Metatranscriptomic insights into the dengue patient blood microbiome: Enhanced microbial diversity and metabolic activity in severe patients DOI Creative Commons

Aanchal Yadav,

Priti Devi,

Pallawi Kumari

и другие.

PLoS neglected tropical diseases, Год журнала: 2024, Номер 18(10), С. e0012589 - e0012589

Опубликована: Окт. 17, 2024

Background Dengue is the most re-emergent infection, with approximately 100 million new cases reported annually, yet no effective treatment or vaccine exists. Here, we aim to define microbial community structure and their functional profiles in dengue positive patients varying disease severity. Methodology/Principal findings Hospital admitted 112 dengue-positive blood samples were analyzed by dual RNA-sequencing simultaneously identify transcriptionally active microbes (TAMs), expressed genes associated pathways. Results highlight that severe exhibited increased diversity presence of opportunistic species (unique core) which includes Bacillus cereus , Burkholderia pseudomallei Streptococcus suis Serratia marcescens . The profile analysis revealed enriched metabolic pathways such as protein degradation, nucleotide biosynthesis, ion transport, cell shape integrity, ATP formation cases, indicating high energy demands adaptability these microbes. Conclusion Our metatranscriptomic approach provides a species-level characterization microbiome composition reveals heightened TAMs dengue, underscoring need for further research into role microbiota progression. Comparing signatures across severity classes early offers unique potential convenient diagnosis infection.

Язык: Английский

Metatranscriptomic insights into the dengue patient blood microbiome: Enhanced microbial diversity and metabolic activity in severe patients DOI Creative Commons

Aanchal Yadav,

Priti Devi,

Pallawi Kumari

и другие.

PLoS neglected tropical diseases, Год журнала: 2024, Номер 18(10), С. e0012589 - e0012589

Опубликована: Окт. 17, 2024

Background Dengue is the most re-emergent infection, with approximately 100 million new cases reported annually, yet no effective treatment or vaccine exists. Here, we aim to define microbial community structure and their functional profiles in dengue positive patients varying disease severity. Methodology/Principal findings Hospital admitted 112 dengue-positive blood samples were analyzed by dual RNA-sequencing simultaneously identify transcriptionally active microbes (TAMs), expressed genes associated pathways. Results highlight that severe exhibited increased diversity presence of opportunistic species (unique core) which includes Bacillus cereus , Burkholderia pseudomallei Streptococcus suis Serratia marcescens . The profile analysis revealed enriched metabolic pathways such as protein degradation, nucleotide biosynthesis, ion transport, cell shape integrity, ATP formation cases, indicating high energy demands adaptability these microbes. Conclusion Our metatranscriptomic approach provides a species-level characterization microbiome composition reveals heightened TAMs dengue, underscoring need for further research into role microbiota progression. Comparing signatures across severity classes early offers unique potential convenient diagnosis infection.

Язык: Английский

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