Journal of Integrative Plant Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 28, 2025
ABSTRACT
Transcription
activator‐like
effectors
(TALEs)
mimic
eukaryotic
transcriptional
activators
and
translocate
into
host
plant
cells
via
the
bacterial
type
III
secretion
system
(T3SS)
during
pathogenic
interactions.
They
play
a
crucial
role
in
disease
development
by
regulating
genes.
Despite
this,
regulatory
mechanisms
which
TALEs
control
OsWRKY
transcription
factors
(TFs)
remain
poorly
understood.
In
this
study,
we
show
that
two
from
Xanthomonas
oryzae
pv.
(
Xoo
)
individually
modulate
TFs,
resulting
increased
susceptibility
reduced
defense.
Specifically,
Xoo1219
Xoo2145
activate
expression
of
OsWRKY104
OsWRKY55
,
respectively,
through
direct
increases
to
activating
OsSWEET11
OsSWEET14
while
suppresses
defense
against
directly
OsWRKY62
.
These
findings
suggest
hijack
host's
TFs
create
favorable
environment
for
survival.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 8, 2024
Abstract
Bacterial
Blight
of
Cotton
(BBC)
caused
by
Xanthomonas
citri
pv.
malvacearum
(
Xcm
)
is
an
important
and
destructive
disease
affecting
cotton
plants.
Transcription
activator-like
effectors
(TALEs)
released
the
pathogen
regulate
resistance
to
susceptibility.
In
this
study,
we
sequenced
whole
genome
Xss-V
2
-18
identified
eight
tal
genes;
seven
on
plasmids
one
chromosome.
Deletion
complementation
experiments
genes
demonstrated
that
Tal1b
required
for
full
virulence
cotton.
Transcriptome
profiling
coupled
with
TALE-binding
element
prediction
revealed
targets
GhSWEET15A04/D04
GhSWEET15D02
simultaneously.
Expression
analysis
confirmed
independent
inducibility
Tal1b,
whereas
additionally
targeted
Tal1.
Moreover,
GUS
(β
-glucuronidase
Xa10
-mediated
HR
(hypersensitive
response)
assays
indicated
EBEs
are
direct
specific
activation
candidate
Tal1
Ta1b.
These
findings
may
advance
our
understanding
dynamics
between
TALEs
EBEs,
decipher
a
simple
effective
DNA-binding
mechanism
could
lead
development
more
efficient
methods
gene
editing
transgenic
research.
