Rejection of inappropriate synaptic partners mediated by transcellular FLRT2-UNC5 signaling DOI Creative Commons

Cameron L. Prigge,

Arsha Sharma,

Mayur Dembla

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Авг. 30, 2022

ABSTRACT During nervous system development, neurons choose synaptic partners with remarkable specificity; however, the cell-cell recognition mechanisms governing rejection of inappropriate remain enigmatic. Here we show that mouse retinal avoid using FLRT2-UNC5 receptor-ligand system. Within inner plexiform layer (IPL), FLRT2 is expressed by direction-selective (DS) circuit neurons, whereas UNC5C/D are non-DS projecting to adjacent IPL sublayers. In vivo gain- and loss-of-function experiments demonstrate binding eliminates growing DS dendrites have strayed from Abrogation allows mistargeted arbors persist, elaborate, acquire synapses partners. Conversely, UNC5C misexpression within sublayers inhibits dendrite growth drives into Mechanistically, UNC5s promote elimination interfering FLRT2-mediated adhesion. Based on their broad expression, FLRT-UNC5 poised exert widespread effects upon partner choices across

Язык: Английский

Rejection of inappropriate synaptic partners mediated by transcellular FLRT2-UNC5 signaling DOI Creative Commons

Cameron L. Prigge,

Arsha Sharma,

Mayur Dembla

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Авг. 30, 2022

ABSTRACT During nervous system development, neurons choose synaptic partners with remarkable specificity; however, the cell-cell recognition mechanisms governing rejection of inappropriate remain enigmatic. Here we show that mouse retinal avoid using FLRT2-UNC5 receptor-ligand system. Within inner plexiform layer (IPL), FLRT2 is expressed by direction-selective (DS) circuit neurons, whereas UNC5C/D are non-DS projecting to adjacent IPL sublayers. In vivo gain- and loss-of-function experiments demonstrate binding eliminates growing DS dendrites have strayed from Abrogation allows mistargeted arbors persist, elaborate, acquire synapses partners. Conversely, UNC5C misexpression within sublayers inhibits dendrite growth drives into Mechanistically, UNC5s promote elimination interfering FLRT2-mediated adhesion. Based on their broad expression, FLRT-UNC5 poised exert widespread effects upon partner choices across

Язык: Английский

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