Phylogenetic analysis of the caspase family in bivalves: implications for programmed cell death, immune response and development
BMC Genomics,
Год журнала:
2021,
Номер
22(1)
Опубликована: Янв. 25, 2021
Abstract
Background
Apoptosis
is
an
important
process
for
organism’s
innate
immune
system
to
respond
pathogens,
while
also
allowing
cell
differentiation
and
other
essential
life
functions.
Caspases
are
one
of
the
key
protease
enzymes
involved
in
apoptotic
process,
however
there
currently
a
very
limited
understanding
bivalve
caspase
diversity
function.
Results
In
this
work,
we
investigated
presence
homologues
using
combination
bioinformatics
phylogenetic
analyses.
We
blasted
Crassostrea
gigas
genome
identified
35
potential
addition
already
cloned
23
caspases.
As
such,
present
information
about
relationship
all
caspases
relation
their
homology
well-established
vertebrate
invertebrate
Our
results
reveal
unexpected
novelty
complexity
family.
Notably,
were
unable
identify
direct
initiator
caspase-9,
key-caspase
pathway,
inflammatory
(caspase-1,
−
4
or
5)
executioner
caspases-3,
6,
7.
explored
fact
that
bivalves
appear
possess
several
unique
groups
2
8.
Large
expansions
caspase-3
like
(caspase-3A-C),
caspase-3/7
group
caspase-3/7-like
identified,
suggesting
unusual
roles
with
implications
our
response
common
diseases.
Furthermore,
assessed
gene
expression
two
(Cg2A,
Cg8B)
four
(Cg3A,
Cg3B,
Cg3C,
Cg3/7)
C.
late-larval
development
during
metamorphosis,
indicating
varies
across
different
developmental
stages.
Conclusion
analysis
provides
first
overview
species
new
insights
into
diversity,
knowledge
can
be
used
further
investigations
pathogens
regulation
processes.
Язык: Английский
Neuroendocrine regulation of the metamorphic transition of giant clams
Aquaculture,
Год журнала:
2025,
Номер
unknown, С. 742563 - 742563
Опубликована: Апрель 1, 2025
Язык: Английский
The unique biology of catch muscles: insights into structure, function, and robotics innovations
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
13
Опубликована: Апрель 16, 2025
The
Review
covers
the
current
state
of
functions,
neurotransmitter
innervation,
structure,
and
development
contractile
apparatus
unique
group
catch
muscles
inherent
only
to
bivalves.
In
contrast
conventional
muscles,
during
contraction
relaxation,
possess
a
ability
enter
holding
state,
referred
as
state.
latter
consists
in
energy-efficient
maintenance
long-lasting
tension
developed
by
muscle
without
consuming
ATP-derived
energy
regulated
serotonin
acetylcholine.
Despite
molecular
mechanism
phenomenon
still
remains
unclear,
combination
experimental
data
resulting
assumptions
allow
one
design
new
chemically-driven
artificial
muscles.
analysis
structure
function
this
work
opens
way
conceptually
strategy
for
biomimetic
robotics,
including
underwater
robotics.
Язык: Английский
Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas
BMC Developmental Biology,
Год журнала:
2020,
Номер
20(1)
Опубликована: Ноя. 23, 2020
Abstract
Background
Nitric
oxide
(NO)
is
presumed
to
be
a
regulator
of
metamorphosis
in
many
invertebrate
species,
and
although
NO
pathways
have
been
comparatively
well-investigated
gastropods,
annelids
crustaceans,
there
has
very
limited
research
on
the
effects
bivalve
shellfish.
Results
In
this
paper,
we
investigate
pathway
inhibitors
donors
induction
larvae
Pacific
oyster,
Crassostrea
gigas.
The
nitric
oxides
synthase
(NOS)
s-methylisothiourea
hemisulfate
salt
(SMIS),
aminoguanidine
(AGH)
7-nitroindazole
(7-NI)
induced
at
75,
76
83%
respectively,
operating
concentration-dependent
manner.
Additional
up
54%
resulted
from
exposures
1H-[1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one
(ODQ),
an
inhibitor
soluble
guanylyl
cyclase,
with
which
interacts
catalyse
synthesis
cyclic
guanosine
monophosphate
(cGMP).
Conversely,
high
concentrations
donor
sodium
nitroprusside
dihydrate
combination
inducers
epinephrine,
MK-801
or
SMIS,
significantly
decreased
metamorphosis,
potential
harmful
effect
excessive
unrelated
cannot
excluded.
Expression
CgNOS
also
after
regardless
used,
but
intensified
again
post-metamorphosis
spat.
Fluorescent
detection
competent
DAF-FM
diacetate
localisation
oyster
expression
by
in-situ
hybridisation
showed
that
occurs
primarily
two
key
larval
structures,
velum
foot.
cGMP
was
detected
foot
using
immunofluorescent
assays,
potentially
involved
foot’s
smooth
muscle
relaxation.
Conclusion
Together,
these
results
suggest
acts
as
negative
larvae,
reduction
induces
inhibiting
swimming
crawling
behaviour,
conjunction
cascade
additional
neuroendocrine
downstream
responses.
Язык: Английский
Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (Crassostrea gigas)
Marine Life Science & Technology,
Год журнала:
2023,
Номер
5(4), С. 467 - 477
Опубликована: Ноя. 25, 2023
Язык: Английский
A whole nervous system atlas of glutamate receptors reveals distinct receptor roles in sensorimotor circuits
Cezar Borba,
Matthew J. Kourakis,
Yishen Miao
и другие.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 19, 2023
Abstract
A
goal
of
connectomics
is
to
reveal
the
links
between
neural
circuits
and
behavior.
Larvae
primitive
chordate
Ciona
are
well-suited
make
contributions
in
this
area.
The
small
size
larval
nervous
system
(∼180
neurons)
has
facilitated
full
characterization
a
connectome.
In
addition,
larvae
display
range
behaviors
that
readily
quantified
both
normal
manipulated
larvae.
Moreover,
number
neurons
allows
for
neuron-by-neuron
attributes
such
as
neurotransmitter
use.
We
present
here
system-wide
atlas
glutamate
receptor
expression.
Included
ionotropic
receptors
(AMPA,
NMDA,
Kainate),
metabotropic
receptors.
expression
these
presented
context
known
driving
phototaxis,
mechanosensation,
looming
shadow
response.
AMPA
NMDA
receptors,
particular,
helps
resolve
apparently
paradoxical
coproduction
GABA
by
some
photoreceptors.
find
targets
photoreceptors,
midbrain
relay
neurons,
primarily
express
absence
This
agreement
with
previous
results
indicating
primary
from
photoreceptors
evoking
behavioral
response
(swimming)
through
disinhibition
mechanism.
hypothesize
have
modulatory
effect
neurons.
Other
findings
reported
more
unexpected.
For
example,
glutamatergic
epidermal
sensory
(ESNs)
do
not
any
yet
ESNs
themselves
Thus,
we
speculate
their
production
may
be
communication
neighboring
ESNs,
rather
than
interneuron
targets.
Язык: Английский
The Mediterranean mussel,Mytilus galloprovincialis, a novel model for developmental studies of mollusks
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 28, 2023
Abstract
A
model
organism
in
developmental
biology
is
defined
by
its
experimental
amenability
as
well
resources
created
for
the
system
scientific
community.
For
most
powerful
models,
combination
of
both
has
already
yielded
a
thorough
understanding
development.
However,
number
systems
still
very
limited,
and
their
phylogenetic
distribution
heavily
biased.
Members
one
largest
animal
phyla,
mollusks,
example,
have
long
been
neglected
organisms.
To
remedy
this
shortcoming,
we
produced
detailed
transcriptome
Mediterranean
mussel
Mytilus
galloprovincialis
,
bivalve
mollusk,
expanded
list
protocols
available
species.
Our
high-quality
allowed
us
to
identify
transcriptomic
signatures
transitions
perform
first
comparison
with
Pacific
oyster
Crassostrea
gigas
that
can
be
used
future
multi-species
analyses.
allow
co-labelling
studies,
optimized
immunohistochemistry
hybridization
chain
reaction
combined
techniques
create
high-resolution
co-expression
maps
genes.
The
describe
here
thus
represent
an
enormous
boost
establishment
laboratory
biology.
Summary
statement
Resources
are
described
which,
together,
establish
novel
studying
mollusk
development
evolution.
Язык: Английский