbacks individual approaches show how particular genomic contributed A wide range resources increase number successful projects decade, demonstrating that is now feasible perform rapid agronomically important genes, even as wheat.

Язык: Английский

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Crosstalk of Jasmonic acid and Salicylic acid with other Phytohormones Alleviates Abiotic and Biotic Stresses in Plants

Journal of soil science and plant nutrition, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Язык: Английский

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Introgression of an All-Stage and Broad-Spectrum Powdery Mildew Resistance Gene Pm3VS from Dasypyrum villosum Chromosome 3V into Wheat

Plant Disease, Год журнала: 2024, Номер 108(7), С. 2073 - 2080

Опубликована: Фев. 23, 2024

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a serious disease that threatens wheat production globally. It imperative to explore novel resistance genes control this developing and planting resistant varieties. Here, we identified wheat-Dasypyrum villosum 3V (3D) disomic substitution line, NAU3815 (2n = 42), with high level of powdery mildew at both the seedling adult-plant stages. Subsequently, was used generate recombination between chromosomes 3D. Through genomic in situ hybridization (GISH), fluorescence (FISH), 3VS- 3VL-specific markers analysis, four introgression lines were developed from selfing progenies 3D double monosomic line NAU3816, which derived F 1 hybrids NAU3815/NAU0686. There t3VS ditelosomic NAU3817, t3VL NAU3818, homozygous T3DL·3VS translocation NAU3819, T3DS·3VL NAU3820. tests these confirmed presence an all-stage broad-spectrum gene, Pm3VS, located on chromosome arm 3VS. When compared recurrent parent NAU0686 plants, NAU3819 showed no obvious negative effect yield-related traits. However, introduction translocated had strong reducing flag-leaf length. Consequently, provides new germplasm breeding for plant architecture.

Язык: Английский

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Mapping of powdery mildew resistance genes transferred to common wheat from wild emmer wheat revealed three functional Pm60 haplotypes

The Crop Journal, Год журнала: 2024, Номер 12(2), С. 540 - 548

Опубликована: Фев. 29, 2024

Powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), is one of the destructive wheat diseases worldwide. Wild emmer (Triticum turgidum ssp. dicoccoides, WEW), a tetraploid progenitor common wheat, valuable genetic resource for disease resistance breeding programs. We developed three hexaploid pre-breeding lines with PM genes derived from WEW accessions. These resistant were crossed susceptible Segregations in F2 populations 3 resistant: 1 susceptible, suggesting single dominant allele each parent. Mapping gene line indicated locus on long arm chromosome 7A, at approximate location previously cloned Pm60 T. urartu. Sanger sequencing revealed different haplotypes (Hap 3, Hap 5, and 6). Co-segregating diagnostic markers identification selection haplotype. The function haplotype was verified virus-induced silencing (VIGS). Common carrying these to most Bgt isolates differences response arrays suggested allelic variation response.

Язык: Английский

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An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 27, 2024

Язык: Английский

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Development of wheat-tetraploid Thinopyrum elongatum 4EL small fragment translocation lines with stripe rust resistance gene Yr4EL

Theoretical and Applied Genetics, Год журнала: 2024, Номер 137(10)

Опубликована: Окт. 1, 2024

Язык: Английский

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